搜索结果: 'methocult media formulations for human hematopoietic cells serum containing'

EasySep™ Direct人嗜碱性粒细胞分选试剂盒
直接从全血中分离人嗜碱性粒细胞的免疫磁珠负选分离
Ali N et al. (APR 2009) Blood 113 16 3690--5

Forward RNAi screens in primary human hematopoietic stem/progenitor cells.

The mechanisms regulating key fate decisions such as self-renewal and differentiation in hematopoietic stem and progenitor cells (HSPC) remain poorly understood. We report here a screening strategy developed to assess modulators of human hematopoiesis using a lentiviral short hairpin RNA (shRNA) library transduced into cord blood-derived stem/progenitor cells. To screen for modifiers of self-renewal/differentiation,we used the limited persistence of HSPCs under ex vivo culture conditions as a baseline for functional selection of shRNAs conferring enhanced maintenance or expansion of the stem/progenitor potential. This approach enables complex,pooled screens in large numbers of cells. Functional selection identified novel specific gene targets (exostoses 1) or shRNA constructs capable of altering human hematopoietic progenitor differentiation or stem cell expansion,respectively,thereby demonstrating the potential of this forward screening approach in primary human stem cell populations. View Publication

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StemSpan™ SFEM

StemSpan™ SFEM
Bruserud &O et al. (MAY 2003) Leukemia research 27 5 455--64

In vitro culture of human acute lymphoblastic leukemia (ALL) cells in serum-free media; a comparison of native ALL blasts, ALL cell lines and virus-transformed B cell lines.

The aim of this study was to standardize in vitro culture conditions for human acute lymphoblastic leukemia (ALL) cells. The cells were cultured in medium containing 10% fetal calf serum (FCS) and in the four serum-free media X-vivo 10,X-vivo 15,X-vivo 20 and Stem Span. Native ALL blasts could proliferate in all four serum-free media,but the strongest responses were usually observed with Stem Span. Native leukemia blasts were also cultured in the presence of various single cytokines or cytokine combinations. The highest proliferation was usually observed in the presence of Flt3-Ligand (Flt3-L) when single cytokines were examined,and these responses could be further increased especially by combining Flt3-L with interleukin 3 (IL3),IL7 or stem cell factor (SCF). Proliferation could also be increased when ALL blasts were cultured in the presence of two commercially available fibroblast cell lines (Hs27 and HFL1). Based on these results we suggest that in vitro culture conditions for native human ALL blasts can be standardized by using serum-free culture media supplemented with exogenous Flt3-L+IL3+SCF,and the use of accessory cells can also be standardized by using well-characterized fibroblast cell lines. Detectable ALL blast proliferation can then be observed for most patients. Our experimental model can thereby be used for in vitro evaluation of possible antileukemic treatment strategies,and it will then allow comparison of experimental results between different studies. View Publication

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技术手册
Human Colony-Forming Unit (CFU) Assays Using MethoCult™

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MethoCult™ SF H4436

MethoCult™ H4034 Optimum启动试剂盒套装

MethoCult™ H4100

MethoCult™H4230

MethoCult™SF H4236

MethoCult™H4431

MethoCult™H4434经典

MethoCult™H4434经典

MethoCult™ H4434 Classic启动试剂盒套装

MethoCult™H4531

MethoCult™H4535富集无EPO

MethoCult™ H4535 Enriched，不含EPO

MethoCult™ SF H4536

入门套件MethoCult™H4534经典无EPO

含有10% 牛血清白蛋白（BSA）的 Iscove's MDM

MethoCult™H4035 Optimum无EPO

MethoCult™H4330

MethoCult™H4034 Optimum

MethoCult™H4034 Optimum

MethoCult™H4435富集

MethoCult™H4435富集

MethoCult™H4534经典无EPO

MethoCult™H4534经典无EPO

MethoCult™表达

MethoCult™表达

造血祖细胞检测标准化培训课程
EasySep™人单核细胞富集试剂盒（不去除CD16） 免疫磁珠负选未标记的人CD14+CD16+单核细胞
EasySep™人Naïve Pan-T细胞分选试剂盒 免疫磁珠负选未标记的Naïve Pan-T细胞
STEMvision™ 用于造血集落形成单位（CFU）测定的自动化和标准化集落计数
EasySep™ Direct人Naïve B细胞分选试剂盒
直接从全血中分离未标记的初始 B 细胞的免疫磁珠分离
EasySep™人CD4+CD127low CD25+调节性T细胞分选试剂盒 采用可解离磁珠技术进行人CD4+CD127lowCD25+调节性T细胞（Tregs）的免疫磁珠分选
EasySep™人CD4+ CD127low CD49d-调节性T细胞富集试剂盒
对来源于外周血单个核细胞进行免疫磁珠分离，获取未标记的CD4⁺CD127lowCD49d⁻ T 细胞
EasySep™人HLA-DR正选和去除试剂盒
对来源于新鲜或冷冻人外周血单个核细胞或白细胞分离样本中的 HLA-DR⁺ 细胞进行免疫磁珠正选或去除
EasySep™人细胞外囊泡（CD63）正选试剂盒
对来源于血浆、血清、细胞培养上清及尿液的人 CD63⁺ 细胞外囊泡（EVs）进行免疫磁珠正选分离