若您需要咨询产品或有任何技术问题,请通过官方电话 400 885 9050 或邮箱 info.cn@stemcell.com 与我们联系。

EasySep™人细胞外囊泡(CD81)正选试剂盒

对来源于血浆、血清、细胞培养上清及尿液的人 CD81⁺ 细胞外囊泡(EVs)进行免疫磁珠正选分离

产品号 #(选择产品)

产品号 #17892_C

利用免疫磁珠正选分选快速简便分离人细胞外囊泡

产品优势

快速、简单、无柱 避免使用需要超速离心的长耗时方法分离EV。 获取高纯度EVs,不污染生物液体组分。

产品组分包括

EasySep™人细胞外囊泡(CD81)正选试剂盒 (产品号# 17892) EasySep™人细胞外囊泡(CD)正选抗体混合物,1*1mL EasySep™ Releasable RapidSpheres™ 50201磁珠, 2*1mL 注意:分离的细胞外囊泡不从EasySep™Releasable RapidSpheres™50201上解离
专为您的实验方案打造的产品
要查看实验方案所需的所有配套产品,请参阅《实验方案与技术文档》

总览

使用 EasySep™ 人外泌体(CD81)正选试剂盒,通过免疫磁珠正选,可轻松地从血浆、血清、细胞培养上清液和尿液样本中分离高纯度的人 CD81⁺ 外泌体(EVs)。EasySep™ 技术结合单克隆抗体的特异性与无柱磁系统的简便性,已在发表的研究中广泛应用超过 20 年。

在此 EasySep™ 正选过程中,目标 EV 通过识别四跨膜蛋白标志物 CD81 的抗体复合物与磁珠进行标记。使用EasySep™磁极进行无柱式分选,通过简单地倾倒或移去不需要的生物体液成分,目标 EV 保留在管中。整个磁珠分选过程可在 30 分钟内完成,分离得到的 CD81⁺ EV 可直接用于下游应用,如 DNA/RNA 提取、蛋白质印迹或质谱分析。该方法可获得低污染蛋白(如载脂蛋白和白蛋白)的 细胞外囊泡(EV),从而确保获得更纯净的样品,用于后续的灵敏分析。

了解更多关于 EasySep™ 免疫磁性技术的工作原理。探索更多优化您实验流程的相关产品,包括培养基、补充物、抗体等。

 

磁极兼容性
• EasySep™磁极(产品号 #18000)
• “The Big Easy” EasySep™磁极(产品号 #18001)
 
分类
细胞分选试剂盒
 
细胞类型
其他物种
 
样本来源
其他物种
 
分选方法
正选
 
应用
细胞分选,细胞外囊泡分选
 
品牌
EasySep
 
研究领域
细胞外囊泡研究
 

Data Figures

Positive isolation of EVs with the CD81 tetraspanin marker from human plasma and MSC-conditioned medium.

Figure 1. Typical Western Blot Analyses of EVs Isolated from Human Plasma and Mesenchymal Stromal Cell (MSC)-Conditioned Medium

The Western blot analyses in the above examples show positive isolation of EVs with the CD81 tetraspanin marker from (A) human plasma and (B) MSC-conditioned medium. Western blots were run under non-reducing conditions.

Figure 2. Tetraspanin Protein Expression and Recovery of EVs Using EasySep™ Human Pan/CD9/CD63/CD81 Extracellular Vesicle Positive Selection Kits

EVs were isolated from plasma using either EasySep™ Human Extracellular Vesicle Positive Selection Kits or differential ultracentrifugation (2 x 70 min, 100,000 xg) and isolated fractions were analyzed by western blot for tetraspanin protein expression.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
17892
Lot #
All
Language
English
Catalog #
17892
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
17892
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
17892
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

更多信息

更多信息
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001)
样本来源 其它细胞系
Selection Method Positive
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
Copyright © 2025 by STEMCELL Technologies. All rights reserved.

在线联系