STEMprep™ Mouse Brain Dissociation Kit

总览

使用STEMprep™小鼠脑解离试剂盒，高效且可靠地从小鼠脑组织中制备高质量的单细胞悬液。该试剂盒专为与STEMprep™全自动组织处理器配套使用而设计，采用精确配制的酶解液和优化的机械解离方案，最大程度地保持脆弱神经细胞群的完整性和活率。

该方法支持分离多种关键细胞类型，包括小胶质细胞、少突胶质细胞、星形胶质细胞、内皮细胞以及神经干祖细胞。温和的酶消化有助于分解细胞外基质的同时保持细胞结构，减少操作差异和手动操作时间。

解离后获得的样本可直接用于细胞分选、细胞培养、流式细胞术及分子或功能分析等下游应用。

为获得最佳效果，建议将本试剂盒与STEMprep™全自动组织处理器和STEMprep™样本管配套使用。

若需了解更多关于STEMprep™的信息，请访问STEMprep™概览页面。同时，您也可以浏览我们的仪器概览页面或下载我们的仪器服务手册，了解保修范围及其他服务方案。

分类
酶法相关（或酶解类产品）

种属
小鼠

应用
样本制备

品牌
STEMprep

研究领域
癌症, 免疫, 感染性疾病(传染病)

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实验数据

Figure 1. STEMprep™ Mouse Brain Dissociation Kit Achieves High Cell Viability and Yield

Mouse brain tissue was processed into single-cell suspension using the STEMprep™ Mouse Brain Dissociation Kit and the STEMprep Tissue Dissociator, an alternative automated system, or a manual dissociation method. (A) Viability of nucleated cells. (B) Yield of viable cells per whole brain tissue (325 - 395 mg). (C and D) Proportion and yield of CD45+ immune and CD45- non-immune cells, including CD11b+F4/80+ microglia, O4+ oligodendrocytes, ACSA-2+ astrocytes, and CD31+ endothelial cells. Viability, yield, and subset composition were assessed by flow cytometry. Brain samples were processed with protocol-specific density gradient media to remove myelin and cell debris. Red blood cells were lysed with ammonium chloride solution prior to subset analysis. Data are presented as mean ± SD (n = 21 - 36), * p < 0.05, one-way ANOVA with Tukey's multiple comparisons test.

Figure 2. STEMprep™-Processed Mouse Brain Microglia Are Phagocytic and Produce Cytokines upon Activation

Mouse brain tissue was processed into single-cell suspensions using STEMprep™ Mouse Brain Dissociation Kit and the STEMprep™ Tissue Dissociator, an alternative automated system, or a manual dissociation method. (A) Brain CD11b+ microglia were isolated from the single-cell suspensions using EasySep™ Mouse CD11b+ Positive Selection Kit II. (B) The isolated CD11b+ cells were incubated for 2 hours in the presence of pHrodo™ Green-conjugated E. coli BioParticles™ at 2 - 8°C (Cold) or 37°C. The fluorescence of phagocytosed BioParticles™ was measured by flow cytometry. (C) Intracellular flow cytometry staining of TNF-ɑ production by brain CD11b+ microglia cultured overnight in the presence of 3 µg/mL Brefeldin A and treated with (+) or without (-) 100 ng/mL of lipopolysaccharides (LPS). Data are presented as mean ± SD (n = 4).