In this protocol video, you will learn how to expand human bronchial epithelial cells (HBECs) to initiate an air-liquid interface (ALI) culture using PneumaCult™ culture media.

00:35 Phase 1: Expansion Culture in Flasks
01:37 Plating
02:45 Morphological Assessment
05:36 Phase 2: Submerged Expansion in Inserts

Watch the other videos in this series:
How to Model the Human Airway at the Air-Liquid Interface: Introduction
How to Model the Human Airway at the Air-Liquid Interface: Differentiation of HBECs