搜索结果: 'methocult media formulations for human hematopoietic cells serum containing'

不含EPO的MethoCult™ SF H4636 用于人造血细胞的含重组细胞因子的无血清甲基纤维素培养基
MethoCult™H4534经典无EPO 用于人细胞的含重组细胞因子（不含促红细胞生成素EPO）的甲基纤维素培养基
MethoCult™H4035 Optimum无EPO 含重组细胞因子（不含促红细胞生成素[EPO]）的人细胞甲基纤维素培养基
二甲双胍（Metformin） AMPK 激活剂；线粒体呼吸链复合物 I 抑制剂
产品手册
MethoCult™ Media for Performing Hematopoietic Colony-Forming Unit (CFU) Assays

产品类型：

Cell Culture Media and Supplements

品牌：

MethoCult

产品号#：

03534

03630

03134

03231

03234

03334

03434

03444

03436

04436

04064

04100

04230

04236

04431

04434

04444

04464

04531

04535

04545

04536

04564

07700

07800

07850

27100

27150

38068

28110

28120

28230

28240

产品名：

MethoCult™GF M3534

MethoCult™M3630

MethoCult™M3134

MethoCult™M3231

MethoCult™M3234

MethoCult™M3334

MethoCult™GF M3434

MethoCult™GF M3434

MethoCult™SF M3436

MethoCult™SF H4436

入门套件的MethoCult™H4034优化

MethoCult™H4100

MethoCult™H4230

MethoCult™SF H4236

MethoCult™H4431

MethoCult™H4434经典

MethoCult™H4434经典

入门套件为MethoCult™H4434经典

MethoCult™H4531

MethoCult™H4535富集无EPO

MethoCult™ H4535 Enriched，不含EPO

MethoCult™SF H4536

入门套件MethoCult™H4534经典无EPO

含 2% FBS的Iscove's MDM

氯化铵溶液

氯化铵溶液

35 mm培养皿

35 mm培养皿

Corning® 60 mm 带网格培养皿

16号钝针

钝针

3 mL注射器

3 mL注射器
文献

Lu S-J et al. (JUL 2013) Regenerative medicine 8 4 413--424

3D microcarrier system for efficient differentiation of human pluripotent stem cells into hematopoietic cells without feeders and serum [corrected].

BACKGROUND Human embryonic stem cells (hESCs) have been derived and maintained on mouse embryonic fibroblast feeders to keep their undifferentiated status. To realize their clinical potential,a feeder-free and scalable system for large scale production of hESCs and their differentiated derivatives is required. MATERIALS & METHODS hESCs were cultured and passaged on serum/feeder-free 3D microcarriers for five passages. For embryoid body (EB) formation and hemangioblast differentiation,the medium for 3D microcarriers was directly switched to EB medium. RESULTS hESCs on 3D microcarriers maintained pluripotency and formed EBs,which were ten-times more efficient than hESCs cultured under 2D feeder-free conditions (0.11 ± 0.03 EB cells/hESC input 2D vs 1.19 ± 0.32 EB cells/hESC input 3D). After replating,EB cells from 3D culture readily developed into hemangioblasts with the potential to differentiate into hematopoietic and endothelial cells. Furthermore,this 3D system can also be adapted to human induced pluripotent stem cells,which generate functional hemangioblasts with high efficiency. CONCLUSION This 3D serum- and stromal-free microcarrier system is important for future clinical applications,with the potential of developing to a GMP-compatible scalable system. View Publication

产品类型：

产品号#：

04436

85850

85857

产品名：

MethoCult™SF H4436

mTeSR™1

mTeSR™1
技术公告
MethoCult™ for Rat Hematopoietic Colony-Forming Unit (CFU) Assays

产品类型：

Cell Culture Media and Supplements

细胞类型：

造血干祖细胞

产品号#：

03774

03436

产品名：

MethoCult™GF R3774

MethoCult™SF M3436
Event
iPS Cell Training Course: Reprogramming Somatic Cells to iPS Cells

产品类型：

产品号#：

产品名：
文献

Limó et al. (NOV 1997) Blood 90 9 3316--21

High-titer retroviral vectors containing the enhanced green fluorescent protein gene for efficient expression in hematopoietic cells.

