EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #07913_C
含 5 U/mL Dispase 的 Hanks’ 平衡盐溶液（HBSS）
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总览
Dispase 可用于多种组织的温和解离。将剪碎的组织与预温的 Dispase一起孵育并轻柔搅拌，将以最小的细胞损伤释放细胞。预温的 Dispase可用于从组织培养皿中分离细胞。该蛋白水解解离试剂已针对人类和小鼠乳腺组织的解离优化，可高效制备单细胞悬液。
本产品含有 5 U/mL 的 Dispase II（来源于多粘类芽孢杆菌的中性蛋白酶），溶于 Hanks’ 平衡盐溶液（HBSS）中。与胰蛋白酶不同，Dispase 不会被血清抑制，但其活性会受到 EDTA 和 EGTA 的抑制。使用后应通过离心去除 Dispase，并用缓冲液或培养基清洗细胞。
分类
酶法相关（或酶解类产品）
别名
中性蛋白酶；蛋白酶
细胞类型
肠道细胞，乳腺细胞，其他物种，多能干细胞，前列腺细胞
种属
人，小鼠，非人灵长类，其他物种，大鼠
应用
细胞培养
研究领域
上皮细胞研究，干细胞生物学
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (1)
文献 (13)
Derivation of 30 human embryonic stem cell lines-improving the quality
In vitro cellular & developmental biology. Animal 2010 APR
Abstract
We have derived 30 human embryonic stem cell lines from supernumerary blastocysts in our laboratory. During the derivation process,we have studied new and safe method to establish good quality lines. All our human embryonic stem cell lines have been derived using human foreskin fibroblasts as feeder cells. The 26 more recent lines were derived in a medium containing serum replacement instead of fetal calf serum. Mechanical isolation of the inner cell mass using flexible metal needles was used in deriving the 10 latest lines. The lines are karyotypically normal,but culture adaptation in two lines has been observed. Our human embryonic stem cell lines are banked,and they are available for researchers.
Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA
Cell stem cell 2010 NOV
Abstract
Clinical application of induced pluripotent stem cells (iPSCs) is limited by the low efficiency of iPSC derivation and the fact that most protocols modify the genome to effect cellular reprogramming. Moreover,safe and effective means of directing the fate of patient-specific iPSCs toward clinically useful cell types are lacking. Here we describe a simple,nonintegrating strategy for reprogramming cell fate based on administration of synthetic mRNA modified to overcome innate antiviral responses. We show that this approach can reprogram multiple human cell types to pluripotency with efficiencies that greatly surpass established protocols. We further show that the same technology can be used to efficiently direct the differentiation of RNA-induced pluripotent stem cells (RiPSCs) into terminally differentiated myogenic cells. This technology represents a safe,efficient strategy for somatic cell reprogramming and directing cell fate that has broad applicability for basic research,disease modeling,and regenerative medicine. ?? 2010 Elsevier Inc.
Developmental and Functional Nature of Human iPSC Derived Motoneurons
Stem Cell Reviews and Reports 2013 AUG
Abstract
更多信息
| 物种 | 人, 其它物种, 大鼠, 小鼠, 非人灵长类 |
|---|---|
| Alternative Names | Neutral protease; Proteinase |
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