STEMdiff™定型内胚层检测试剂盒

用于将人胚胎干细胞（hESCs）和人诱导多能干细胞（iPSCs）分化为确定性内胚层的定义明确、无动物源成分的培养基

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产品号 #05110_C

成分明确的无动物成分培养基，用于将人胚胎干细胞（ES）和诱导多能干细胞（iPS）分化为定型内胚层。

产品优势

成分明确、无血清、无动物成分的完整且即用型培养基，用于人胚胎干细胞和iPS细胞分化为定型内胚层
实现多种胚胎干细胞和iPS细胞系的高效和可复制分化
产生定型内胚层细胞，能够进一步分化为胰腺、肝脏、肠和肺细胞系

产品组分包括

STEMdiff™内胚层基础培养基，100 mL
STEMdiff™定型内胚层补充MR (100X), 0.35 mL
STEMdiff™定型内胚层补充CJ (100X), 1.1 mL

立即询价

总览

STEMdiff™ Definitive Endoderm Kit 是一套完整的无血清、无动物源成分的培养基与添加物剂盒，可高效支持人胚胎干细胞（hESC）和人诱导多能干细胞（hiPSC）向定向内胚层细胞分化。

使用 STEMdiff™ Definitive Endoderm Kit 分化的细胞高表达内胚层标志物，包括 CD184（CXCR4）、SOX17、FOXA2 和 c-KIT，同时不表达外胚层、中胚层及多能性标志物。通过该试剂盒获得的定向内胚层细胞具有多向分化潜能，可进一步分化为胰腺、肠道、肺及肝脏谱系细胞，因此是发育生物学研究、疾病建模及药物发现的强大工具。

本试剂盒针对在 mTeSR™1、mTeSR™ Plus 或 TeSR™-AOF 中维持培养的细胞的分化进行了优化。若需了解在 TeSR™-E8™ 中培养的细胞的分化，请参阅 STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™ Optimized)。

实验数据

Figure 1. Definitive endoderm differentiation is efficient across multiple human ES and iPS cell lines

Quantitative analysis of definitive endoderm formulation on multiple human ES and iPS cell lines as measured by co-expression of CXCR4 and SOX17. Prior to differentiation using STEMdiff™ Definitive Endoderm, cells were maintained in their pluripotent state by culturing mTeSR™1 on Matrigel. Data are expressed as the mean percent of cells expressing both markers. Error bars indicate SEM, n = 4-18 per cell line.

Figure 2. Quantitative Analysis of Definitive Endoderm hES and iPS-Derived Using STEMdiff™ Definitive Endoderm

Quantitative analysis of definitive endoderm in human ES and iPS cells previously maintained in TeSR™2 prior to differentiation on Matrigel using STEMdiff™ Definitive Endoderm. Data are expressed as the mean percent of cells expressing both markers. Error bars indicate SEM. n = 4-11 per cell line.

Figure 3. Efficient definitive endoderm differentiation in human ES and iPS cells

Representative Density plots showing CXCR4 and SOX17 expression in human ES cells (H1 and H9) and human iPS cells (WLS-4D1 and A13700) following 5 days of differentiation to definitive endoderm using STEMdiff™ Definitive Endoderm. Isotype controls were used to set quadrant gates.

Figure 4. STEMdiff™ Definitive Endoderm yields DE that retains potency for downstream lineage specification

Cultures differentiated using STEMdiff™ Definitive Endoderm maintain their ability to be directed towards pancreatic and hepatic lineages. A) Representative image of PDX-1 immunoreactivity in H9 cells following pancreatic specification. Scale bar 20 µm. B) Representative image of human serum albumin (HSA) immunoreactivity in H9 cells following hepatic specification. Scale bar, 100 µm.

Density plots and quantitative analysis showing CXCR4 and SOX17 expression in cells cultured in mTeSR™1 (daily feeds) or mTeSR™ Plus (restricted feeds), following 5 days of differentiation using the STEMdiff™ Definitive Endoderm Kit.

