In this protocol video, you will learn how to differentiate human bronchial epithelial cells (HBECs) at the air-liquid interface (ALI) using PneumaCult™ culture media.

00: 28 Media preparation
01:46 Air-lifting the culture
03:11 Time course of morphological changes in culture
03:50 Histological staining

Watch the other videos in this series:
How to Model the Human Airway at the Air-Liquid Interface: Introduction
How to Model the Human Airway at the Air-Liquid Interface: Expansion of HBECs