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EasySep™小鼠CD4正选试剂盒II

免疫磁珠正选小鼠CD4+细胞的分选试剂盒
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产品号 #（选择产品）

产品号 #18952_C

免疫磁珠正选小鼠CD4+细胞的分选试剂盒

产品优势

  • 快捷，易于操作，且无需分离柱
  • 纯度高达98%
  • 分选得到的细胞无荧光标记

产品组分包括

  • EasySep™小鼠CD4正选试剂盒II（产品号 #18952）
    • EasySep™小鼠CD4正选试剂盒组分A，0.5mL
    • EasySep™小鼠CD4正选试剂盒组分B，0.5mL
    • 小鼠FcR多克隆阻断剂（产品号#300-0902），1.2mL
    • EasySep™ Dextran RapidSpheres™ 50100 磁珠，1mL
  • RoboSep™小鼠CD4正选试剂盒II（产品号 #18952RF）
    • EasySep™小鼠CD4正选试剂盒组分A，0.5mL
    • EasySep™小鼠CD4正选试剂盒组分B，0.5mL
    • 小鼠FcR多克隆阻断剂（产品号#300-0902），1.2mL
    • EasySep™ Dextran RapidSpheres™ 50100 磁珠，1mL
    • RoboSep™空管
    • RoboSep™ 缓冲液（产品号 #20104）
    • RoboSep™过滤吸头（产品号#20125）x 2
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要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。
  1. EasyEights™ EasySep™磁极
    EasyEights™ EasySep™磁极
  2. EasySep™缓冲液
    EasySep™缓冲液
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

EasySep™小鼠CD4正选试剂盒II，通过免疫磁珠正选技术，可轻松从小鼠脾细胞或其他组织样本的单细胞悬液中分离高纯度的小鼠CD4+细胞。EasySep™技术结合单克隆抗体的特异性和无需分离柱的简便磁珠分选系统，已在发表的研究中广泛应用超过20年。

在本款EasySep™正选流程中，目的细胞通过与识别CD4的抗体复合物及磁珠进行标记。使用EasySep™磁珠分选系统分离标记细胞，只需倾倒或移液吸取非目的细胞。目的细胞保留在试管中。分选后的目的CD4+细胞即可用于流式细胞术、细胞培养及基于细胞的实验等下游应用。

了解更多关于EasySep™免疫磁珠技术的工作原理，或如何通过RoboSep™实现免疫磁珠细胞分选全自动化。探索为您的实验流程优化的更多产品，包括培养基、添加物、抗体等。

 

磁极兼容性
• EasySep™磁极（产品号 #18000）
• “The Big Easy” EasySep™磁极（产品号 #18001）
• EasyEights™ EasySep™磁极（产品号 #18103）
• RoboSep™-S（产品号 #21000）
 