Retroviral vectors constitute the most efficient system to deliver and integrate foreign genes into mammalian cells. We have developed a producer cell line that yields high titers of amphotropic retroviral vectors carrying the enhanced green fluorescent protein (EGFP) gene,a codon humanized,red-shifted variant of the green fluorescent protein (GFP) gene,which can be used as a selectable marker. We have used a hybrid vector that has been shown to efficiently drive gene expression in hematopoietic cells. Virtually all murine and human cell lines and primary human hematopoietic cells tested were transduced with varying efficiency after incubation with vector-containing supernatants. Human CD34(+) cells obtained from cord blood or aphereses products were transduced using a protocol that involves daily addition of vector-containing supernatants for 6 consecutive days. At day 6,up to 16% of the cells expressed EGFP,as assessed by flow cytometry. Sorted EGFP-expressing cells were able to produce fluorescent hematopoietic colonies. EGFP's main advantages are its fast flow cytometry determination and the possibility of cell sorting and simultaneous evaluation of the transduction efficiency along with other phenotypic markers. View Publication

产品类型：

产品号#：

04034

04044

产品名：

MethoCult™H4034 Optimum

MethoCult™H4034 Optimum
文献

Kunova M et al. (NOV 2010) Reproductive biomedicine online 21 5 676--86

Development of humanized culture medium with plant-derived serum replacement for human pluripotent stem cells.

For human embryonic stem cells (ESC) to be used in cell replacement therapies,they must be grown under good manufacturing conditions in a chemically defined medium that lacks animal proteins. This study examined the ability of a newly designed medium containing the plant-derived serum replacement VegetaCell and other reagents of human origin to support undifferentiated growth and pluripotency of human ESC. This medium was tested in several culture systems,using human fibroblasts as a feeder layer or Matrigel in a feeder-free culture. Even under the most stringent feeder-free conditions without conditioned medium,human ESC exhibited an undifferentiated morphology,expressed markers of undifferentiated cells,demonstrated high alkaline phosphatase activity and multilineage differentiation and retained a normal karyotype. Compared with human ESC grown in standard culture conditions,human ESC maintained in humanized VegetaCell medium show longer cell cycles and decreased cell death. The availability of an animal protein-free medium supplemented with the low-cost VegetaCell reagent expands the repertoire of media for culturing human ESC as well as induced pluripotent stem cells for drug testing and cell replacement therapy. View Publication

产品类型：

产品号#：

产品名：
文献

Niwa A et al. (JAN 2011) PLoS ONE 6 7 e22261

A novel Serum-Free monolayer culture for orderly hematopoietic differentiation of human pluripotent cells via mesodermal progenitors

Elucidating the in vitro differentiation of human embryonic stem (ES) and induced pluripotent stem (iPS) cells is important for understanding both normal and pathological hematopoietic development in vivo. For this purpose,a robust and simple hematopoietic differentiation system that can faithfully trace in vivo hematopoiesis is necessary. In this study,we established a novel serum-free monolayer culture that can trace the in vivo hematopoietic pathway from ES/iPS cells to functional definitive blood cells via mesodermal progenitors. Stepwise tuning of exogenous cytokine cocktails induced the hematopoietic mesodermal progenitors via primitive streak cells. These progenitors were then differentiated into various cell lineages depending on the hematopoietic cytokines present. Moreover,single cell deposition assay revealed that common bipotential hemoangiogenic progenitors were induced in our culture. Our system provides a new,robust,and simple method for investigating the mechanisms of mesodermal and hematopoietic differentiation. View Publication

产品类型：

产品号#：

85850

85857

产品名：

mTeSR™1

mTeSR™1
产品手册
StemSpan™: Defined Media and Supplements for Hematopoietic Cell Expansion

产品类型：

Cell Culture Media and Supplements

品牌：

StemSpan

产品号#：

02690

02691

02693

02696

02697

02692

09600

09650

09605

09655

产品名：

StemSpan™CC100

StemSpan™CD34+扩增补充（10X）

StemSpan™髓系扩增补品（100X）

StemSpan™巨核细胞扩增补充（100X）

StemSpan™CC110

StemSpan™红血系扩增补充（100X）

StemSpan™ SFEM

StemSpan™ SFEM

StemSpan™ SFEM II

StemSpan™ SFEM II

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搜索结果: 'methocult media formulations for human hematopoietic cells serum containing'

3D microcarrier system for efficient differentiation of human pluripotent stem cells into hematopoietic cells without feeders and serum [corrected].

High-titer retroviral vectors containing the enhanced green fluorescent protein gene for efficient expression in hematopoietic cells.

Development of humanized culture medium with plant-derived serum replacement for human pluripotent stem cells.

A novel Serum-Free monolayer culture for orderly hematopoietic differentiation of human pluripotent cells via mesodermal progenitors

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