EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #100-0534_C
抑制半胱天冬酶
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总览
Z-VAD-FMK是一种细胞渗透性的合成肽，在体内抑制半胱天冬酶并阻断半胱天冬酶介导的细胞凋亡(Garcia-Calvo等；Xiang et al.)。Z-VAD-FMK可抑制人类胚胎干细胞分化，并提高人类胚胎干细胞在冷冻保存条件下的冻融存活率（Heng et al.）。
癌症研究
·阻断fas诱导的T淋巴细胞凋亡（Chow et al.）。
别名
Z-Val-Ala-Asp(OMe)-氟甲基酮
细胞类型
癌细胞及细胞系，淋巴细胞
研究领域
癌症
CAS 编号
187389-52-2
化学式
C22H30FN3O7
分子量
467.5 g/mol
纯度
≥ 95 %
产品说明书及文档
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相关材料与文献
技术资料 (4)
文献 (4)
Caspase inhibitor Z-VAD-FMK enhances the freeze-thaw survival rate of human embryonic stem cells.
Bioscience reports 2007 oct
Abstract
Previous study demonstrated that the low survival of human embryonic stem cells (hESC) under conventional slow-cooling cryopreservation protocols is predominantly due to apoptosis rather than cellular necrosis. Hence,this study investigated whether a synthetic broad-spectrum irreversible inhibitor of caspase enzymes,Z-VAD-FMK can be used to enhance the post-thaw survival rate of hESC. About 100 mM Z-VAD-FMK was supplemented into either the freezing solution,the post-thaw culture media or both. Intact and adherent hESC colonies were cryopreserved so as to enable subsequent quantitation of the post-thaw cell survival rate through the MTT assay,which can only be performed with adherent cells. Exposure to 100 mM Z-VAD-FMK in the freezing solution alone did not significantly enhance the post-thaw survival rate (10.2{\%} vs. 9.9{\%},p {\textgreater} 0.05). However,when 100 mM Z-VAD-FMK was added to the post-thaw culture media,there was a significant enhancement in the survival rate from 9.9{\%} to 14.4{\%} (p {\textless} 0.05),which was further increased to 18.7{\%} when Z-VAD-FMK was also added to the freezing solution as well (p {\textless} 0.01). Spontaneous differentiation of hESC after cryopreservation was assessed by morphological observations under bright-field microscopy,and by immunocytochemical staining for the pluripotency markers SSEA-3 and TRA-1-81. The results demonstrated that exposure to Z-VAD-FMK did not significantly enhance the spontaneous differentiation of hESC within post-thaw culture.
Involvement of multiple proteases during Fas-mediated apoptosis in T lymphocytes.
FEBS letters 1995 may
Abstract
The mechanism of Fas antigen-mediated apoptosis is at present unclear. We show here that the 100,000 x g supernatant from cell lysates prepared from anti-Fas-stimulated JUR-KAT T cells,induces chromatin fragmentation in isolated nuclei with concomitant morphological changes typically seen in apoptosis. The formation of this apoptotic nuclei promoting activity (ANPA) in JURKAT T cells after Fas antigen ligation was blocked by the serine protease inhibitors,TPCK and DCI,and by the interleukin 1-beta-converting enzyme inhibitor,VAD-FMK. In addition,chromatin degradation and morphological changes mediated by the ANPA in isolated nuclei were inhibited by TPCK,but not by DCI or VAD-FMK. These results suggest that Fas-mediated apoptosis in T cells involves the activation of a cascade of proteases.
BAX-induced cell death may not require interleukin 1 beta-converting enzyme-like proteases.
Proceedings of the National Academy of Sciences of the United States of America 1996 dec
Abstract
Expression of BAX,without another death stimulus,proved sufficient to induce a common pathway of apoptosis. This included the activation of interleukin 1 beta-converting enzyme (ICE)-like proteases with cleavage of the endogenous substrates poly(ADP ribose) polymerase and D4-GDI (GDP dissociation inhibitor for the rho family),as well as the fluorogenic peptide acetyl-Asp-Glu-Val-Asp-aminotrifluoromethylcoumarin (DEVD-AFC). The inhibitor benzyloxycarbonyl-Val-Ala-Asp-fluoromethyl ketone (zVAD-fmk) successfully blocked this protease activity and prevented FAS-induced death but not BAX-induced death. Blocking ICE-like protease activity prevented the cleavage of nuclear and cytosolic substrates and the DNA degradation that followed BAX induction. However,the fall in mitochondrial membrane potential,production of reactive oxygen species,cytoplasmic vacuolation,and plasma membrane permeability that are downstream of BAX still occurred. Thus,BAX-induced alterations in mitochondrial function and subsequent cell death do not apparently require the known ICE-like proteases.
更多信息
| Molecular Weight | 467.5 g/mol |
|---|---|
| Alternative Names | Z-Val-Ala-Asp-(OMe)-fluoromethyl ketone; ZVAD(OMe)-FMK |
| Cas Number | 187389-52-2 |
| Chemical Formula | C22H30FN3O7 |
| 纯度 | ≥ 95 % |
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