Figure 5. Generation of Definitive Endoderm from hPSCs Maintained in mTeSR™ Plus

(A) Representative density plots showing CXCR4 and SOX17 expression in cells cultured in mTeSR™1 (daily feeds) or mTeSR™ Plus (restricted feeds), following 5 days of differentiation using the STEMdiff™ Definitive Endoderm Kit. (B) Quantitative analysis of definitive endoderm formation in multiple hPSC lines (H9, STiPS-M001, WLS-1C) maintained with mTeSR™1 or mTeSR™ Plus as measured by co-expression of CXCR4 and SOX17. Data are expressed as the mean percentage of cells (± SEM) expressing both markers; n=3.

产品说明书及文档

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文献 (32)

Cell differentiation along multiple pathways accompanied by changes in histone acetylation status. Legartová et al. Biochemistry and cell biology = Biochimie et biologie cellulaire 2014 APR

Abstract

Post-translational modification of histones is fundamental to the regulation of basic nuclear processes and subsequent cellular events,including differentiation. In this study,we analyzed acetylated forms of histones H2A,H2B,and H4 during induced differentiation in mouse (mESCs) and human (hESCs) embryonic stem cells and during induced enterocytic differentiation of colon cancer cells in vitro. Endoderm-like differentiation of mESCs induced by retinoic acid and enterocytic differentiation induced by histone deacetylase inhibitor sodium butyrate were accompanied by increased mono-,di-,and tri-acetylation of histone H2B and a pronounced increase in di- and tri-acetylation of histone H4. In enterocytes,mono-acetylation of histone H2A also increased and tetra-acetylation of histone H4 appeared only after induction of this differentiation pathway. During differentiation of hESCs,we observed increased mono-acetylation and decreased tri-acetylation of H2B. Mono-,di-,and tri-acetylation of H4 were reduced,manifested by a significant increase in nonacetylated H4 histones. Levels of acetylated histones increased during induced differentiation in mESCs and during histone deacetylase (HDAC) inhibitor-induced enterocytic differentiation,whereas differentiation of human ESCs was associated with reduced acetylation of histones H2B and H4.

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Epigenome rejuvenation: HP1β mobility as a measure of pluripotent and senescent chromatin ground states. Manukyan M and Singh PB Scientific reports 2014 JAN

Abstract

We measured the dynamics of an essential epigenetic modifier,HP1β,in human cells at different stages of differentiation using Fluorescence Recovery After Photobleaching (FRAP). We found that HP1β mobility is similar in human embryonic stem cells (hES) and iPS cells where it is more mobile compared to fibroblasts; HP1β is less mobile in senescent fibroblasts than in young (dividing) fibroblasts. Introduction of reprogramming factors"�

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Human oocytes reprogram adult somatic nuclei of a type 1 diabetic to diploid pluripotent stem cells. Yamada M et al. Nature 2014 JUN

Abstract

The transfer of somatic cell nuclei into oocytes can give rise to pluripotent stem cells that are consistently equivalent to embryonic stem cells,holding promise for autologous cell replacement therapy. Although methods to induce pluripotent stem cells from somatic cells by transcription factors are widely used in basic research,numerous differences between induced pluripotent stem cells and embryonic stem cells have been reported,potentially affecting their clinical use. Because of the therapeutic potential of diploid embryonic stem-cell lines derived from adult cells of diseased human subjects,we have systematically investigated the parameters affecting efficiency of blastocyst development and stem-cell derivation. Here we show that improvements to the oocyte activation protocol,including the use of both kinase and translation inhibitors,and cell culture in the presence of histone deacetylase inhibitors,promote development to the blastocyst stage. Developmental efficiency varied between oocyte donors,and was inversely related to the number of days of hormonal stimulation required for oocyte maturation,whereas the daily dose of gonadotropin or the total number of metaphase II oocytes retrieved did not affect developmental outcome. Because the use of concentrated Sendai virus for cell fusion induced an increase in intracellular calcium concentration,causing premature oocyte activation,we used diluted Sendai virus in calcium-free medium. Using this modified nuclear transfer protocol,we derived diploid pluripotent stem-cell lines from somatic cells of a newborn and,for the first time,an adult,a female with type 1 diabetes.

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物种	人
配方	不含动物成分, 无血清

STEMdiff™定型内胚层检测试剂盒

产品号 #05110

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产品号 #05110_C

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STEMdiff™定型内胚层检测试剂盒

产品号 #05110

产品号 #（选择产品）

产品号 #05110_C

产品优势

产品组分包括

总览

实验数据

产品说明书及文档

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文献 (32)

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