技术资料

Keller GM (DEC 1995) Current opinion in cell biology 7 6 862--9

In vitro differentiation of embryonic stem cells.

Under appropriate conditions in culture,embryonic stem cells will differentiate and form embryoid bodies that have been shown to contain cells of the hematopoietic,endothelial,muscle and neuronal lineages. Many aspects of the lineage-specific differentiation programs observed within the embryoid bodies reflect those found in the embryo,indicating that this model system provides access to early cell populations that develop in a normal fashion. Recent studies involving the differentiation of genetically altered embryonic stem cells highlight the potential of this in vitro differentiation system for defining the function of genes in early development. View Publication

产品号#：

06902

06952

00321

00322

00323

00324

00325

产品名：
Limó et al. (NOV 1997) Blood 90 9 3316--21

High-titer retroviral vectors containing the enhanced green fluorescent protein gene for efficient expression in hematopoietic cells.

Retroviral vectors constitute the most efficient system to deliver and integrate foreign genes into mammalian cells. We have developed a producer cell line that yields high titers of amphotropic retroviral vectors carrying the enhanced green fluorescent protein (EGFP) gene,a codon humanized,red-shifted variant of the green fluorescent protein (GFP) gene,which can be used as a selectable marker. We have used a hybrid vector that has been shown to efficiently drive gene expression in hematopoietic cells. Virtually all murine and human cell lines and primary human hematopoietic cells tested were transduced with varying efficiency after incubation with vector-containing supernatants. Human CD34(+) cells obtained from cord blood or aphereses products were transduced using a protocol that involves daily addition of vector-containing supernatants for 6 consecutive days. At day 6,up to 16% of the cells expressed EGFP,as assessed by flow cytometry. Sorted EGFP-expressing cells were able to produce fluorescent hematopoietic colonies. EGFP's main advantages are its fast flow cytometry determination and the possibility of cell sorting and simultaneous evaluation of the transduction efficiency along with other phenotypic markers. View Publication

产品号#：

04034

04044

产品名：

MethoCult™ H4034 Optimum

MethoCult™ H4034 Optimum
产品手册
Services for Cell Therapy by STEMCELL Technologies

品牌：

ImmunoCult
产品手册
Highway1™: Fast, Gentle, and Automated Cell Sorting for Every Lab

品牌：

Highway1
产品手册
Highway1™: High-Purity Sorting of Bacterially-Infected Cells

品牌：

Highway1
技术窍门
Evaluating Hematopoietic Cell Therapy Product Potency Using the CFU Assay
产品手册
人骨髓产品简化您的基于细胞实验的检测流程

品牌：

MesenCult
产品手册
Human CD34+ Cell Isolation Product Selection

品牌：

EasySep
实验方案
Optimizing Delivery Efficiency with Fluorescent Dextran Using the CellPore™ Transfection System

研究方向：

免疫学，疾病建模，药物发现和毒性检测
实验方案
How to Isolate Mouse Bone Marrow Cells by Centrifugation

研究方向：

免疫学
产品手册
造血细胞治疗研究流程

品牌：

ArciTect
产品手册
CellPore™ Mechanoporation Guide

品牌：

CellPore