EasySep™小鼠TIL（CD45）正选试剂盒
ClonaCell™用于杂交瘤和细胞系开发
高效细胞克隆与建系专用液体及半固体培养基
利用创新型细胞系专用培养基 ClonaCell™ 系列, 为CHO细胞系、 杂交瘤及其他细胞类型提供最佳培养条件。有甲基纤维素基（半固体）和液体两种形式，ClonaCell™-CHO 和 ClonaCell™-HY 提供甲基纤维素基（半固体）和液体两种配方，仅需一步操作即可实现高单克隆率与克隆多样性。
查看所有 ClonaCell™ 培养基及添加剂, 下载产品手册，选择最适合您需求的产品
使用 ClonaCell™：
- 省时省力，经验证的半固体克隆技术，规避传统有限稀释法的挑战
- 可培养并保藏小鼠、大鼠、人、兔、仓鼠等来源的难养杂交瘤
- 显著提升CHO细胞系开发中的亚克隆效率
多家研究型生物技术公司与抗体服务企业采用ClonaCell™-HY，快速完成抗体开发项目并交付优质抗体
Dr. M. Javad Aman, President and CSO, Integrated BioTherapeutics, Inc.
为何使用 ClonaCell™ ？
- 半固体克隆技术节省时间，避免空孔浪费资源
- 对CHO细胞系、杂交瘤及骨髓瘤具有高的克隆效率与更好的生长支持
- 甲基纤维素基础培养基可根据细胞类型进行定制（可提供配方调整、包装及特殊规格服务）
- 严格性能检测，确保批次间可重复性
- 行业公认的生产工艺与专业的技术支持
立即在您的实验室体验 ClonaCell™ ！
用于杂交瘤生成和扩增的 ClonaCell™ 产品
ClonaCell™-HY 杂交瘤试剂盒
组分（单独提供）：
推荐应用:
小鼠单克隆抗体生产的所有步骤：细胞融合，半固体克隆/杂交瘤挑选和扩增
优势:
- 仅需一步操作即可实现杂交瘤挑选和克隆
- 在半固体培养基中，单细胞来源的杂交瘤可形成清晰独立的克隆集落，便于挑取和筛选
- 避免稀有克隆因过度生长而丢失
ClonaCell™FLEX
规格:
45 mL
形态:
半固体
配方:
- 无血清
- 化学成分明确
- 无动物成份
- 无蛋白
- 甲基纤维素
- 兼容DHFR和GS
推荐应用:
- 开发定制半固体培养基，以用于挑选和克隆悬浮培养的CHO细胞及杂交瘤
- 将液体培养基转化为半固体培养基，用于多种细胞类型的挑选和克隆
- 对已建系悬浮培养的CHO细胞进行再克隆
优势:
- 允许用户制备定制化半固体培养基以支持不同细胞类型
- 允许用户将现有液体克隆体系快速转换为半固体克隆方案
ClonaCell™-CHO CD 液体培养基
规格:
500 mL
形态:
液体
配方:
- DMEM
- 预筛选血
- L-Glutamine
- Gentamycin
- 添加物
- Hypoxanthine
- Aminopterin
- Thymidine
- 2-Mercaptoethanol
推荐应用:
- 细胞融合后杂交瘤的挑选
- 通过有限稀释法克隆单个杂交瘤
优势:
简化液体悬浮培养中杂交瘤的挑选。
资源:
无 HAT 的 ClonaCell™-HY 培养基D
规格:
90 mL
形态:
半固体
配方:
- DMEM
- 预筛选血清
- L-Glutamine
- Gentamyci
- 添加物
- Hypoxanthine
- Aminopterin
- Thymidine
- 2-Mercaptoethanol
推荐应用:
- 杂交瘤一步法筛选与半固体克隆
- 细胞融合后使用
优势:
- 仅需一步操作即可实现杂交瘤挑选和克隆
- 在半固体培养基中，单细胞来源的杂交瘤可形成清晰独立的克隆集落，便于挑取和筛选
- 避免稀有克隆因过度生长而丢失
ClonaCell™-HY AOF 扩增培养基
规格:
500 mL
形态:
液体
配方:
- Hypoxanthine
- Thymidine
- Gentamycin
- Gentamycin
- 重组蛋白
推荐应用:
- 杂交瘤（小鼠、大鼠）和骨髓瘤（小鼠、大鼠、人）的生长和扩增
优势:
- 无动物源
- 减少培养基性能差异
- 简化下游克隆筛选和抗体纯化（无血清源性IgG）
资源:
收起详情
ClonaCell™-HY 杂交瘤试剂盒
ClonaCell™-HY 培养基A
规格:
500 mL
形态:
液体
成份:
- DMEM
- 预筛选血清
- L-Glutamine
- Gentamycin
- 添加物
- 2-Mercaptoethanol
推荐应用:
支持骨髓瘤细胞融合前的生长
ClonaCell™-HY 培养基B
规格:
500 mL
形态:
液体
成份:
- DMEM
- L-Glutamine
- Gentamycin
- 2-Mercaptoethanol
推荐应用:
