EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #72202_C
Hedgehog 通路激活剂；激活 Smoothened (SMO)
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总览
Purmorphamine 是一种三取代嘌呤衍生物，通过直接结合并激活 Hedgehog 受体 Smoothened (EC₅₀ = 1 µM) 来激活 Hedgehog 通路。（Sinha and Chen）
分化
·促进人多能干细胞向腹侧脊髓祖细胞和运动神经元分化（Hu and Zhang, Karumbayaram et al., Li et al.）。
·促进人和小鼠间充质细胞向成骨细胞分化（Beloti et al., Wu et al. 2002, Wu et al. 2004）。
·抑制人间充质细胞向脂肪细胞分化和成熟（Fontaine et al.）。
细胞类型
间充质干/祖细胞，神经细胞，PSC衍生，成骨细胞，多能干细胞
种属
人，小鼠，非人灵长类，其他物种，大鼠
应用
分化
研究领域
神经科学，干细胞生物学
CAS 编号
483367-10-8
化学式
C₃₁H₃₂N₆O₂
纯度
≥98%
通路
Hedgehog
靶点
SMO
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (4)
文献 (8)
A small molecule with osteogenesis-inducing activity in multipotent mesenchymal progenitor cells.
Journal of the American Chemical Society 2002 DEC
Abstract
Purmorphamine,which is a 2,6,9-trisubstituted purine compound,was discovered through cell-based high-throughput screening from a heterocycle combinatorial library. It differentiates multipotent mesenchymal progenitor cells into an osteoblast lineage. It will serve as a unique chemical tool to study the molecular mechanisms of osteogenesis of stem cells and bone development.
Purmorphamine induces osteogenesis by activation of the hedgehog signaling pathway.
Chemistry & biology 2004 SEP
Abstract
Previously,a small molecule,purmorphamine,was identified that selectively induces osteogenesis in multipotent mesenchymal progenitor cells. In order to gain insights into the mechanism of action of purmorphamine,high-density oligonucleotide microarrays were used to profile gene expression in multipotent mesenchymal progenitor cells treated with either purmorphamine or bone morphogenetic protein-4 (BMP-4). In contrast to BMP-4 treatment,purmorphamine activates the Hedgehog (Hh) signaling pathway,resulting in the up- and downregulation of its downstream target genes,including Gli1 and Patched. Moreover,the known Hh signaling antagonists,cyclopamine and forskolin,completely block the osteogenesis and Glimediated transcription induced by purmorphamine. These results demonstrate that purmorphamine is a small molecule agonist of Hedgehog signaling,and it may ultimately be useful in the treatment of bone-related disease and neurodegenerative disease.
Purmorphamine enhances osteogenic activity of human osteoblasts derived from bone marrow mesenchymal cells.
Cell biology international 2005 JUL
Abstract
Purmorphamine is a novel small molecule with osteogenesis-inducing activity in multipotent mesenchymal progenitor cells,but there has been no evaluation of its effect on human cells to date. The aim of this study was to investigate the induction of osteogenic activity by purmorphamine in human osteoblasts differentiated from bone marrow mesenchymal cells. Cells were cultured in 24-well plates at a density of 2x10(4)/well in medium containing 1,2 or 3 microM purmorphamine,or vehicle. At 7,14 and 21 days,cell proliferation,viability,and alkaline phosphatase (ALP) activity were evaluated. Bone-like nodule formation was evaluated at 21 days. Purmorphamine did not affect cell proliferation or viability,but increased ALP activity and bone-like nodule formation. These results indicate that events related to osteoblast differentiation,including increased ALP activity and bone-like nodule formation,are enhanced by purmorphamine.
更多信息
| 物种 | 人, 其它物种, 大鼠, 小鼠, 非人灵长类 |
|---|---|
| Cas Number | 483367-10-8 |
| Chemical Formula | C₃₁H₃₂N₆O₂ |
| 纯度 | ≥ 98% |
| Target | SMO |
| Pathway | Hedgehog |
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