Easy 250 EasySep™磁极

大体积免疫磁珠分选磁极

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产品号 #（选择产品）

产品号 #100-0821_C

无柱免疫磁珠分选磁极

产品优势

高效。通过一次分选从大体积样本中分离细胞，提高细胞分选通量。
快速。最快只需20分钟即可从最多225mL的样本中分选免疫细胞。
高纯度。细胞纯度高达99%，且具备高活性和功能性。
灵活。兼容多种类型的EasySep™细胞分选试剂盒以获得您感兴趣的特定细胞类型。

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实验数据

产品说明书及文档

应用领域

总览

使用Easy 250 EasySep™磁体，可轻松高效地从大体积样品（如白细胞单采样本或全血）中实现大规模手动细胞分选，而无需将细胞悬液分批进行多次分离。使用该磁体可在单次分离中处理高达225 mL、1.25×10¹⁰个细胞的样本。

配合标准T-75细胞培养瓶和EasySep™试剂盒使用，无需使用分离柱即可从大体积样品中分离细胞。其内部强高梯度磁场可有效分离带有EasySep™磁颗粒标记的细胞，未标记细胞可直接移取用于下游实验。通过EasySep™无柱分选技术，可节省时间并获得高纯度细胞。

访问EasySep™磁体页面以比较不同型号并选择合适磁极。

了解更多关于免疫磁性EasySep™技术的工作原理。

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人

应用
细胞分选

品牌
EasySep

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实验数据

Figure 1. Using the Easy 250 EasySep™ Magnet Results in the Isolation of Highly Purified Cells of Interest

Human immune cells were isolated from processed leukopaks using the corresponding (A) EasySep™ negative selection kits or (B) EasySep™ positive selection kits (see Table 1). Cell purity was measured before isolation (Start) and after isolation with the Easy 250 EasySep™ Magnet (Easy 250), EasySep™ Magnet (Purple) or “The Big Easy” EasySep™ Magnet (Silver). Purity was assessed by staining with cell surface markers for pan-T cells (CD3+), CD4+ T cells (CD3+CD4+), CD8+ T cells (CD3+CD8+), or monocytes (CD14+CD45+), and analyzed by flow cytometry. Data shown as mean ± SD; n = 4 - 12.

Figure 2. Easy 250 EasySep™ Magnet Can Be Used to Isolate Mononuclear Cells from Whole Blood and Leukopak Samples of Up to 125 mL

Mononuclear cells (MNCs) were isolated using the EasySep™ Direct Human PBMC Isolation Kit (Catalog #19654) from large whole blood samples (25 - 125 mL) or unprocessed leukopaks (25 - 125 mL) with the Easy 250 EasySep™ Magnet (Easy 250) and from small samples (1 mL whole blood) with the EasySep™ Magnet (Purple). (A) Representative flow cytometry plots show the MNC fraction (Glycophorin A- CD42b-). In the above example, the mononuclear cell content of the whole blood start sample (lysed by ammonium chloride) and non-lysed final isolated fraction is 28.9% and 97.1% (not gated on CD45), respectively. (B) Cell purity was measured before (Start) and after isolation (Easy 250, Purple) based on viable cells. Cells were counted and analyzed by flow cytometry. Data shown as mean ± SD; n = 7 - 9.