在细胞融合过程中使用
ClonaCell™-HY 培养基C
规格:
100 mL
形态:
液体
成份:
- DMEM
- 预先确定血清
- L-Glutamine
- Gentamycin
- 添加物
- 2-Mercaptoethanol
推荐应用:
在HAT挑选前促进融合后杂交瘤的活率
ClonaCell™-HY 培养基D
规格:
90 mL
形态:
半固态
成份:
- DMEM
- 预先确定血清
- L-Glutamine
- Gentamycin
- 添加物
- Hypoxanthine
- Aminopterin
- Thymidine
- 2-Mercaptoethanol
推荐应用:
用于杂交瘤的筛选和克隆的一步操作
ClonaCell™-HY 培养基E
规格:
500 mL
形态:
液体
成份:
- DMEM
- 预先确定血清
- L-Glutamine
- Gentamycin
- 添加物
- Hypoxanthine
- Thymidine
- 2-Mercaptoethanol
推荐应用:
在HAT挑选后支持杂交瘤的生长和扩增
收起详情
用于 CHO 细胞系开发的 ClonaCell™
ClonaCell™-CHO CD培养基
规格:
90 mL
形态:
半固态
配方:
- 无血清
- 化学成分明确
- 无动物成份
- 无蛋白
- 兼容DHFR和GS
成份:
- 甲基纤维素
- Kolliphor P188 / Pluronic F68
推荐应用:
- 悬浮培养的CHO细胞的挑选和克隆
- 对已建系悬浮培养的CHO细胞进行再克隆
优势:
在化学成分明确，无动物成分，无蛋白质的培养基中克隆单细胞
ClonaCell™FLEX
规格:
45 mL
格式:
半固态
配方:
- 无血清
- 化学定义
- 动物部分流
- 无蛋白
- DHFR和GS兼容
成份:
甲基纤维素
推荐应用:
- 创建定制的半固体培养基，用于选择和克隆适应悬浮的CHO细胞和杂交瘤
- 将液体培养基转化为半固体培养基，用于多种细胞类型的选择和克隆
- 重建已建立的，适应悬液的CHO细胞
优点:
- 允许用户创建针对各种细胞类型优化的定制半固体培养基
- 允许用户将现有的液体克隆方法转换为半固体克隆方法
ClonaCell™-CHO CD液体培养基
大小:
500 mL
形态:
液体
配方:
- 无血清
- 化学定义
- 动物部分流
- 无蛋白
- DHFR和GS兼容
成份:
Kolliphor P188 / Pluronic F68
推荐应用:
- 悬浮适应型CHO细胞培养
- 适应型CHO细胞的挑选与克隆
- 半固体克隆后扩增CHO细胞
- 搭配ClonaCell™-CHO ACF添加物通过有限稀释法克隆CHO细胞
- 搭配DMSO冻存CHO细胞
优势:
- 支持在化学成分明确，无动物成分，无蛋白质的培养基中扩增CHO细胞
- 与所有 ClonaCell™ 系列产品兼容
ClonaCell™-CHO ACF 培养基
规格:
90 mL
形态:
半固体
配方:
- 无血清
- 无动物成份
- 兼容DHFR和GS
成份:
- 甲基纤维素
- 添加物
- Kolliphor P188 / Pluronic F68
推荐应用:
- 在无血清/无动物成分的体系中挑选和克隆转染的悬浮CHO细胞
- 对已建系悬浮培养的CHO细胞进行再克隆
优势:
- 只含有重组蛋白和合成成分
- 与化学成分明确的无蛋白培养基相比，CHO细胞的单细胞克隆效率更高（特别是在低密度铺板的情况下）
ClonaCell™-CHO ACF 添加物
规格:
2.5 mL
形态:
液体
配方:
- 无血清
- 无动物成份
- 兼容DHFR和GS
成份:
添加物
推荐应用:
- 在单细胞克隆和扩增过程中，添加物化学成分明确的无蛋白培养基或半固体培养基来促进CHO细胞的生长
- 支持 CHO 细胞扩增
优势:
- 仅含有重组蛋白和化学成分的40倍浓缩液
- 不含血清或水解物
ClonaCell™TCS 培养基
规格:
80 mL
形态:
半固态
配方:
血清
成份:
- 甲基纤维素
- 2-Mercaptoethanol
- L-Glutamine
推荐应用:
- 悬浮适应或贴壁CHO细胞及其他细胞系的挑选、克隆和再克隆
- 需要高克隆效率和稳定的集落形成的应用
优势:
支持多种细胞系的高克隆效率和稳定的集落形成，如：CHO-K1， CHO-S, HEK-293, B16F-10, BaF/3, BHK-1, FD-5, Jurkat Daudi, Molt-4, UT-7
资源:
收起详情
科学资源
协议
半固体甲基纤维素细胞培养基的制备与铺板
产品手册
使用EasySep™和ClonaCell™提高杂交瘤实验流程的效率
产品手册