分类
细胞分选试剂盒
 
细胞类型
T 细胞，CD4+ T细胞
 
种属
小鼠
 
样本来源
其他组织，脾脏
 
分选方法
正选
 
应用
细胞分选
 
品牌
EasySep，RoboSep
 
研究领域
免疫
 

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实验数据

Typical EasySep™ CD4 Positive Cell Isolation Profile

Figure 1. Typical EasySep™ CD4 Positive Cell Isolation Profile

Starting with mouse splenocytes, the CD4+ cell content of the isolated fraction is typically 94.8 ± 3.5% (mean ± SD) for the purple EasySep™ Magnet. In the above example, the purities of the start and final isolated fractions are 20.7% and 96.2%, respectively.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
产品说明书
Product Name
RoboSep™ 小鼠CD4正选试剂盒II
Catalog #
18952RF
Lot #
1000170430 or higher
Language
中文
Document Type
产品说明书
Product Name
EasySep™小鼠CD4正选试剂盒II
Catalog #
18952
Lot #
1000170430 or higher
Language
中文
Document Type
Product Information Sheet 1
Product Name
RoboSep™ Mouse CD4 Positive Selection Kit II
Catalog #
18952RF
Lot #
1000170429 or lower
Language
English
Document Type
Product Information Sheet 2
Product Name
RoboSep™ Mouse CD4 Positive Selection Kit II
Catalog #
18952RF
Lot #
1000170430 or higher
Language
English
Document Type
Product Information Sheet 1
Product Name
EasySep™ Mouse CD4 Positive Selection Kit II
Catalog #
18952
Lot #
1000170429 or lower
Language
English
Document Type
Product Information Sheet 2
Product Name
EasySep™ Mouse CD4 Positive Selection Kit II
Catalog #
18952
Lot #
1000170430 or higher
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Mouse CD4 Positive Selection Kit II
Catalog #
18952RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Mouse CD4 Positive Selection Kit II
Catalog #
18952RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Mouse CD4 Positive Selection Kit II
Catalog #
18952RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
RoboSep™ Mouse CD4 Positive Selection Kit II
Catalog #
18952RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
RoboSep™ Mouse CD4 Positive Selection Kit II
Catalog #
18952RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 6
Product Name
RoboSep™ Mouse CD4 Positive Selection Kit II
Catalog #
18952RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Mouse CD4 Positive Selection Kit II
Catalog #
18952
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Mouse CD4 Positive Selection Kit II
Catalog #
18952
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Mouse CD4 Positive Selection Kit II
Catalog #
18952
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
EasySep™ Mouse CD4 Positive Selection Kit II
Catalog #
18952
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

相关材料与文献

技术资料 (10)

EasySep™细胞分选技术 - 更高效地分离细胞
产品手册
EasySep™细胞分选技术 - 更高效地分离细胞
小鼠细胞分选
产品手册
小鼠细胞分选
细胞分选相关工具
产品手册
细胞分选相关工具
Mouse Immune Cell Isolation Course
点播课程
Mouse Immune Cell Isolation Course
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
视频
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ Magnet
视频
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ Magnet
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
视频
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Frequencies and Percentages of Mouse Immune Cell Types
挂图
Frequencies and Percentages of Mouse Immune Cell Types
Human Immune Cytokines
挂图
Human Immune Cytokines
Streamline Your Tissue Processing Using the Automated STEMprep™ Tissue Dissociator
线上讲座
Streamline Your Tissue Processing Using the Automated STEMprep™ Tissue Dissociator

常见问题

Can EasySep™ be used for either positive or negative selection?

Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).

How does the separation work?

Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Can EasySep™ be used to isolate rare cells?

Yes. We recommend a cell concentration of 2x108 cells/mL and a minimum working volume of 100 µL. Samples containing 2x107 cells or fewer should be suspended in 100 µL of buffer.

Are the EasySep™ magnetic particles FACS-compatible?

Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.

Can the EasySep™ magnetic particles be removed after enrichment?

No, but due to the small size of these particles, they will not interfere with downstream applications.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

For positive selection, can I perform more than 3 separations to increase purity?

Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

文献 (10)

Opposing Development of Cytotoxic and Follicular Helper CD4 T Cells Controlled by the TCF-1-Bcl6 Nexus. Donnarumma T et al. Cell reports 2016 NOV

Abstract

CD4(+) T cells develop distinct and often contrasting helper,regulatory,or cytotoxic activities. Typically a property of CD8(+) T cells,granzyme-mediated cytotoxic T cell (CTL) potential is also exerted by CD4(+) T cells. However,the conditions that induce CD4(+) CTLs are not entirely understood. Using single-cell transcriptional profiling,we uncover a unique signature of Granzyme B (GzmB)(+) CD4(+) CTLs,which distinguishes them from other CD4(+) T helper (Th) cells,including Th1 cells,and strongly contrasts with the follicular helper T (Tfh) cell signature. The balance between CD4(+) CTL and Tfh differentiation heavily depends on the class of infecting virus and is jointly regulated by the Tfh-related transcription factors Bcl6 and Tcf7 (encoding TCF-1) and by the expression of the inhibitory receptors PD-1 and LAG3. This unique profile of CD4(+) CTLs offers targets for their study,and its antagonism by the Tfh program separates CD4(+) T cells with either helper or killer functions.
View publication
Astragaloside IV regulates differentiation and induces apoptosis of activated CD4+ T cells in the pathogenesis of experimental autoimmune encephalomyelitis. L. Yang et al. Toxicology and applied pharmacology 2018 OCT

