EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #60060_C
抗人、小鼠、大鼠SSEA-1（CD15）的小鼠单克隆IgM抗体
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总览
MC-480抗体与末端碳水化合物表位，阶段特异性胚胎抗原-1 （SSEA-1）反应，SSEA-1表达于小鼠早期胚胎，小鼠胚胎癌（EC），胚胎干细胞（ES）和小鼠及人胚胎生殖（EG）细胞表面的一种大分子量（> 200 kDa）糖蛋白上。SSEA-1在未分化的人EC、ES或诱导多能干细胞（iPS）或恒河猴ES细胞系上不表达。其在小鼠胚胎干细胞中的表达在分化过程中降低，而在人ES细胞中，其表达在分化过程中会上调。SSEA-1也存在于成人粒细胞和单核细胞中，标记为CD15， MC-480抗体识别这些细胞类型上的CD15标记。据报道，SSEA-1在细胞粘附和迁移以及细胞分化调控中发挥作用。
该抗体Clone已通过EasySep™试剂盒分离的细胞纯度评估验证，包括EasySep™HLA全血CD15正选试剂盒(产品号#18681HLA；可观察到部分阻断)，并用于标记在TeSR™-E8™（产品号#05940），mTeSR™1（产品号#85850）和TeSR™2（产品号#05860）中生长的人 ES和iPS细胞。
分类
一抗
靶抗原
SSEA-1 (CD15)
别名
3-FAL，CD15，Lewis X，SSEA1，阶段特异性胚胎抗原1，X-半抗原
反应种属
人，小鼠，大鼠
偶联
Alexa Fluor 488，生物素，FITC，PE，未偶联
宿主物种
小鼠
细胞类型
多能干细胞
种属
人，小鼠，大鼠
应用
细胞分选，流式细胞术，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀，Western印迹
研究领域
干细胞生物学
Clone
MC-480
Gene ID
14345
Isotype
IgM, κ
实验数据
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (3)
文献 (1)
OCT4 as a target of miR-34a stimulates p63 but inhibits p53 to promote human cell transformation
Cell death & disease 2014 JAN
Abstract
Human cell transformation is a key step for oncogenic development,which involves multiple pathways; however,the mechanism remains unclear. To test our hypothesis whether cell oncogenic transformation shares some mechanisms with the process of reprogramming non-stem cells to induced pluripotent stem cells (iPSC),we studied the relationship among the key factors for promoting or inhibiting iPSC in radiation-transformed human epithelial cell lines derived from different tissues (lung,breast and colon). We unexpectedly found that p63 and OCT4 were highly expressed (accompanied by low expressed p53 and miR-34a) in all transformed cell lines examined when compared with their non-transformed counterparts. We further elucidated the relationship of these factors: the 3p strand of miR-34a directly targeted OCT4 by binding to the 3′ untranslated region (3′-UTR) of OCT4 and,OCT4,in turn,stimulated p63 but inhibited p53 expression by binding to a specific region of the p63 or p53 promoter. Moreover,we revealed that the effects of OCT4 on promoting cell oncogenic transformation were by affecting p63 and p53. These results support that a positive loop exists in human cells: OCT4 upregulation as a consequence of inhibition of miR-34a,promotes p63 but suppresses p53 expression,which further stimulates OCT4 upregulation by downregulating miR-34a. This functional loop contributes significantly to cell transformation and,most likely,also to the iPSC process.
更多信息
| 物种 | 人, 大鼠, 小鼠 |
|---|---|
| 克隆 | MC-480 |
| Gene Id | 14345 |
| Alternative Names | 3-FAL, CD15, Lewis X, SSEA1, Stage-specific embryonic antigen 1, X-hapten |
| 同种型/isotype | IgM, kappa |
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法律声明:
Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
质量保证:
产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE.
Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
质量保证:
产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE.
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