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RosetteSep™ HLA粒细胞去除抗体混合物

免疫密度去除抗体复合物
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产品号 #（选择产品）

产品号 #15664HLA_C

免疫密度去除抗体复合物

产品优势

  • 快捷、操作简单
  • 不需要特殊设备或额外培训
  • 分选得到的细胞无标记

产品组分包括

  • RosetteSep™HLA     粒细胞去除抗体混合物（产品号 #15664HLA）
  • RosetteSep™ HLA粒细胞去除抗体     混合物，5 x 2 mL
  • RosetteSep™HLA     粒细胞去除抗体混合物（产品号 #15684HLA）
  • RosetteSep™ HLA粒细胞去除抗体     混合物，20 x 2 mL
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  1. Lymphoprep™
    Lymphoprep™
  2. DPBS（含 2% 胎牛血清）
    DPBS（含 2% 胎牛血清）
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

RosetteSep™ HLA 粒细胞去除复合物旨在通过负选从全血中去除粒细胞。非目标细胞经识别 CD66b 与红细胞膜糖蛋白 A 的四聚抗体复合物标记后被去除，经 Lymphoprep™（产品号#18060）离心后，纯化细胞位于血浆与密度介质界面，为无粒细胞的高纯度细胞群。

 

分类
细胞分选试剂盒
 
细胞类型
粒细胞及其亚群
 
种属
人
 
样本来源
白膜层、全血
 
分选方法
去除
 
应用
细胞分选，体外诊断
 
品牌
RosetteSep
 
研究领域
HLA，免疫
 

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实验数据

FACS Results Using RosetteSep™ HLA Granulocyte Depletion Kit

Figure 1. FACS Results Using RosetteSep™ HLA Granulocyte Depletion Kit

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet 1
Product Name
RosetteSep™ HLA Granulocyte Depletion Cocktail
Catalog #
15684HLA, 15664HLA
Lot #
Lots above 1000080451
Language
English
Document Type
Product Information Sheet 2
Product Name
RosetteSep™ HLA Granulocyte Depletion Cocktail
Catalog #
15684HLA, 15664HLA
Lot #
Lots above 1000080451
Language
Multi
Document Type
Safety Data Sheet
Product Name
RosetteSep™ HLA Granulocyte Depletion Cocktail
Catalog #
15684HLA, 15664HLA
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

相关材料与文献

技术资料 (4)

Cell Separation Products for HLA Analysis
Brochure
Cell Separation Products for HLA Analysis
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Isolate Cells from Whole Blood without Columns or Magnets: RosetteSep™ Immunodensity Cell Separation
2:19
Video
Isolate Cells from Whole Blood without Columns or Magnets: RosetteSep™ Immunodensity Cell Separati...
Isolate Highly Purified Cells for HLA Analysis with RosetteSep™ Immunodensity Cell Isolation
1:21
Video
Isolate Highly Purified Cells for HLA Analysis with RosetteSep™ Immunodensity Cell Isolation

常见问题

What is RosetteSep™?

RosetteSep™ is a rapid cell separation procedure for the isolation of purified cells directly from whole blood, without columns or magnets.

How does RosetteSep™ work?

The antibody cocktail crosslinks unwanted cells to red blood cells (RBCs), forming rosettes. The unwanted cells then pellet with the free RBCs when centrifuged over a density centrifugation medium (e.g. Ficoll-Paque™ PLUS, Lymphoprep™).

What factors affect cell recovery?

The temperature of the reagents can affect cell recovery. All reagents should be at room temperature (sample, density centrifugation medium, PBS, centrifuge) before performing the isolations. Layering can also affect recovery so be sure to carefully layer the sample to avoid mixing with the density centrifugation medium as much as possible. Be sure to collect the entire enriched culture without disturbing the RBC pellet. A small amount of density centrifugation medium can be collected without worry.

Which cell samples can RosetteSep™ be used with?

RosetteSep™ can be used with leukapheresis samples, bone marrow or buffy coat, as long as: the concentration of cells does not exceed 5 x 107 per mL (can dilute if necessary); and there are at least 100 RBCs for every nucleated cell (RBCs can be added if necessary).

Can RosetteSep™ be used with previously frozen or cultured cells?

Yes. Cells should be re-suspended at 2 - 5 x 107 cells / mL in PBS + 2% FBS. Fresh whole blood should be added at 250 µL per mL of sample, as a source of red cells.

Can RosetteSep™ be used to enrich progenitors from cord blood?

Yes. Sometimes cord blood contains immature nucleated red cells that have a lower density than mature RBCs. These immature red cells do not pellet over Ficoll™, which can lead to a higher RBC contamination than peripheral blood separations.

Does RosetteSep™ work with mouse cells?

No, but we have developed EasySep™, a magnetic-based cell isolation system which works with mouse and other non-human species.

Which anticoagulant should be used with RosetteSep™?

Peripheral blood should be collected in heparinized Vacutainers. Cord blood should be collected in ACD.

Should the anticoagulant be washed off before using RosetteSep™?

No, the antibody cocktail can be added directly to the sample.

更多信息

更多信息
物种 人类
样本来源 全血, 白膜层
Selection Method Depletion
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