抗小鼠SSEA-3抗体，克隆号MC-631

靶向人、小鼠、大鼠SSEA-3的大鼠单克隆IgM抗体

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产品号 #60061_C

靶向人、小鼠、大鼠SSEA-3的大鼠单克隆IgM抗体

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总览

实验数据

产品说明书及文档

应用领域

总览

MC-631抗体与阶段特异性胚胎抗原-3 (SSEA-3) 上的半乳糖基球苷表位发生反应，SSEA-3表达于人胚胎癌细胞 (EC)、胚胎生殖细胞 (EG)、未分化胚胎干细胞 (ES) 和诱导多能干细胞 (iPS) 以及恒河猴胚胎干细胞系表面。未分化的鼠胚胎癌细胞、胚胎生殖细胞和胚胎干细胞均未观察到免疫反应。胚胎干细胞和胚胎癌细胞分化后，SSEA-3的表达下调。相反，鼠胚胎干细胞和胚胎癌细胞的分化可能伴随SSEA-3表达的增加。

该抗体克隆已验证可用于标记在 TeSR™-E8™（产品号 #05940）、mTeSR™1（产品号 #85850）和 TeSR™2（产品号 #05860）中生长的人 ES 和 iPS 细胞，并且已验证可用于使用 EasySep™ 试剂盒分选的细胞的纯度评估，包括 EasySep™ 人 ES/iPS 细胞 TRA-1-60 正选试剂盒（产品号 #18166）。

分类
一抗

靶抗原
SSEA-3

别名
阶段特异性胚胎抗原-3

反应种属
人、小鼠、大鼠、恒河猴

偶联
Alexa Fluor 488，PE，未偶联

宿主物种
大鼠

细胞类型
多能干细胞

种属
人，小鼠，非人灵长类，大鼠

应用
ELISAs，流式细胞术，免疫细胞化学，免疫荧光，免疫组化

研究领域
干细胞生物学

克隆
MC-631

Gene ID
93961

Isotype
IgM, κ

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实验数据

Figure 1. Data for Unconjugated

(A) Flow cytometry analysis of human ES cells (filled histogram) or fibroblasts (negative control; dashed line histogram) labeled with Anti-Mouse SSEA-3 Antibody, Clone MC-631, followed by Goat Anti-Rat IgM (Heavy Chain) Antibody, Polyclonal, APC (Catalog #60140AZ). Labeling of human ES cells with a rat IgM isotype control antibody, followed by Goat Anti-Rat IgM (Heavy Chain) Antibody, Polyclonal, APC is shown (solid line histogram). (B) Human ES cells were cultured in mTeSR™1 on Corning® Matrigel®-coated glass slides, then fixed and stained with Anti-Mouse SSEA-3 Antibody, Clone MC-631, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI). Inset shows cells labeled with a rat IgM, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC.

Figure 2. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of human ES cells (filled histogram) or HT1080 fibrosarcoma cells (negative control; dashed line histogram) labeled with AntiMouse SSEA-3 Antibody, Clone MC-631, Alexa Fluor® 488. Labeling of human ES cells with a rat IgM, kappa Alexa Fluor® 488 isotype control antibody is shown (solid line histogram). (B) Human ES cells were cultured in mTeSR™1 on Corning® Matrigel®-coated glass slides, then fixed and stained with Anti-Mouse SSEA-3 Antibody, Clone MC-631, Alexa Fluor® 488. Inset shows cells labeled with a rat IgM, kappa Alexa Fluor® 488 isotype control antibody. (C) Flow cytometry analysis of human iPS cells labeled with Anti-Mouse SSEA-3 Antibody, Clone MC-631, Alexa Fluor® 488 (filled histogram) or a rat IgM, kappa Alexa Fluor® 488 isotype control antibody (solid line histogram).

Figure 3. Data for PE-Conjugated

(A) Flow cytometry analysis of human ES cells (filled histogram) or HT1080 fibrosarcoma cells (negative control; dashed line histogram) labeled with AntiMouse SSEA-3 Antibody, Clone MC-631, PE. Labeling of human ES cells with a rat IgM, kappa PE isotype control antibody is shown (solid line histogram). (B) Human ES cells were cultured in mTeSR™1 on Corning® Matrigel®-coated glass slides, then fixed and stained with Anti-Mouse SSEA-3 Antibody, Clone MC-631, PE. Inset shows cells labeled with a rat IgM, kappa PE isotype control antibody. (C) Flow cytometry analysis of human iPS cells labeled with Anti-Mouse SSEA-3 Antibody, Clone MC-631, PE (filled histogram) or a rat IgM, kappa PE isotype control antibody (solid line histogram).