EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #70025_C
冻存的人原代细胞
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Maximize your savings on high-quality PBMCs! Take advantage of our bulk pricing discounts and price matching offer to get the best value for your purchase. Now available for specific lots, the standard flow cytometry panel provides the exact frequencies of common cell types in each vial of PBMCs. See Figure 1 in the Data Figures section below to view a representative panel. Browse our Frequently Asked Questions for more information.
总览
选择即用型、符合伦理的人原代单个核细胞助您轻松开启实验。我们提供个性化的服务、定制产品、灵活的交付周期，并支持提前测试筛选并预留整个批次，以确保您获得所需的细胞。
通过密度梯度离心法和/或红细胞裂解法从外周血白细胞单采术样本中分离细胞，并冻存于不含动物成分的CryoStor® CS10冻存液（产品号 #07930）中。细胞采集遵循符合伦理规范的方案，并已获得机构审查委员会 (IRB) 的批准，符合美国食品药品监督管理局（FDA）的相关法规和指南；或已获得研究伦理委员会（REC）的批准，并符合英国人体组织管理局（HTA）的相关法规和指南。如有需要，可提供其他文档、高分辨率HLA分型（I类和II类等位基因）以及CMV状态。采集过程中添加酸-柠檬酸-葡萄糖溶液A（ACDA）作为抗凝剂。选择产品选项后，供体信息（如BMI范围、吸烟状态、种族等）可以在上面的评论框中查询。所有供体均经过HIV-1/2、乙肝和丙肝筛查。
某些产品仅在特定地区出售。请与您当地的销售代表或产品与科学支持联系techsupport@stemcell.com获取更多信息。
欲了解更多信息，请浏览有关原代细胞的常见问题解答（FAQs）。
包含
• CryoStor® CS10
分类
冻存
细胞类型
单个核细胞
种属
人
研究领域
药物发现和毒性检测
细胞和组织来源
外周血
供体状态
正常
实验数据
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (12)
文献 (12)
Role for the conserved N-terminal cysteines in the anti-chemokine activities by the chemokine-like protein MC148R1 encoded by Molluscum contagiosum virus.
Virology 2011 SEP
Abstract
Molluscum contagiosum poxvirus (MCV) type 1 and type 2 encode two chemokine-like proteins MC148R1 and MC148R2. It is believed that MC148R proteins function by blocking the inflammatory response. However,the mechanism of the proposed biological activities of MC148R proteins and the role of the additional C-terminal cysteines that do not exist in other chemokines are not understood. Here,we demonstrated in two different assay systems that His-tagged MC148R1 displaces the interaction between CXCL12α and CXCR4. The N-terminal cysteines but not the additional C-terminal cysteines modulate this displacement. His-tagged MC148R1 blocked both CXCL12α-mediated and MIP-1α-mediated chemotaxis. In contrast,MC148R2 blocked MIP-1α-mediated but not CXCL12α-mediated chemotaxis. Immunoprecipitation by antibodies to MC148R1 or CXCL12α followed by immunoblotting and detection by antibodies to the other protein demonstrated physical interaction of His-tagged CXCL12α and His-tagged MC148R1. Interaction with chemokines might mask the receptor interaction site resulting in decreased binding and impairment of the biological activities.
Enhancing Immunostimulatory Function of Human Embryonic Stem Cell-Derived Dendritic Cells by CD1d Overexpression
The Journal of Immunology 2012 MAY
Abstract
Human embryonic stem cell-derived dendritic cells (hESC-DCs) may potentially provide a platform to generate off-the-shelf" therapeutic cancer vaccines. To apply hESC-DCs for cancer immunotherapy in a semiallogeneic setting�
Evaluation of hydrogels for bio-printing applications.
Journal of biomedical materials research. Part A 2013 JAN
Abstract
In the United States alone,there are approximately 500,000 burn injuries that require medical treatment every year. Limitations of current treatments necessitate the development of new methods that can be applied quicker,result in faster wound regeneration,and yield skin that is cosmetically similar to undamaged skin. The development of new hydrogel biomaterials and bioprinting deposition technologies has provided a platform to address this need. Herein we evaluated characteristics of twelve hydrogels to determine their suitability for bioprinting applications. We chose hydrogels that are either commercially available,or are commonly used for research purposes. We evaluated specific hydrogel properties relevant to bioprinting applications,specifically; gelation time,swelling or contraction,stability,biocompatibility and printability. Further,we described regulatory,commercial and financial aspects of each of the hydrogels. While many of the hydrogels screened may exhibit characteristics suitable for other applications,UV-crosslinked Extracel,a hyaluronic acid-based hydrogel,had many of the desired properties for our bioprinting application. Taken together with commercial availability,shelf life,potential for regulatory approval and ease of use,these materials hold the potential to be further developed into fast and effective wound healing treatments.
更多信息
| 物种 | 人 |
|---|---|
| Contains | • CryoStor® CS10 |
| 细胞与组织来源 | 外周血 |
| 捐献者身份 | 正常 |
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质量保证:
产品仅供研究使用，不用于针对人或动物的诊断或治疗。
产品仅供研究使用，不用于针对人或动物的诊断或治疗。
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