用于杂交瘤和细胞系开发的 ClonaCell™ 培养基
技术窍门
在杂交瘤培养中实现单克隆
Key Applications
Cell Line Development:
Ling SSM et al. (2015) Instrumental Role of Helicobacter Pylori γ-Glutamyl Transpeptidase in VacA-Dependent Vacuolation in Gastric Epithelial Cells. PLoS One 10(6): e0131460.
Rodríguez L et al. (2015) Generation of Monoclonal Antibodies Specific of The Postfusion Conformation of The Pneumovirinae Fusion (F) Protein. J Virol Methods.
Young J et al. (2015) A Novel Immunoassay to Measure Total Serum Lymphotoxin-α Levels in The Presence of An Anti-LTα Therapeutic Antibody. J Immunol Methods Epub ahead.
Chronopoulou E et al. (2014) Hybridoma Technology for The Generation of Rodent mAbs via Classical Fusion. In: Ossipow V, N Fischer (Eds.) Monoclonal Antibodies: Methods and Protocols, Methods in Molecular Biology. 2nd Ed. New York: Springer Science+Business Media.: pp47–70.
Date Y et al. (2014) Label-Free Impedimetric Immunoassay for Trace Levels of Polychlorinated Biphenyls in Insulating Oil. Anal Chem 86(6): 2989–2996.
García-Barreno B et al. (2014) Characterization of An Enhanced Antigenic Change in The Pandemic 2009 H1N1 Influenza Virus Haemagglutinin. J Gen Virol 95(PART 5): 1033–1042.
Tan GS et al. (2014) Characterization of a Broadly Neutralizing Monoclonal Antibody That Targets The Fusion Domain of Group 2 Influenza A Virus Hemagglutinin. J Virol 88(23): 13580–13592.
Kelly-Cirino CD & Mantis NJ. (2009) Neutralizing Monoclonal Antibodies Directed Against Defined Linear Epitopes on Domain 4 of Anthrax Protective Antigen. Infect Immun 77(11): 4859–4867.
Weidanz J a et al. (2006) Levels of Specific Peptide-HLA Class I Complex Predicts Tumor Cell Susceptibility to CTL Killing. J Immunol 177(8): 5088–5097.
Rodríguez L et al. (2015) Generation of Monoclonal Antibodies Specific of The Postfusion Conformation of The Pneumovirinae Fusion (F) Protein. J Virol Methods.
Young J et al. (2015) A Novel Immunoassay to Measure Total Serum Lymphotoxin-α Levels in The Presence of An Anti-LTα Therapeutic Antibody. J Immunol Methods Epub ahead.
