技术资料

56:31

线上讲座
Modeling Human Gastrointestinal Development and Disease Using Pluripotent Stem Cells

发布日期: 03/06/2017
26:23

播客
Dr. Jason Spence

发布日期: 02/16/2017
15:00

播客
EMBL Organoids 2016 Conference

发布日期: 02/02/2017
6:17

视频
How to Culture Mouse Intestinal Organoids: Passaging

发布日期: 10/09/2015
1:50

视频
Intestinal Organoid Culture: The Next Dimension in Intestinal Research

发布日期: 10/09/2015
11:17

视频
How to Culture Mouse Intestinal Organoids: Isolating Intestinal Crypts and Establishing Organoids

发布日期: 10/08/2015
1:09

视频
How to Culture Mouse Intestinal Organoids: Introduction

发布日期: 10/08/2015
3:19

视频
Why Culture Intestinal Organoids with IntestiCult™ Organoid Growth Medium

发布日期: 01/22/2015
1:03:40

线上讲座
From Stem Cells to Organoids: Modeling the Gastrointestinal Tract

发布日期: 06/20/2014
A. Huber et al. (Jan 2023) STAR protocols 4 102076

Generation of gene-of-interest knockouts in murine organoids using CRISPR-Cas9.

Gene-of-interest knockout organoids present a powerful and versatile research tool to study a gene's effects on many biological and pathological processes. Here,we present a straightforward and broadly applicable protocol to generate gene knockouts in mouse organoids using CRISPR-Cas9 technology. We describe the processes of transient transfecting organoids with pre-assembled CRISPR-Cas9 ribonucleoprotein complexes,organoid cell sorting,and establishing clonal organoid culture pairs. We then detail how to confirm the knockout via Western blot analysis. View Publication

产品号#：

06005

产品名：

IntestiCult™ 类器官生长培养基 (小鼠)
产品手册
qPCR Arrays for Cell Characterization

品牌：

MesenCult
实验方案
How to Generate Human Intestinal Organoid-Derived Monolayers

研究方向：

类器官