Easy 250 EasySep™磁极

大体积免疫磁珠分选磁极

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产品号 #（选择产品）

产品号 #100-0821_C

无柱免疫磁珠分选磁极

产品优势

高效。通过一次分选从大体积样本中分离细胞，提高细胞分选通量。
快速。最快只需20分钟即可从最多225mL的样本中分选免疫细胞。
高纯度。细胞纯度高达99%，且具备高活性和功能性。
灵活。兼容多种类型的EasySep™细胞分选试剂盒以获得您感兴趣的特定细胞类型。

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实验数据

产品说明书及文档

应用领域

总览

轻松高效地扩大手动细胞分选规模并从大体积样本中分选细胞。使用Easy 250 EasySep™磁极，无需分装细胞悬液并进行多轮细胞分选，您可以在单次分选步骤中处理最多225 mL和1.25 x 10¹⁰个细胞。

将Easy 250 EasySep™磁极与标准T-75 cm² 细胞培养瓶和EasySep™ 细胞分选试剂配合使用，无需使用分选柱即可从大体积样本中分离细胞。磁极内部强大的高梯度磁场可分离带有 EasySep™ 磁珠标记的细胞，您只需移取未标记的细胞组分，即可获得可立即用于下游应用的细胞。EasySep™无柱技术可节省时间，并获得高纯度细胞。

不确定使用哪种磁极？访问我们的 EasySep™细胞分选磁极页面比较不同的选择，并为您的研究选择合适的磁极。

了解更多关于 EasySep™免疫磁珠细胞分选技术的工作原理。

种属
人

应用
细胞分选

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EasySep

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实验数据

Figure 1. Using the Easy 250 EasySep™ Magnet Results in the Isolation of Highly Purified Cells of Interest

Human immune cells were isolated from processed leukopaks using the corresponding (A) EasySep™ negative selection kits or (B) EasySep™ positive selection kits (see Table 1). Cell purity was measured before isolation (Start) and after isolation with the Easy 250 EasySep™ Magnet (Easy 250), EasySep™ Magnet (Purple) or “The Big Easy” EasySep™ Magnet (Silver). Purity was assessed by staining with cell surface markers for pan-T cells (CD3+), CD4+ T cells (CD3+CD4+), CD8+ T cells (CD3+CD8+), or monocytes (CD14+CD45+), and analyzed by flow cytometry. Data shown as mean ± SD; n = 4 - 12.

Figure 2. Easy 250 EasySep™ Magnet Can Be Used to Isolate Mononuclear Cells from Whole Blood and Leukopak Samples of Up to 125 mL

Mononuclear cells (MNCs) were isolated using the EasySep™ Direct Human PBMC Isolation Kit (Catalog #19654) from large whole blood samples (25 - 125 mL) or unprocessed leukopaks (25 - 125 mL) with the Easy 250 EasySep™ Magnet (Easy 250) and from small samples (1 mL whole blood) with the EasySep™ Magnet (Purple). (A) Representative flow cytometry plots show the MNC fraction (Glycophorin A- CD42b-). In the above example, the mononuclear cell content of the whole blood start sample (lysed by ammonium chloride) and non-lysed final isolated fraction is 28.9% and 97.1% (not gated on CD45), respectively. (B) Cell purity was measured before (Start) and after isolation (Easy 250, Purple) based on viable cells. Cells were counted and analyzed by flow cytometry. Data shown as mean ± SD; n = 7 - 9.