EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #72612_C
腺苷受体、非肌肉肌球蛋白 II (NM II)、MEK1 和极光激酶抑制剂
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总览
逆转素（Reversine）是一种 2,6-二取代的嘌呤衍生物，可抑制人类 A3 腺苷受体、非肌肉肌球蛋白 II 重链、丝裂原激活的细胞外信号调节激酶-1 (MEK1) 和 Aurora B 激酶 (Chen et al.; Perreira et al.; D’Alise et al.)。
重编程
·诱导小鼠成肌细胞去分化为具有成骨和脂肪潜能的多能祖细胞（Chen et al., 2007; Chen et al., 2004)。
·诱导小鼠和人原代真皮成纤维细胞去分化为具有成肌能力的细胞 （Anastasia et al.）。
·诱导纤维环细胞去分化为具有向软骨形成、成骨形成或脂肪形成谱系发育潜力的多能间充质祖细胞（Saraiya et al.）。
癌症研究
·在PC-3、HeLa、CWR22Rv1和DU-145癌细胞系中抑制细胞增殖并诱导多倍体（Hsieh et al.）。
·诱导人胚胎癌细胞系 NT2 和人早幼粒细胞白血病细胞系 HL60 的分化（D’Alise et al.）。
·抑制多种癌细胞系的增殖、导致胞质分裂失败并诱导多倍体化（D’Alise et al.）。
细胞类型
癌细胞及细胞系，白血病/淋巴瘤细胞，间充质干/祖细胞，肌源干/祖细胞
种属
人，小鼠，非人灵长类，其他物种，大鼠
应用
重编程
研究领域
癌症，干细胞生物学
CAS 编号
656820-32-5
化学式
C₂₁H₂₇N₇O
纯度
> 95%
通路
腺苷，极光激酶，MEK/ERK
靶点
腺苷受体，极光激酶B，MEK1，NM II
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (3)
文献 (7)
Dedifferentiation of lineage-committed cells by a small molecule.
Journal of the American Chemical Society 2004 JAN
Abstract
Combinatorial libraries were screened for molecules that induce mouse myogenic lineage committed cells to dedifferentiate in vitro. A 2,6-disubstituted purine,reversine,was discovered that induces lineage reversal of C2C12 cells to become multipotent progenitor cells which can redifferentiate into osteoblasts and adipocytes. This and other such molecules are likely to provide new insights into the molecular mechanisms that control cellular dedifferentiation and may ultimately be useful to in vivo stem cell biology and therapy.
Reversine" and its 2-substituted adenine derivatives as potent and selective A3 adenosine receptor antagonists."
Journal of medicinal chemistry 2005 JUL
Abstract
The dedifferentiation agent reversine" [2-(4-morpholinoanilino)-N(6)-cyclohexyladenine 2] was found to be a moderately potent antagonist for the human A(3) adenosine receptor (AR) with a K(i) value of 0.66 microM. This result prompted an exploration of the structure-activity relationship of related derivatives�
Reversine-treated fibroblasts acquire myogenic competence in vitro and in regenerating skeletal muscle.
Cell death and differentiation 2006 DEC
Abstract
Stem cells hold a great potential for the regeneration of damaged tissues in cardiovascular or musculoskeletal diseases. Unfortunately,problems such as limited availability,control of cell fate,and allograft rejection need to be addressed before therapeutic applications may become feasible. Generation of multipotent progenitors from adult differentiated cells could be a very attractive alternative to the limited in vitro self-renewal of several types of stem cells. In this direction,a recently synthesized unnatural purine,named reversine,has been proposed to induce reversion of adult cells to a multipotent state,which could be then converted into other cell types under appropriate stimuli. Our study suggests that reversine treatment transforms primary murine and human dermal fibroblasts into myogenic-competent cells both in vitro and in vivo. Moreover,this is the first study to demonstrate that plasticity changes arise in primary mouse and human cells following reversine exposure.
更多信息
| 物种 | 人, 其它物种, 大鼠, 小鼠, 非人灵长类 |
|---|---|
| Cas Number | 656820-32-5 |
| Chemical Formula | C₂₁H₂₇N₇O |
| 纯度 | > 95% |
| Target | Adenosine Receptor, Aurora Kinase B, MEK1, NM II |
| Pathway | Adenosine, Aurora Kinase, MEK/ERK |
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