Abstract

CD4+ T cells,especially T-helper (Th) cells (Th1,Th2 and Th17) and regulatory T cells (Treg) play pivotal role in the pathogenesis of multiple sclerosis (MS),a demyelinating autoimmune disease occurring in central nervous system (CNS). Astragaloside IV (ASI,CAS: 84687-43-4) is one of the saponins isolated from Astragalus membranceus,a traditional Chinese medicine with immunomodulatory effect. So far,whether ASI has curative effect on experimental autoimmune encephalomyelitis (EAE),an animal model of MS,and how it affects the subsets of CD4+ T cells,as well as the underlying mechanism have not been clearly elucidated. In the present study,ASI was found to ameliorate the progression and hamper the recurrence of EAE effectively in the treatment regimens. It significantly reduced the demyelination and inflammatory infiltration of CNS in EAE mice by suppressing the percentage of Th1 and Th17 cells,which was closely associated with the inhibition of JAK/STAT and NF-$\kappa$B signaling pathways. ASI also increased the percentage of Treg cells in spleen and CNS,which was accompanied by elevated Foxp3. However,in vitro experiments disclosed that ASI could regulate the differentiation of Th17 and Treg cells but not Th1 cells. In addition,it induced the apoptosis of MOG-stimulated CD4+ T cells probably through modulating STAT3/Bcl-2/Bax signaling pathways. Together,our findings suggested that ASI can modulate the differentiation of autoreactive CD4+ T cells and is a potential prodrug or drug for the treatment of MS and other similar autoimmune diseases.
View publication
Macrophage Coordination of the Interferon Lambda Immune Response. S. A. Read et al. Frontiers in immunology 2019

Abstract

Lambda interferons (IFN-$\lambda$s) are a major component of the innate immune defense to viruses,bacteria,and fungi. In human liver,IFN-$\lambda$ not only drives antiviral responses,but also promotes inflammation and fibrosis in viral and non-viral diseases. Here we demonstrate that macrophages are primary responders to IFN-$\lambda$,uniquely positioned to bridge the gap between IFN-$\lambda$ producing cells and lymphocyte populations that are not intrinsically responsive to IFN-$\lambda$. While CD14+ monocytes do not express the IFN-$\lambda$ receptor,IFNLR1,sensitivity is quickly gained upon differentiation to macrophages in vitro. IFN-$\lambda$ stimulates macrophage cytotoxicity and phagocytosis as well as the secretion of pro-inflammatory cytokines and interferon stimulated genes that mediate immune cell chemotaxis and effector functions. In particular,IFN-$\lambda$ induced CCR5 and CXCR3 chemokines,stimulating T and NK cell migration,as well as subsequent NK cell cytotoxicity. Using immunofluorescence and cell sorting techniques,we confirmed that human liver macrophages expressing CD14 and CD68 are highly responsive to IFN-$\lambda$ ex vivo. Together,these data highlight a novel role for macrophages in shaping IFN-$\lambda$ dependent immune responses both directly through pro-inflammatory activity and indirectly by recruiting and activating IFN-$\lambda$ unresponsive lymphocytes.
View publication
View All Publications

更多信息

更多信息
物种 小鼠
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
样本来源 其它细胞系, 脾脏
Selection Method Positive
标记抗体
  1. 抗小鼠CD4抗体，clone RM4-4
    抗小鼠CD4抗体，clone RM4-4

    抗小鼠CD4的大鼠Monoclonal IgG2b抗体

  2. 抗小鼠CD3e抗体，clone 145-2C11
    抗小鼠CD3e抗体，clone 145-2C11

    亚美尼亚仓鼠Monoclonal IgG1抗体，抗小鼠CD3e

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