搜索结果: 'methocult media formulations for mouse hematopoietic cells serum containing'

MesenCult™ 脂肪分化试剂盒 (小鼠) 用于小鼠MSCs、ADSCs和MEFs体外向脂肪细胞的分化
MyoCult™ 扩增添加物 10X（小鼠） 小鼠骨骼肌祖细胞（卫星细胞）扩增的含血清补充物
冻存的ACF培养的人外周血来源的未成熟树突状细胞 冻存的人原代细胞
NeuroCult™化学解离试剂盒（小鼠） 小鼠神经球化学解离试剂盒
NeuroCult™ 分化试剂盒（小鼠和大鼠） 用于小鼠和大鼠神经干细胞和祖细胞分化培养的试剂盒
NeuroCult™扩增试剂盒（小鼠和大鼠） 小鼠和大鼠神经干细胞及祖细胞扩增培养基套装
NeuroCult™ 分化添加物（小鼠和大鼠） 小鼠和大鼠神经干祖细胞培养分化添加物
产品手册
MethoCult™ Media for Performing Hematopoietic Colony-Forming Unit (CFU) Assays

产品类型：

品牌：

MethoCult

产品号#：

03134

03231

03234

03334

03434

03436

03444

03534

03630

04064

04100

04230

04236

04431

04434

04436

04444

04464

04531

04535

04536

04545

04564

07700

07800

07850

27100

27150

28110

28120

28230

28240

38068

产品名：

MethoCult™M3134

MethoCult™M3231

MethoCult™M3234

MethoCult™M3334

MethoCult™GF M3434

MethoCult™SF M3436

MethoCult™GF M3434

MethoCult™GF M3534

MethoCult™M3630

MethoCult™ H4034 Optimum启动试剂盒套装

MethoCult™ H4100

MethoCult™H4230

MethoCult™SF H4236

MethoCult™H4431

MethoCult™H4434经典

MethoCult™ SF H4436

MethoCult™H4434经典

MethoCult™ H4434 Classic启动试剂盒套装

MethoCult™H4531

MethoCult™H4535富集无EPO

MethoCult™ SF H4536

MethoCult™ H4535 Enriched，不含EPO

入门套件MethoCult™H4534经典无EPO

含 2% FBS的Iscove's MDM

氯化铵溶液

氯化铵溶液

35 mm培养皿

35 mm培养皿

16号钝针

16号钝针

3 mL注射器

3 mL注射器

Corning® 60 mm 带网格培养皿
技术公告
MethoCult™ Formulation Optimized for Mouse BFU-E Detection

产品类型：

细胞类型：

造血干/祖细胞

产品号#：

03231

03234

03236

03334

03434

03436

03444

产品名：

MethoCult™M3231

MethoCult™M3234

MethoCult™SF M3236

MethoCult™M3334

MethoCult™GF M3434

MethoCult™SF M3436

MethoCult™GF M3434
技术公告
Flow Cytometry Methods for Identifying Mouse Hematopoietic Stem and Progenitor Cells

产品类型：

细胞类型：

造血干/祖细胞

产品号#：

产品名：
Limó et al. (NOV 1997) Blood 90 9 3316--21

High-titer retroviral vectors containing the enhanced green fluorescent protein gene for efficient expression in hematopoietic cells.

Retroviral vectors constitute the most efficient system to deliver and integrate foreign genes into mammalian cells. We have developed a producer cell line that yields high titers of amphotropic retroviral vectors carrying the enhanced green fluorescent protein (EGFP) gene,a codon humanized,red-shifted variant of the green fluorescent protein (GFP) gene,which can be used as a selectable marker. We have used a hybrid vector that has been shown to efficiently drive gene expression in hematopoietic cells. Virtually all murine and human cell lines and primary human hematopoietic cells tested were transduced with varying efficiency after incubation with vector-containing supernatants. Human CD34(+) cells obtained from cord blood or aphereses products were transduced using a protocol that involves daily addition of vector-containing supernatants for 6 consecutive days. At day 6,up to 16% of the cells expressed EGFP,as assessed by flow cytometry. Sorted EGFP-expressing cells were able to produce fluorescent hematopoietic colonies. EGFP's main advantages are its fast flow cytometry determination and the possibility of cell sorting and simultaneous evaluation of the transduction efficiency along with other phenotypic markers. View Publication

产品类型：

产品号#：

04034

04044

产品名：

MethoCult™H4034 Optimum

MethoCult™H4034 Optimum
Lu S-J et al. (JUL 2013) Regenerative medicine 8 4 413--424

3D microcarrier system for efficient differentiation of human pluripotent stem cells into hematopoietic cells without feeders and serum [corrected].

BACKGROUND Human embryonic stem cells (hESCs) have been derived and maintained on mouse embryonic fibroblast feeders to keep their undifferentiated status. To realize their clinical potential,a feeder-free and scalable system for large scale production of hESCs and their differentiated derivatives is required. MATERIALS & METHODS hESCs were cultured and passaged on serum/feeder-free 3D microcarriers for five passages. For embryoid body (EB) formation and hemangioblast differentiation,the medium for 3D microcarriers was directly switched to EB medium. RESULTS hESCs on 3D microcarriers maintained pluripotency and formed EBs,which were ten-times more efficient than hESCs cultured under 2D feeder-free conditions (0.11 ± 0.03 EB cells/hESC input 2D vs 1.19 ± 0.32 EB cells/hESC input 3D). After replating,EB cells from 3D culture readily developed into hemangioblasts with the potential to differentiate into hematopoietic and endothelial cells. Furthermore,this 3D system can also be adapted to human induced pluripotent stem cells,which generate functional hemangioblasts with high efficiency. CONCLUSION This 3D serum- and stromal-free microcarrier system is important for future clinical applications,with the potential of developing to a GMP-compatible scalable system. View Publication

产品类型：

产品号#：

04436

85850

85857

产品名：

MethoCult™ SF H4436

mTeSR™1

mTeSR™1