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RosetteSep™ HLA T细胞富集抗体混合物

免疫密度负选试剂混合物
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产品号 #（选择产品）

产品号 #15061HLA_C

免疫密度负选试剂混合物

产品优势

  • 快捷、操作简单
  • 不需要特殊设备或额外培训
  • 分选得到的细胞未被标记

产品组分包括

  • RosetteSep™ HLA细胞富集抗体混合物（产品号 #15061HLA）
    • RosetteSep™ HLA T细胞富集抗体 混合物，5x2mL
  • RosetteSep™ HLA细胞富集抗体混合物（产品号 #15061HLA）
    • RosetteSep™ HLA T细胞富集抗体 混合物，20x2mL
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要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。
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    Lymphoprep™
  2. DPBS（含 2% 胎牛血清）
    DPBS（含 2% 胎牛血清）
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

RosetteSep™ HLA T细胞富集混合物专为通过负选法从全血中分离T细胞而设计。非目标细胞通过识别非T细胞及红细胞（RBCs）的四聚体抗体复合物（TAC）被靶向去除。当在RosetteSep™ DM-L（产品号#15705）或Lymphoprep™（产品号#18060）等密度梯度介质上离心时，非目标细胞与红细胞一起沉淀。纯化后的T细胞作为高富集群体出现在血浆与密度介质的交界面处。分离出的目标细胞可立即用于血清学或流式细胞术交叉配型检测或其他下游应用。

请注意：根据欧盟议会和理事会关于体外诊断医疗器械的新法规（EU Regulation 2017/746，IVDR），自2022年起生效，STEMCELL已取消这些产品的CE IVD注册声明，现标注为“仅供科研使用”。产品的形式、功能及质量保证均未发生变化。如需更多信息，请联系质量保证与法规部门（qaschangenote@stemcell.com）。

 

子类型
细胞分选试剂盒

细胞类型
T 细胞

物种
人

样本来源
白膜层，全血

分选方式
负选

应用
细胞分选

品牌
RosetteSep

研究领域
HLA，免疫学

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实验数据

Typical RosetteSep™ HLA T Cell Enrichment Profile

Figure 1. Typical RosetteSep™ HLA T Cell Enrichment Profile

Starting with fresh whole blood the CD3+ cell content of the enriched fraction typically ranges from 90% - 97%. Red blood cells were removed by lysis prior to flow cytometry.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet 1
Product Name
RosetteSep™ HLA T Cell Enrichment Cocktail
Catalog #
15061HLA, 15081HLA
Lot #
Lots above 1000081702
Language
English
Document Type
Product Information Sheet 2
Product Name
RosetteSep™ HLA T Cell Enrichment Cocktail
Catalog #
15061HLA, 15081HLA
Lot #
Lots above 1000081702
Language
Multi
Document Type
Product Information Sheet 1
Product Name
RosetteSep™ HLA T Cell Enrichment Cocktail
Catalog #
15081HLA
Lot #
All
Language
English
Document Type
Product Information Sheet 2
Product Name
RosetteSep™ HLA T Cell Enrichment Cocktail
Catalog #
15081HLA
Lot #
All
Language
MULTI
Document Type
Safety Data Sheet
Product Name
RosetteSep™ HLA T Cell Enrichment Cocktail
Catalog #
15061HLA, 15081HLA
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

相关材料与文献

技术资料 (5)

Cell Separation Products for HLA Analysis
Brochure
Cell Separation Products for HLA Analysis
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Isolate Highly Purified Cells for HLA Analysis with RosetteSep™ Immunodensity Cell Isolation
1:21
Video
Isolate Highly Purified Cells for HLA Analysis with RosetteSep™ Immunodensity Cell Isolation
Optimize the Flow Cytometry Crossmatch Assay: Save Time Without Compromising Sensitivity
1:01:28
Webinar
Optimize the Flow Cytometry Crossmatch Assay: Save Time Without Compromising Sensitivity

常见问题

What is RosetteSep™?

RosetteSep™ is a rapid cell separation procedure for the isolation of purified cells directly from whole blood, without columns or magnets.

How does RosetteSep™ work?

The antibody cocktail crosslinks unwanted cells to red blood cells (RBCs), forming rosettes. The unwanted cells then pellet with the free RBCs when centrifuged over a density centrifugation medium (e.g. Ficoll-Paque™ PLUS, Lymphoprep™).

What factors affect cell recovery?

The temperature of the reagents can affect cell recovery. All reagents should be at room temperature (sample, density centrifugation medium, PBS, centrifuge) before performing the isolations. Layering can also affect recovery so be sure to carefully layer the sample to avoid mixing with the density centrifugation medium as much as possible. Be sure to collect the entire enriched culture without disturbing the RBC pellet. A small amount of density centrifugation medium can be collected without worry.

Which cell samples can RosetteSep™ be used with?

RosetteSep™ can be used with leukapheresis samples, bone marrow or buffy coat, as long as: the concentration of cells does not exceed 5 x 107 per mL (can dilute if necessary); and there are at least 100 RBCs for every nucleated cell (RBCs can be added if necessary).

Can RosetteSep™ be used with previously frozen or cultured cells?

Yes. Cells should be re-suspended at 2 - 5 x 107 cells / mL in PBS + 2% FBS. Fresh whole blood should be added at 250 µL per mL of sample, as a source of red cells.

Can RosetteSep™ be used to enrich progenitors from cord blood?

Yes. Sometimes cord blood contains immature nucleated red cells that have a lower density than mature RBCs. These immature red cells do not pellet over Ficoll™, which can lead to a higher RBC contamination than peripheral blood separations.

Does RosetteSep™ work with mouse cells?

No, but we have developed EasySep™, a magnetic-based cell isolation system which works with mouse and other non-human species.

Which anticoagulant should be used with RosetteSep™?

Peripheral blood should be collected in heparinized Vacutainers. Cord blood should be collected in ACD.

Should the anticoagulant be washed off before using RosetteSep™?

No, the antibody cocktail can be added directly to the sample.

文献 (1)

Provision of continuous maturation signaling to dendritic cells by RIG-I-stimulating cytosolic RNA synthesis of Sendai virus. Okano S et al. Journal of immunology (Baltimore, Md. : 1950) 2011 FEB

Abstract

Dendritic cell (DC)-based immunotherapy has potential for treating infections and malignant tumors, but the functional capacity of DC must be assessed in detail, especially maturation and Ag-specific CTL priming. Recent reports suggest that DC that are provided with continuous maturation signals in vivo after transfer into patients are required to elicit the full DC functions. We demonstrate in this study that the rSendai virus vector (SeV) is a novel and ideal stimulant, providing DC with a continuous maturation signal via viral RNA synthesis in the cytosol, resulting in full maturation of monocyte-derived DC(s). Both RIG-I-dependent cytokine production and CD4 T cell responses to SeV-derived helper Ags are indispensable for overcoming regulatory T cell suppression to prime melanoma Ag recognized by T cell-1-specific CTL in the regulatory T cell abundant setting. DC stimulated via cytokine receptors, or TLRs, do not show these functional features. Therefore, SeV-infected DC have the potential for DC-directed immunotherapy.
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更多信息

更多信息
物种 人类
样本来源 全血, 白膜层
Selection Method Negative
标记抗体
  1. 抗人CD3抗体，clone UCHT1
    抗人CD3抗体，clone UCHT1

    小鼠（BALB/c）Monoclonal IgG1抗体，抗人、黑猩猩CD3

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