EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #19868_C
免疫磁珠负选试剂盒
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总览
使用 EasySep™ 小鼠上皮细胞富集试剂盒 II，可通过免疫磁性负选法从新鲜解离的小鼠乳腺或其他组织的单细胞悬液中轻松高效地分离高纯度的小鼠上皮细胞。EasySep™ 技术在已发表研究中被广泛使用超过 20 年，结合了单克隆抗体的特异性与无柱磁系统的简便性。
在此 EasySep™ 负选步骤中，不需要的非上皮细胞通过抗体复合物和磁性颗粒进行标记。经 EasySep™ 磁体分离后，带有磁性标记的非上皮细胞被去除，未标记的目标上皮细胞通过倾倒或移液方式转移至新试管中。整个磁性细胞分离过程可在 1 小时内完成，目标上皮细胞可直接用于流式细胞术、培养或 DNA/RNA 提取等下游应用。
了解更多关于 EasySep™ 免疫磁性技术的工作原理。探索更多优化实验流程的产品，包括培养基、补充物、抗体等。
磁极兼容性
• EasySep™磁极（产品号 #18000）
• “The Big Easy” EasySep™磁极（产品号 #18001）
分类
细胞分选试剂盒
细胞类型
乳腺细胞，前列腺细胞
种属
小鼠
样本来源
其他物种，原代细胞
分选方法
负选
应用
细胞分选
品牌
EasySep
研究领域
上皮细胞研究
实验数据
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (4)
文献 (2)
Sterol regulatory element binding protein 1 couples mechanical cues and lipid metabolism. Nature communications 2019
Abstract
Sterol regulatory element binding proteins (SREBPs) are a family of transcription factors that regulate lipid biosynthesis and adipogenesis by controlling the expression of several enzymes required for cholesterol, fatty acid, triacylglycerol and phospholipid synthesis. In vertebrates, SREBP activation is mainly controlled by a complex and well-characterized feedback mechanism mediated by cholesterol, a crucial bio-product of the SREBP-activated mevalonate pathway. In this work, we identified acto-myosin contractility and mechanical forces imposed by the extracellular matrix (ECM) as SREBP1 regulators. SREBP1 control by mechanical cues depends on geranylgeranyl pyrophosphate, another key bio-product of the mevalonate pathway, and impacts on stem cell fate in mouse and on fat storage in Drosophila. Mechanistically, we show that activation of AMP-activated protein kinase (AMPK) by ECM stiffening and geranylgeranylated RhoA-dependent acto-myosin contraction inhibits SREBP1 activation. Our results unveil an unpredicted and evolutionary conserved role of SREBP1 in rewiring cell metabolism in response to mechanical cues.
NF-κB non-cell-autonomously regulates cancer stem cell populations in the basal-like breast cancer subtype. Nature communications 2013
Abstract
Patients with triple-negative breast cancer display the highest rates of early relapse of all patients with breast cancer. The basal-like subtype, a subgroup of triple-negative breast cancer, exhibits high levels of constitutively active NF-κB signalling. Here we show that NF-κB activation, induced by inflammatory cytokines or by epigenetically dysregulated NIK expression, cell-autonomously upregulates JAG1 expression in non-cancer stem cells. This upregulation stimulates NOTCH signalling in cancer stem cells in trans, leading to an expansion of cancer stem cell populations. Among breast cancers, the NF-κB-dependent induction of JAG1 and the NOTCH-dependent expansion of the cancer stem cell population occur only in the basal-like subtype. Collectively, our results indicate that NF-κB has a non-cell-autonomous role in regulating cancer stem cell populations by forming intratumoural microenvironments composed of JAG1-expressing non-cancer stem cells with a basal-like subtype.
更多信息
| 物种 | 小鼠 |
|---|---|
| Magnet Compatibility | • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) |
| 样本来源 | 其它细胞系, 原代细胞 |
| Selection Method | Negative |
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