Chronopoulou E et al. (2014) Hybridoma Technology for The Generation of Rodent mAbs via Classical Fusion. In: Ossipow V, N Fischer (Eds.) Monoclonal Antibodies: Methods and Protocols, Methods in Molecular Biology. 2nd Ed. New York: Springer Science+Business Media.: pp47–70.
Date Y et al. (2014) Label-Free Impedimetric Immunoassay for Trace Levels of Polychlorinated Biphenyls in Insulating Oil. Anal Chem 86(6): 2989–2996.
García-Barreno B et al. (2014) Characterization of An Enhanced Antigenic Change in The Pandemic 2009 H1N1 Influenza Virus Haemagglutinin. J Gen Virol 95(PART 5): 1033–1042.
Tan GS et al. (2014) Characterization of a Broadly Neutralizing Monoclonal Antibody That Targets The Fusion Domain of Group 2 Influenza A Virus Hemagglutinin. J Virol 88(23): 13580–13592.
Kelly-Cirino CD & Mantis NJ. (2009) Neutralizing Monoclonal Antibodies Directed Against Defined Linear Epitopes on Domain 4 of Anthrax Protective Antigen. Infect Immun 77(11): 4859–4867.
Weidanz J a et al. (2006) Levels of Specific Peptide-HLA Class I Complex Predicts Tumor Cell Susceptibility to CTL Killing. J Immunol 177(8): 5088–5097.
Hybridoma Development with ClonaCell™ in Different Species:
Chronopoulou E et al. (2014) Hybridoma Technology for the Generation of Rodent mAbs via Classical Fusion. In: Ossipow V, N Fischer (Eds.) Monoclonal Antibodies: Methods and Protocols, Methods in Molecular Biology. 2nd Ed. New York: Springer Science+Business Media.: pp47–70.
Cesaro A et al. (2012) An Inflammation Loop Orchestrated by S100A9 and Calprotectin Is Critical for Development of Arthritis. PLoS One 7(9): e45478.
Smith S a. et al. (2012) Persistence of Circulating Memory B Cell Clones with Potential for Dengue Virus Disease Enhancement for Decades following Infection. J Virol 86(5): 2665–2675.
Fang L et al. (2008) Essential Role of TNF Receptor Superfamily 25 (TNFRSF25) in the Development of Allergic Lung Inflammation. J Exp Med 205(5): 1037–1048.
Ulbrandt ND et al. (2006) Isolation and Characterization of Monoclonal Antibodies Which Neutralize Human Metapneumovirus In Vitro and In Vivo. J Virol 80(16): 7799–7806.
Grimaldi JC et al. (1999) Depletion of Eosinophils in Mice through the Use of Antibodies Specific for C-C Chemokine Receptor 3 (CCR3). J Leukoc Biol 65(6): 846–853.
Cesaro A et al. (2012) An Inflammation Loop Orchestrated by S100A9 and Calprotectin Is Critical for Development of Arthritis. PLoS One 7(9): e45478.
Smith S a. et al. (2012) Persistence of Circulating Memory B Cell Clones with Potential for Dengue Virus Disease Enhancement for Decades following Infection. J Virol 86(5): 2665–2675.
Fang L et al. (2008) Essential Role of TNF Receptor Superfamily 25 (TNFRSF25) in the Development of Allergic Lung Inflammation. J Exp Med 205(5): 1037–1048.
Ulbrandt ND et al. (2006) Isolation and Characterization of Monoclonal Antibodies Which Neutralize Human Metapneumovirus In Vitro and In Vivo. J Virol 80(16): 7799–7806.
Grimaldi JC et al. (1999) Depletion of Eosinophils in Mice through the Use of Antibodies Specific for C-C Chemokine Receptor 3 (CCR3). J Leukoc Biol 65(6): 846–853.
Pharmacology and Drug Discovery:
Cabral TM et al. (2014) Development of Neutralizing Monoclonal Antibodies against The Pandemic H1N1 Virus (2009) Using Plasmid DNA Immunogen. J Virol Methods 195: 54–62.
Retamal M et al. (2014) Epitope Mapping of The 2009 Pandemic and the A/Brisbane/59/2007 Seasonal (H1N1) Influenza Virus Haemagglutinins Using mAbs and Escape Mutants. J Gen Virol 95(Pt_11): 2377–2389.
