技术资料

Hale JS et al. (JUN 2010) Journal of immunology (Baltimore,Md. : 1950) 184 11 5964--8

Cutting Edge: Rag deletion in peripheral T cells blocks TCR revision.

Mature CD4(+)Vbeta5(+) T cells that recognize a peripherally expressed endogenous superantigen are tolerized either by deletion or TCR revision. In Vbeta5 transgenic mice,this latter tolerance pathway results in the appearance of CD4(+)Vbeta5(-)TCRbeta(+) T cells,coinciding with Rag1,Rag2,and TdT expression and the accumulation of V(beta)-DJ(beta) recombination intermediates in peripheral CD4(+) T cells. Because postthymic RAG-dependent TCR rearrangement has remained controversial,we sought to definitively determine whether TCR revision is an extrathymic process that occurs in mature peripheral T cells. We show in this study that Rag deletion in post-positive selection T cells in Vbeta5 transgenic mice blocks TCR revision in vivo and that mature peripheral T cells sorted to remove cells bearing endogenous TCRbeta-chains can express newly generated TCRbeta molecules in adoptive hosts. These findings unambiguously demonstrate postthymic,RAG-dependent TCR rearrangement and define TCR revision as a tolerance pathway that targets mature peripheral CD4(+) T cells. View Publication

产品号#：

19751

19751RF

产品名：
Zeng J et al. (MAY 2012) The Journal of Immunology 188 9 4297--4304

Enhancing Immunostimulatory Function of Human Embryonic Stem Cell-Derived Dendritic Cells by CD1d Overexpression

Human embryonic stem cell-derived dendritic cells (hESC-DCs) may potentially provide a platform to generate off-the-shelf" therapeutic cancer vaccines. To apply hESC-DCs for cancer immunotherapy in a semiallogeneic setting� View Publication

产品号#：

05850

05857

05870

05875

09600

09650

70024

70024.1

85850

85857

85870

85875

70025

70025.1

70025.2

70025.3

70047

70047.1

70047.2

70048

70048.1

70048.2

产品名：

StemSpan™ SFEM

StemSpan™ SFEM

冻存的人外周血Pan T细胞

冻存的人外周血Pan T细胞

mTeSR™1

mTeSR™1

冻存的人外周血单个核细胞

冻存的人外周血单个核细胞

冻存的人外周血单个核细胞

冻存的人外周血单个核细胞
科学海报
Generation of T Cells from Hematopoietic Stem and Progenitor Cells in a Stroma-Free, Serum-Free Culture System
Xing J et al. (OCT 2017) Translational oncology 10 5 780--785

BiHC, a T-Cell-Engaging Bispecific Recombinant Antibody, Has Potent Cytotoxic Activity Against Her2 Tumor Cells.

Among different cancer immunotherapy approaches,bispecific antibodies (BsAbs) are of great interest due to their ability to recruit immune cells to kill tumor cells directly. Various BsAbs against Her2 tumor cells have been proposed with potent cytotoxic activities. However,most of these formats require extensive processing to obtain heterodimeric bispecific antibodies. In this study,we describe a bispecific antibody,BiHC (bispecific Her2-CD3 antibody),constructed with a single-domain anti-Her2 and a single-chain Fv (variable fragment) of anti-CD3 in an IgG-like format. In contrast to most IgG-like BsAbs,the two arms in BiHC have different molecular weights,making it easier to separate hetero- or homodimers. BiHC can be expressed in Escherichia coli and purified via Protein A affinity chromatography. The purified BiHC can recruit T cells and induce specific cytotoxicity of Her2-expressing tumor cells in vitro. The BiHC can also efficiently inhibit the tumor growth in vivo. Thus,BiHC is a promising candidate for the treatment of Her2-positive cancers. View Publication

产品号#：

17851

17851RF

100-0692

产品名：

EasySep™人CD3正选试剂盒II

RoboSep™ 人CD3正选试剂盒II

EasySep™人CD3正选试剂盒II
产品手册
高效的单个核细胞分离液

品牌：

Lymphoprep
技术窍门
优化EasySep™ Releasable RapidSpheres™间接正选试剂盒的使用
科学海报
Cell Isolation of Human CD4+ Memory T Cells By Column-Free Immunomagnetic Cell Separation

Conference：

ECI 2006
挂图
Antigen Processing and Presentation Overview of the mechanisms by which antigens are processed and presented to T cells
产品手册
Highway1™: Automated Cell Sorting of PBMCs

品牌：

Highway1
产品手册
Highway1™: Fast Cell Sorting from Bone Marrow

品牌：

Highway1
实验方案
如何使用DNase I减少单细胞悬液中的细胞团块

研究方向：

免疫学，干细胞生物学
实验方案
使用CellPore™细胞转染系统对未激活的T细胞进行基因编辑

研究方向：

免疫学，细胞治疗开发