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RosetteSep™人祖细胞基础预富集试剂盒

免疫密度负选试剂混合物
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产品号 #（选择产品）

产品号 #15226_C

免疫密度负选试剂混合物

产品优势

  • 快捷、操作简单
  • 不需要特殊设备或额外培训
  • 获得的活细胞无标记
  • 可与SepMate™联合使用，实现一致发高通量样本处理

产品组分包括

  • RosetteSep™人祖细胞基础预富集抗体混合物（产品号#15226）          
    • RosetteSep™人祖细胞基础预富集抗体混合物，2mL
  • RosetteSep™人祖细胞基础预富集抗体混合物（产品号#15266）
    • RosetteSep™人祖细胞基础预富集抗体混合物，5x2mL
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要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。
  1. Lymphoprep™
    Lymphoprep™
  2. DPBS（含 2% 胎牛血清）
    DPBS（含 2% 胎牛血清）
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

RosetteSep™人造血祖细胞基础预富集试剂盒专为通过负选法从脐带血中预富集造血祖细胞而设计。非目标细胞通过识别CD3、CD11b、CD19及红细胞上糖蛋白A的抗体复合物被靶向去除。当在Lymphoprep™（产品号#18060）等密度介质上离心时，非目标细胞与红细胞一起沉淀。造血祖细胞作为富集群体出现在血浆与密度介质的交界面处。  

预富集后可使用EasySep™（如EasySep™人CD34正选试剂）从中进一步正选目标细胞。

 

磁极兼容性
 
 
分类
细胞分选试剂盒
 
细胞类型
造血干/祖细胞
 
种属
人
 
样本来源
脐带血
 
分选方法
负选
 
应用
细胞分选
 
品牌
RosetteSep
 
研究领域
免疫，干细胞生物学
 

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实验数据

Typical RosetteSep™ Human Progenitor Cell Basic Preenrichment Profile

Figure 1.Typical RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Profile

Starting with fresh human cord blood, the CD34+CD45+ viable cell content of the pre-enriched fraction typically ranges from 2 - 16%

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Cocktail
Catalog #
15266, 15226
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Cocktail
Catalog #
15266, 15226
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

相关材料与文献

技术资料 (3)

Human Immune Cytokines
Wallchart
Human Immune Cytokines
How to Isolate PBMCs from Whole Blood Using the SepMate™ PBMC Isolation Tubes: 15-Minute Protocol
1:46
Video
How to Isolate PBMCs from Whole Blood Using the SepMate™ PBMC Isolation Tubes: 15-Minute Protocol
Isolate Cells from Whole Blood without Columns or Magnets: RosetteSep™ Immunodensity Cell Separation
2:19
Video
Isolate Cells from Whole Blood without Columns or Magnets: RosetteSep™ Immunodensity Cell Separati...

常见问题

What is RosetteSep™?

RosetteSep™ is a rapid cell separation procedure for the isolation of purified cells directly from whole blood, without columns or magnets.

How does RosetteSep™ work?

The antibody cocktail crosslinks unwanted cells to red blood cells (RBCs), forming rosettes. The unwanted cells then pellet with the free RBCs when centrifuged over a density centrifugation medium (e.g. Ficoll-Paque™ PLUS, Lymphoprep™).

What factors affect cell recovery?

The temperature of the reagents can affect cell recovery. All reagents should be at room temperature (sample, density centrifugation medium, PBS, centrifuge) before performing the isolations. Layering can also affect recovery so be sure to carefully layer the sample to avoid mixing with the density centrifugation medium as much as possible. Be sure to collect the entire enriched culture without disturbing the RBC pellet. A small amount of density centrifugation medium can be collected without worry.

Which cell samples can RosetteSep™ be used with?

RosetteSep™ can be used with leukapheresis samples, bone marrow or buffy coat, as long as: the concentration of cells does not exceed 5 x 107 per mL (can dilute if necessary); and there are at least 100 RBCs for every nucleated cell (RBCs can be added if necessary).

Can RosetteSep™ be used with previously frozen or cultured cells?

Yes. Cells should be re-suspended at 2 - 5 x 107 cells / mL in PBS + 2% FBS. Fresh whole blood should be added at 250 µL per mL of sample, as a source of red cells.

Can RosetteSep™ be used to enrich progenitors from cord blood?

Yes. Sometimes cord blood contains immature nucleated red cells that have a lower density than mature RBCs. These immature red cells do not pellet over Ficoll™, which can lead to a higher RBC contamination than peripheral blood separations.

Does RosetteSep™ work with mouse cells?

No, but we have developed EasySep™, a magnetic-based cell isolation system which works with mouse and other non-human species.

Which anticoagulant should be used with RosetteSep™?

Peripheral blood should be collected in heparinized Vacutainers. Cord blood should be collected in ACD.

Should the anticoagulant be washed off before using RosetteSep™?

No, the antibody cocktail can be added directly to the sample.

更多信息

更多信息
物种 人类
Magnet Compatibility  
样本来源 脐带血
Selection Method Negative
标记抗体
  1. 抗人CD45抗体，clone HI30
    抗人CD45抗体，clone HI30

    小鼠Monoclonal IgG1抗体，抗人、黑猩猩CD45

  2. 抗人CD34抗体，clone 581
    抗人CD34抗体，clone 581

    小鼠Monoclonal IgG1抗体，抗人CD34

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