Hostetter DR et al. (2007) Hip Is A Pro-Survival Substrate of Granzyme B. J Biol Chem 282(38): 27865–27874.
Jin C et al. (2006) Immunoglobulin G Specifically Binding Plant N-glycans with High Affinity Could Be Generated in Rabbits But Not in Mice. Glycobiology 16(4): 349–357.
Kuroki M et al. (2005) Preparation of Human IgG and IgM Monoclonal Antibodies for MK-1/Ep-CAM by Using Human Immunoglobulin Gene-Transferred Mouse and Gene Cloning of Their Variable Regions. Anticancer Res 25(6A): 3733–3739.
Retamal M et al. (2014) Epitope Mapping of The 2009 Pandemic and the A/Brisbane/59/2007 Seasonal (H1N1) Influenza Virus Haemagglutinins Using mAbs and Escape Mutants. J Gen Virol 95(Pt_11): 2377–2389.
Hostetter DR et al. (2007) Hip Is A Pro-Survival Substrate of Granzyme B. J Biol Chem 282(38): 27865–27874.
Jin C et al. (2006) Immunoglobulin G Specifically Binding Plant N-glycans with High Affinity Could Be Generated in Rabbits But Not in Mice. Glycobiology 16(4): 349–357.
Kuroki M et al. (2005) Preparation of Human IgG and IgM Monoclonal Antibodies for MK-1/Ep-CAM by Using Human Immunoglobulin Gene-Transferred Mouse and Gene Cloning of Their Variable Regions. Anticancer Res 25(6A): 3733–3739.
Immunology Research:
Flyak AI et al. (2015) Mechanism of Human Antibody-Mediated Neutralization of Marburg Virus. Cell 160(5): 893–903.
Fang L et al. (2008) Essential Role of TNF Receptor Superfamily 25 (TNFRSF25) in The Development of Allergic Lung Inflammation. J Exp Med 205(5): 1037–1048.
Matsumoto SC et al. (2006) Retinal Dysfunction in Patients with Chronic Chagas’ Disease Is Associated to Anti-Trypanosoma Cruzi Antibodies That Cross-React with Rhodopsin. FASEB J 21: 1–21.
Fang L et al. (2008) Essential Role of TNF Receptor Superfamily 25 (TNFRSF25) in The Development of Allergic Lung Inflammation. J Exp Med 205(5): 1037–1048.
Matsumoto SC et al. (2006) Retinal Dysfunction in Patients with Chronic Chagas’ Disease Is Associated to Anti-Trypanosoma Cruzi Antibodies That Cross-React with Rhodopsin. FASEB J 21: 1–21.
Cancer Research:
Chen D et al. (2014) Increased Expression of Id1 and Id3 Promotes Tumorigenicity by Enhancing Angiogenesis and Suppressing Apoptosis in Small Cell Lung Cancer. Genes Cancer 5-6(May): 212–225.
Stern HM et al. (2010) Development of Immunohistochemistry Assays to Assess GALNT14 and FUT3/6 in Clinical Trials of Dulanermin and Drozitumab. Clin Cancer Res 16(5): 1587–1596.
Chen Y et al. (2007) Armed Antibodies Targeting The Mucin Repeats of The Ovarian Cancer Antigen, MUC16, Are Highly Efficacious in Animal Tumor Models. Cancer Res 67(10): 4924–4932.
Wittman VP et al. (2006) Antibody Targeting to A Class I MHC-Peptide Epitope Promotes Tumor Cell Death. J Immunol 177(6): 4187–4195.
Stern HM et al. (2010) Development of Immunohistochemistry Assays to Assess GALNT14 and FUT3/6 in Clinical Trials of Dulanermin and Drozitumab. Clin Cancer Res 16(5): 1587–1596.
Chen Y et al. (2007) Armed Antibodies Targeting The Mucin Repeats of The Ovarian Cancer Antigen, MUC16, Are Highly Efficacious in Animal Tumor Models. Cancer Res 67(10): 4924–4932.
Wittman VP et al. (2006) Antibody Targeting to A Class I MHC-Peptide Epitope Promotes Tumor Cell Death. J Immunol 177(6): 4187–4195.
