搜索结果: 'gmp t cell activator'

抗人CD3抗体，clone OKT3，FITC 抗人CD3的小鼠Monoclonal IgG2a抗体

抗人CD3抗体，clone UCHT1 小鼠（BALB/c）Monoclonal IgG1抗体，抗人、黑猩猩CD3

抗人CD4，clone RPA-T4 抗人、黑猩猩CD4的小鼠Monoclonal IgG1抗体

EasySep™人CD3正选试剂盒II 人CD3+细胞的免疫磁珠正选试剂盒

抗人CD2抗体，clone RPA-2.10 小鼠抗人、恒河猴、食蟹猴CD2的Monoclonal IgG1抗体

新品

人IgG ELISpot抗体配对试剂盒 用于检测和计数分泌人免疫球蛋白G的细胞

新品

人TNF-α ELISpot试剂盒 用于检测和计数分泌人肿瘤坏死因子-α的细胞

新品

人IL-4 ELISpot试剂盒 用于检测和计数分泌人白介素-4的细胞

新品

人颗粒酶B ELISpot试剂盒 用于检测和计数分泌人颗粒酶B的细胞

Putnam AL et al. (NOV 2013) American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 13 11 3010--20

Clinical grade manufacturing of human alloantigen-reactive regulatory T cells for use in transplantation.

Regulatory T cell (Treg) therapy has the potential to induce transplantation tolerance so that immunosuppression and associated morbidity can be minimized. Alloantigen-reactive Tregs (arTregs) are more effective at preventing graft rejection than polyclonally expanded Tregs (PolyTregs) in murine models. We have developed a manufacturing process to expand human arTregs in short-term cultures using good manufacturing practice-compliant reagents. This process uses CD40L-activated allogeneic B cells to selectively expand arTregs followed by polyclonal restimulation to increase yield. Tregs expanded 100- to 1600-fold were highly alloantigen reactive and expressed the phenotype of stable Tregs. The alloantigen-expanded Tregs had a diverse TCR repertoire. They were more potent than PolyTregs in vitro and more effective at controlling allograft injuries in vivo in a humanized mouse model. View Publication

产品类型：

产品号#：

07930

07931

07940

07955

07956

07959

07954

100-1061

07952

产品名：

CryoStor® CS10

Sá et al. (JUL 2011) Blood 118 4 955--64

Restriction of HIV-1 replication in macrophages and CD4+ T cells from HIV controllers.

How HIV controllers (HICs) maintain undetectable viremia without therapy is unknown. The strong CD8(+) T-cell HIV suppressive capacity found in many,but not all,HICs may contribute to long-lasting viral control. However,other earlier defense mechanisms may be involved. Here,we examined intrinsic HIC cell resistance to HIV-1 infection. After in vitro challenge,monocyte-derived macrophages and anti-CD3-activated CD4(+) T cells from HICs showed low HIV-1 susceptibility. CD4 T-cell resistance was independent of HIV-1 coreceptors and affected also SIVmac infection. CD4(+) T cells from HICs expressed ex vivo higher levels of p21(Waf1/Cip1),which has been involved in the control of HIV-1 replication,than cells from control subjects. However,HIV restriction in anti-CD3-activated CD4(+) T cells and macrophages was not associated with p21 expression. Restriction inhibited accumulation of reverse transcripts,leading to reduction of HIV-1 integrated proviruses. The block could be overcome by high viral inocula,suggesting the action of a saturable mechanism. Importantly,cell-associated HIV-1 DNA load was extremely low in HICs and correlated with CD4(+) T-cell permissiveness to infection. These results point to a contribution of intrinsic cell resistance to the control of infection and the containment of viral reservoir in HICs. View Publication

产品类型：

产品号#：

21000

20119

20155

产品名：

RoboSep™- S

RoboSep™ 吸头组件抛光剂

RoboSep™分选管套装（9个塑料管）

D. M. Previte et al. (apr 2019) Cell reports 27 1 129--141.e4

Lymphocyte Activation Gene-3 Maintains Mitochondrial and Metabolic Quiescence in Naive CD4+ T Cells.

Lymphocyte activation gene-3 (LAG-3) is an inhibitory receptor expressed by CD4+ T cells and tempers their homeostatic expansion. Because CD4+ T cell proliferation is tightly coupled to bioenergetics,we investigate the role of LAG-3 in modulating naive CD4+ T cell metabolism. LAG-3 deficiency enhances the metabolic profile of naive CD4+ T cells by elevating levels of mitochondrial biogenesis. In vivo,LAG-3 blockade partially restores expansion and the metabolic phenotype of wild-type CD4+ T cells to levels of Lag3-/- CD4+ T cells,solidifying that LAG-3 controls these processes. Lag3-/- CD4+ T cells also demonstrate greater signal transducer and activator of transcription 5 (STAT5) activation,enabling resistance to interleukin-7 (IL-7) deprivation. These results implicate this pathway as a target of LAG-3-mediated inhibition. Additionally,enhancement of STAT5 activation,as a result of LAG-3 deficiency,contributes to greater activation potential in these cells. These results identify an additional mode of regulation elicited by LAG-3 in controlling CD4+ T cell responses. View Publication

产品类型：

产品号#：

19852

19852RF

产品名：

EasySep™小鼠CD4+ T细胞分选试剂盒

RoboSep™ 小鼠CD4+ T细胞分选试剂盒

搜索结果: 'gmp t cell activator'

Clinical grade manufacturing of human alloantigen-reactive regulatory T cells for use in transplantation.

Restriction of HIV-1 replication in macrophages and CD4+ T cells from HIV controllers.

Lymphocyte Activation Gene-3 Maintains Mitochondrial and Metabolic Quiescence in Naive CD4+ T Cells.

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