EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #73342_C
抗生素；蛋白质合成抑制剂
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总览
嘌呤霉素是一种氨基核苷类抗生素，源自白黑链霉菌（Streptomyces alboniger），可作为蛋白质合成抑制剂。它与靶核糖体A位点结合，并转移到正在生长的多肽链上，导致多肽链提前终止（Lührmann等；Rodriguez-Fonseca等；Azzam和Algranati）。嘌呤霉素被嘌呤霉素-N-乙酰转移酶N-乙酰化后，会形成一种生物无活性的形式，这使得该基因可用作选择性抗性标记（Vara等，1985）。
细胞系开发
·筛选表达嘌呤霉素-N-乙酰转移酶抗性基因的细胞作为研究工具（de la Luna和Ortín；Iwaki等）。
·可用于CRISPR/Cas9哺乳动物基因编辑，通过筛选成功敲入Cas9基因的嘌呤霉素抗性基因（Park等）。
癌症研究
·经多种细胞系测试，显示其具有抗肿瘤活性（Foley和Eagle）。
细胞类型
癌细胞及细胞系，CHO细胞，杂交瘤细胞，多能干细胞
种属
人，小鼠，非人灵长类，其他物种，大鼠
应用
抗生素
研究领域
癌症，细胞系制备，杂交瘤制备
CAS 编号
58-58-2
化学式
C₂₂H₂₉N₇O₅ · 2HCl
纯度
≥98%
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (1)
文献 (10)
Puromycin-rRNA interaction sites at the peptidyl transferase center.
RNA (New York,N.Y.) 2000 MAY
Abstract
The binding site of puromycin was probed chemically in the peptidyl-transferase center of ribosomes from Escherichia coli and of puromycin-hypersensitive ribosomes from the archaeon Haloferax gibbonsii. Several nucleotides of the 23S rRNAs showed altered chemical reactivities in the presence of puromycin. They include A2439,G2505,and G2553 for E. coli,and G2058,A2503,G2505,and G2553 for Hf. gibbonsii (using the E. coli numbering system). Reproducible enhanced reactivities were also observed at A508 and A1579 within domains I and III,respectively,of E. coli 23S rRNA. In further experiments,puromycin was shown to produce a major reduction in the UV-induced crosslinking of deacylated-(2N3A76)tRNA to U2506 within the P' site of E. coli ribosomes. Moreover,it strongly stimulated the putative UV-induced crosslink between a streptogramin B drug and m2A2503/psi2504 at an adjacent site in E. coli 23S rRNA. These data strongly support the concept that puromycin,along with other peptidyl-transferase antibiotics,in particular the streptogramin B drugs,bind to an RNA structural motif that contains several conserved and accessible base moieties of the peptidyl transferase loop region. This streptogramin motif is also likely to provide binding sites for the 3' termini of the acceptor and donor tRNAs. In contrast,the effects at A508 and A1579,which are located at the exit site of the peptide channel,are likely to be caused by a structural effect transmitted along the peptide channel.
The cytotoxicity of anti-tumor agents for normal human and animal cells in first tissue culture passage.
Cancer research 1958 OCT
Abstract
Rapid selection of Drosophila S2 cells with the puromycin resistance gene.
BioTechniques 2003
Abstract
更多信息
| 物种 | 人, 其它物种, 大鼠, 小鼠, 非人灵长类 |
|---|---|
| Cas Number | 58-58-2 |
| Chemical Formula | C₂₂H₂₉N₇O₅ · 2HCl |
| 纯度 | ≥ 98% |
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