搜索结果: 'methocult media formulations for human hematopoietic cells serum containing'

EasySep™ Release 人APC正选试剂盒
采用磁珠解离技术，对来源于人外周血单个核细胞或洗涤后白细胞分离样本中标记 APC 偶联抗体的细胞进行免疫磁珠正选分离，分离后的细胞上无磁珠
技术手册
Human Colony-Forming Unit (CFU) Assays Using MethoCult™

产品类型：

产品号#：

00215US.4

04034

04035

04044

04064

04100

04230

04236

04330

04431

04434

04435

04436

04437

04444

04445

04447

04464

04531

04534

04535

04536

04544

04545

04564

09300

产品名：

造血祖细胞检测标准化培训课程

MethoCult™H4034 Optimum

MethoCult™H4035 Optimum无EPO

MethoCult™H4034 Optimum

MethoCult™ H4034 Optimum启动试剂盒套装

MethoCult™ H4100

MethoCult™H4230

MethoCult™SF H4236

MethoCult™H4330

MethoCult™H4431

MethoCult™H4434经典

MethoCult™H4435富集

MethoCult™ SF H4436

MethoCult™表达

MethoCult™H4434经典

MethoCult™H4435富集

MethoCult™表达

MethoCult™ H4434 Classic启动试剂盒套装

MethoCult™H4531

MethoCult™H4534经典无EPO

MethoCult™H4535富集无EPO

MethoCult™ SF H4536

MethoCult™H4534经典无EPO

MethoCult™ H4535 Enriched，不含EPO

入门套件MethoCult™H4534经典无EPO

含有10% 牛血清白蛋白（BSA）的 Iscove's MDM
Bruserud &O et al. (MAY 2003) Leukemia research 27 5 455--64

In vitro culture of human acute lymphoblastic leukemia (ALL) cells in serum-free media; a comparison of native ALL blasts, ALL cell lines and virus-transformed B cell lines.

The aim of this study was to standardize in vitro culture conditions for human acute lymphoblastic leukemia (ALL) cells. The cells were cultured in medium containing 10% fetal calf serum (FCS) and in the four serum-free media X-vivo 10,X-vivo 15,X-vivo 20 and Stem Span. Native ALL blasts could proliferate in all four serum-free media,but the strongest responses were usually observed with Stem Span. Native leukemia blasts were also cultured in the presence of various single cytokines or cytokine combinations. The highest proliferation was usually observed in the presence of Flt3-Ligand (Flt3-L) when single cytokines were examined,and these responses could be further increased especially by combining Flt3-L with interleukin 3 (IL3),IL7 or stem cell factor (SCF). Proliferation could also be increased when ALL blasts were cultured in the presence of two commercially available fibroblast cell lines (Hs27 and HFL1). Based on these results we suggest that in vitro culture conditions for native human ALL blasts can be standardized by using serum-free culture media supplemented with exogenous Flt3-L+IL3+SCF,and the use of accessory cells can also be standardized by using well-characterized fibroblast cell lines. Detectable ALL blast proliferation can then be observed for most patients. Our experimental model can thereby be used for in vitro evaluation of possible antileukemic treatment strategies,and it will then allow comparison of experimental results between different studies. View Publication

产品类型：

产品号#：

产品名：
EasySep™人细胞外囊泡（CD63）正选试剂盒
对来源于血浆、血清、细胞培养上清及尿液的人 CD63⁺ 细胞外囊泡（EVs）进行免疫磁珠正选分离
EasySep™人CD4+ CD127low CD49d-调节性T细胞富集试剂盒
对来源于外周血单个核细胞进行免疫磁珠分离，获取未标记的CD4⁺CD127lowCD49d⁻ T 细胞
EasySep™人HLA-DR正选和去除试剂盒
对来源于新鲜或冷冻人外周血单个核细胞或白细胞分离样本中的 HLA-DR⁺ 细胞进行免疫磁珠正选或去除
EasySep™人单核细胞富集试剂盒（不去除CD16） 免疫磁珠负选未标记的人CD14+CD16+单核细胞
EasySep™人Naïve Pan-T细胞分选试剂盒 免疫磁珠负选未标记的Naïve Pan-T细胞
STEMvision™ 用于造血集落形成单位（CFU）测定的自动化和标准化集落计数
EasySep™ Direct人Naïve B细胞分选试剂盒
直接从全血中分离未标记的初始 B 细胞的免疫磁珠分离
EasySep™人CD4+CD127low CD25+调节性T细胞分选试剂盒 采用可解离磁珠技术进行人CD4+CD127lowCD25+调节性T细胞（Tregs）的免疫磁珠分选
Otsuji TG et al. (MAY 2014) Stem Cell Reports 2 5 734--745

A 3D sphere culture system containing functional polymers for large-scale human pluripotent stem cell production

Utilizing human pluripotent stem cells (hPSCs) in cell-based therapy and drug discovery requires large-scale cell production. However,scaling up conventional adherent cultures presents challenges of maintaining a uniform high quality at low cost. In this regard,suspension cultures are a viable alternative,because they are scalable and do not require adhesion surfaces. 3D culture systems such as bioreactors can be exploited for large-scale production. However,the limitations of current suspension culture methods include spontaneous fusion between cell aggregates and suboptimal passaging methods by dissociation and reaggregation. 3D culture systems that dynamically stir carrier beads or cell aggregates should be refined to reduce shearing forces that damage hPSCs. Here,we report a simple 3D sphere culture system that incorporates mechanical passaging and functional polymers. This setup resolves major problems associated with suspension culture methods and dynamic stirring systems and may be optimal for applications involving large-scale hPSC production. ?? 2014 The Authors. View Publication

产品类型：

产品号#：

85850

85857

产品名：

mTeSR™1

mTeSR™1