EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #72092_C
糖皮质激素通路激活剂；激活糖皮质激素受体
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总览
地塞米松是一种合成的糖皮质激素，类似于天然的糖皮质激素氢化可的松。与天然氢化可的松配体相比，地塞米松对糖皮质激素受体的亲和力更高（Kd = 5 nM vs 17 nM）。
重编程
·促进小鼠胰腺细胞向肝细胞的转分化（Shen et al.）。
分化
·促进人间充质细胞向成骨、脂肪和软骨方向分化（Jaiswal et al., Mackay et al., Pittenger et al.）。
·促进小鼠间充质细胞向成骨、脂肪和软骨方向分化（Tropel et al.）。
·促进小鼠和人胚胎干细胞（ES）向成熟肝细胞分化（Cai et al., Kubo et al.）。
·促进小鼠胎肝细胞成熟（Kamiya et al.）。
别名
MK 125，NSC 34521
细胞类型
间充质干/祖细胞，胰腺细胞，多能干细胞
种属
人，小鼠，非人灵长类，其他物种，大鼠
应用
分化，重编程
研究领域
上皮细胞研究，干细胞生物学
CAS 编号
50-02-2
化学式
C₂₂H₂₉FO₅
分子量
392.5 g/mol
纯度
≥98%
通路
糖皮质激素
靶点
糖皮质激素受体
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (4)
文献 (9)
Multilineage potential of adult human mesenchymal stem cells.
Science (New York,N.Y.) 1999 APR
Abstract
Human mesenchymal stem cells are thought to be multipotent cells,which are present in adult marrow,that can replicate as undifferentiated cells and that have the potential to differentiate to lineages of mesenchymal tissues,including bone,cartilage,fat,tendon,muscle,and marrow stroma. Cells that have the characteristics of human mesenchymal stem cells were isolated from marrow aspirates of volunteer donors. These cells displayed a stable phenotype and remained as a monolayer in vitro. These adult stem cells could be induced to differentiate exclusively into the adipocytic,chondrocytic,or osteocytic lineages. Individual stem cells were identified that,when expanded to colonies,retained their multilineage potential.
Fetal liver development requires a paracrine action of oncostatin M through the gp130 signal transducer.
The EMBO journal 1999 APR
Abstract
Fetal liver,the major site of hematopoiesis during embryonic development,acquires additional various metabolic functions near birth. Although liver development has been characterized biologically as consisting of several distinct steps,the molecular events accompanying this process are just beginning to be characterized. In this study,we have established a novel culture system of fetal murine hepatocytes and investigated factors required for development of hepatocytes. We found that oncostatin M (OSM),an interleukin-6 family cytokine,in combination with glucocorticoid,induced maturation of hepatocytes as evidenced by morphological changes that closely resemble more differentiated hepatocytes,expression of hepatic differentiation markers and intracellular glycogen accumulation. Consistent with these in vitro observations,livers from mice deficient for gp130,an OSM receptor subunit,display defects in maturation of hepatocytes. Interestingly,OSM is expressed in CD45(+) hematopoietic cells in the developing liver,whereas the OSM receptor is expressed predominantly in hepatocytes. These results suggest a paracrine mechanism of hepatogenesis; blood cells,transiently expanding in the fetal liver,produce OSM to promote development of hepatocytes in vivo.
Molecular basis of transdifferentiation of pancreas to liver.
Nature cell biology 2000 DEC
Abstract
The appearance of hepatic foci in the pancreas has been described in animal experiments and in human pathology. Here we show that pancreatic cells can be converted into hepatocytes by treatment with a synthetic glucocorticoid,dexamethasone. This occurs both in a pancreatic cell line,AR42J-B13,and in organ cultures of pancreatic buds from mouse embryos. We have established several features of the mechanism behind this transdifferentiation. We show that a proportion of the hepatocytes arises directly from differentiated exocrine-like cells,with no intervening cell division. This conversion is associated with induction of the transcription factor C/EBPbeta and the activation of differentiated hepatic products. Transfection of C/EBPbeta into the cells can provoke transdifferentiation; conversely,a dominant-negative form of C/EBPbeta can inhibit the process. These results indicate that C/EBPbeta is a key component that distinguishes the liver and pancreatic programmes of differentiation.
更多信息
| Molecular Weight | 392.5 g/mol |
|---|---|
| 物种 | 人, 其它物种, 大鼠, 小鼠, 非人灵长类 |
| Alternative Names | MK 125, NSC 34521 |
| Cas Number | 50-02-2 |
| Chemical Formula | C₂₂H₂₉FO₅ |
| 纯度 | ≥ 98% |
| Target | Glucocorticoid Receptor |
| Pathway | Glucocorticoid |
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质量保证:
产品仅供研究使用，不用于针对人或动物的诊断或治疗。
产品仅供研究使用，不用于针对人或动物的诊断或治疗。
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