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EasySep™Magnet支架

EasySep™Magnet的可连接支架
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产品号 #18130_C

EasySep™Magnet的可连接支架

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产品说明书及文档
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总览

使用 EasySep™ EasyStand™ 与 EasySep™ 磁极（产品号 #18000）及选定的 EasySep™ 试剂，可轻松高效地进行磁性细胞分离。每个 EasySep™ EasyStand™ 可放置一个 EasySep™ 磁极，并可通过简易系统将多达 6 个 EasySep™ EasyStand™ 单元连接在一起，从而同时处理一个或多个样本。每轮磁珠分离后，所有样本的上清液可同时倒入接收管中。

不确定应使用哪种磁极？请访问 EasySep™ 细胞分离磁极页面比较不同选项并选择最适合您研究的磁极。

了解更多关于 EasySep™ 免疫磁性细胞分选技术的工作原理。

 

种属
人，小鼠，非人灵长类，其他物种，大鼠
 
应用
细胞分选
 
品牌
EasySep
 

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产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
EasySep™ EasyStand™
Catalog #
18130
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

相关材料与文献

技术资料 (3)

Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
1:37
Video
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep...
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology

常见问题

Can EasySep™ be used for either positive or negative selection?

Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).

How does the separation work?

Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Can EasySep™ be used to isolate rare cells?

Yes. We recommend a cell concentration of 2x108 cells/mL and a minimum working volume of 100 µL. Samples containing 2x107 cells or fewer should be suspended in 100 µL of buffer.

Are the EasySep™ magnetic particles FACS-compatible?

Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.

Can the EasySep™ magnetic particles be removed after enrichment?

No, but due to the small size of these particles, they will not interfere with downstream applications.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

For positive selection, can I perform more than 3 separations to increase purity?

Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

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更多信息
物种 人类, 其它物种, 大鼠, 小鼠, 非人灵长类

质量保证:

本产品仅供科研使用，除非另有说明，不得用于人体或动物的诊断或治疗用途。如需了解 STEMCELL 的质量体系，请访问 www.stemcell.cn/compliance

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