EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #07930_C
无动物成分，含有10% DMSO的冻存液
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总览
使用BioLife Solutions的即用型CryoStor® CS10冻存液，可最大限度提高在极低温（-70°C至-196°C）冻存后细胞复苏率和存活率。该冻存液不含血清和动物成分，并按照 cGMP 标准生产，可提供安全有效的保护环境，推荐用于多种敏感细胞和样本类型的冷冻保存，包括骨髓瘤细胞系、人多能干细胞、血液来源细胞等。CryoStor® CS10提供多种便捷规格，采用USP级成分制备以减少差异，含10%DMSO。
包含
• 10% 二甲基亚砜 (DMSO)
• 其他成分
细胞类型
B 细胞，CHO细胞，造血干/祖细胞，杂交瘤细胞，肠道细胞，巨噬细胞，间充质干/祖细胞，单核细胞，骨髓瘤细胞，NK 细胞，其他物种，多能干细胞，T 细胞
种属
人，小鼠，非人灵长类，其他物种，大鼠
应用
冻存
品牌
CryoStor
研究领域
脐带血库，上皮细胞研究，免疫，干细胞生物学
制剂类别
不含动物成分，无血清
实验数据
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (21)
文献 (59)
The immune cell landscape in kidneys of patients with lupus nephritis. Nature immunology 2019
Abstract
Lupus nephritis is a potentially fatal autoimmune disease for which the current treatment is ineffective and often toxic. To develop mechanistic hypotheses of disease, we analyzed kidney samples from patients with lupus nephritis and from healthy control subjects using single-cell RNA sequencing. Our analysis revealed 21 subsets of leukocytes active in disease, including multiple populations of myeloid cells, T cells, natural killer cells and B cells that demonstrated both pro-inflammatory responses and inflammation-resolving responses. We found evidence of local activation of B cells correlated with an age-associated B-cell signature and evidence of progressive stages of monocyte differentiation within the kidney. A clear interferon response was observed in most cells. Two chemokine receptors, CXCR4 and CX3CR1, were broadly expressed, implying a potentially central role in cell trafficking. Gene expression of immune cells in urine and kidney was highly correlated, which would suggest that urine might serve as a surrogate for kidney biopsies.
Super-Obese Patient-Derived iPSC Hypothalamic Neurons Exhibit Obesogenic Signatures and Hormone Responses. Cell stem cell 2018 MAY
Abstract
The hypothalamus contains neurons that integrate hunger and satiety endocrine signals from the periphery and are implicated in the pathophysiology of obesity. The limited availability of human hypothalamic neurons hampers our understanding of obesity disease mechanisms. To address this, we generated human induced pluripotent stem cells (hiPSCs) from multiple normal body mass index (BMI; BMI ≤ 25) subjects and super-obese (OBS) donors (BMI ≥ 50) with polygenic coding variants in obesity-associated genes. We developed a method to reliably differentiate hiPSCs into hypothalamic-like neurons (iHTNs) capable of secreting orexigenic and anorexigenic neuropeptides. Transcriptomic profiling revealed that, although iHTNs maintain a fetal identity, they respond appropriately to metabolic hormones ghrelin and leptin. Notably, OBS iHTNs retained disease signatures and phenotypes of high BMI, exhibiting dysregulated respiratory function, ghrelin-leptin signaling, axonal guidance, glutamate receptors, and endoplasmic reticulum (ER) stress pathways. Thus, human iHTNs provide a powerful platform to study obesity and gene-environment interactions.
Inflammatory Responses and Barrier Function of Endothelial Cells Derived from Human Induced Pluripotent Stem Cells. Stem cell reports 2018 MAY
Abstract
Several studies have reported endothelial cell (EC) derivation from human induced pluripotent stem cells (hiPSCs). However, few have explored their functional properties in depth with respect to line-to-line and batch-to-batch variability and how they relate to primary ECs. We therefore carried out accurate characterization of hiPSC-derived ECs (hiPSC-ECs) from multiple (non-integrating) hiPSC lines and compared them with primary ECs in various functional assays, which included barrier function using real-time impedance spectroscopy with an integrated assay of electric wound healing, endothelia-leukocyte interaction under physiological flow to mimic inflammation and angiogenic responses in in vitro and in vivo assays. Overall, we found many similarities but also some important differences between hiPSC-derived and primary ECs. Assessment of vasculogenic responses in vivo showed little difference between primary ECs and hiPSC-ECs with regard to functional blood vessel formation, which may be important in future regenerative medicine applications requiring vascularization.
更多信息
| 物种 | 人类, 其它物种, 大鼠, 小鼠, 非人灵长类 |
|---|---|
| Contains | • 10% dimethyl sulfoxide (DMSO) • Other ingredients |
| 配方 | 不含动物成分, 无血清 |
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