ImmunoCult™ 人B细胞扩增试剂盒

扩增B细胞的无血清培养试剂盒

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¥9,654.00

产品号 #（选择产品）

产品号 #100-0645_C

扩增B细胞的无血清培养试剂盒

产品优势

无需使用血清、饲养细胞或专用培养板，即可在体外高效扩增人类B细胞
无血清培养基和无动物源成分添加剂

产品组分包括

ImmunoCult™-XF B细胞基础培养基，100mL
ImmunoCult™-ACF人B细胞扩增添加剂，2mL

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总览

无需使用血清、饲养层细胞或专用培养板，即可实现人B细胞的稳定扩增用于下游检测和应用。

ImmunoCult™人B细胞扩增试剂盒包括ImmunoCult™-ACF人B细胞扩增添加剂和ImmunoCult™-XF B细胞基础培养基，两者共同作用实现人B细胞的持续激活和扩增，并使其成熟为浆细胞。仅需一周或更短时间的培养，即可收获B细胞并直接用于下游应用。为了方便起见，试剂盒中的所有组分也可单独购买。

该试剂盒与我们许多其它上游和下游产品兼容。例如，您可以使用EasySep™细胞分选试剂盒分选各种B细胞亚群-如Pan-B细胞，记忆B细胞和初始B细胞，分选后可立即使用ImmunoCult™人B细胞扩增试剂盒进行扩增培养。

实验数据

Fold Expansion and Maturation of B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Figure 1. Expansion and Maturation of Human B Cells with ImmunoCult™ Human B Cell Expansion Kit

B cells isolated from human peripheral blood mononuclear cells (PBMCs) (leukopak) using EasySep™ Human Pan-B Cell Enrichment Kit (Catalog #19554) were seeded at 1 x 10^5 cells/well in 24-well tissue culture plates with ImmunoCult™-ACF Human B Cell Expansion Supplement and ImmunoCult™-XF B Cell Base Medium included in the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged every 3 - 4 days. (A) Fold expansion of viable cells is shown for n = 12 donors, with bars representing the mean and 95% confidence level (range 38- to 1190-fold at day 14 ± 1 day). (B) Expression of CD138 and CD20 was analyzed by flow cytometry at each timepoint (data represent % positive viable cells; mean ± 1 SD). The observed changes indicate maturation of B cells to plasma cells/blasts.

Microscopy Image of Human B Cells Expanded In Vitro Without the Use of Serum, Feeder Cells, or Specialized Culture Plates

Figure 2. Light Microscopy Image of Cultured Human B Cells

B cells isolated from human PBMCs (leukopak) using EasySep™ Human Pan-B Cell Enrichment Kit were seeded at 1 x 10^5 cells/well in a 24-well tissue culture plate and cultured with the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged on day 4 after seeding and imaged at 40X magnification on day 6.

Figure 3. Expansion and Viability of Human Memory B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Memory B cells were isolated from human PBMCs (leukopak) using EasySep™ Human Memory B Cell Isolation Kit and were seeded at 0.5 x 10^5 cells/well in 48-well tissue culture plates and cultured with the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged every 2 - 4 days and the fold expansion of viable cells (A) and cell viability (B) were calculated at each timepoint. Data represent the mean ± 1 SD of triplicate cultures for the same donor.

Expansion and Viability of Human Naive B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Figure 4. Expansion and Viability of Human Naïve B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Naïve B cells were isolated from human PBMCs (leukopak) using EasySep™ Human Memory B Cell Isolation Kit and were seeded at 0.5 x 10^5 cells/well in 48-well tissue culture plates and cultured with the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged every 2 - 4 days and the fold expansion of viable cells (A) and cell viability (B) were calculated at each timepoint. Data represent the mean ± 1 SD of triplicate cultures for the same donor.

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应用领域

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文献 (1)

Abstract

Background: Human cytomegalovirus (CMV) is a major pathogen that poses significant risks to immunocompromised individuals and neonates. The tegument protein pp71, encoded by the UL82 gene, plays a pivotal role in initiating viral lytic replication and evading host immune responses. Despite its clinical relevance, standardized monoclonal antibodies (mAbs) for pp71 remain limited, prompting the need to expand the available repertoire of antibodies targeting this critical protein. Methods: In this study, we constructed a diverse human single-chain variable fragment (scFv) library using RNA derived from the B cells of four healthy donors. The library was expressed in Saccharomyces cerevisiae , and iterative rounds of magnetic-activated cell sorting (MACS) were performed against recombinant pp71. Clonal enrichment was monitored using flow cytometry. Results: Among the isolated clones, one designated ID2 exhibited high sensitivity and specificity for pp71, as demonstrated by flow cytometry, immunofluorescence, an enzyme-linked immunosorbent assay (ELISA), and biolayer interferometry (BLI). Conclusions: Collectively, these findings establish a novel pp71-specific mAb and underscore the utility of yeast surface display combined with MACS for expanding the antibody toolkit available for CMV research and diagnostics.

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物种	人类
配方	无血清

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物种

人类

配方

无血清

ImmunoCult™ 人B细胞扩增试剂盒

产品号 #100-0645

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产品号 #100-0645_C

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ImmunoCult™ 人B细胞扩增试剂盒

产品号 #100-0645

产品号 #（选择产品）

产品号 #100-0645_C

产品优势

产品组分包括

总览

实验数据

产品说明书及文档

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