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EasySep™人CD11b正选和去除试剂盒

对来源于外周血单个核细胞或白细胞分离样本的人 CD11b⁺ 细胞进行免疫磁珠正选或负选分离

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产品号 #（选择产品）

产品号 #100-0742_C

免疫磁正选与细胞去除试剂盒

产品优势

  • 易于操作、快速
  • 纯度高达99%
  • 无需分离柱

产品组分包括

  • EasySep™人CD11b正选和去除试剂盒（产品号 #100-0742）
    • EasySep™人CD11b正选去除抗体混合物，0.75 mL
    • EasySep™ Dextran RapidSpheres™ 50100 磁珠，2 x 1 mL
  • RoboSep™人CD11b阳性分选与去除试剂盒(产品号#100-0744)
    • EasySep™人CD11b正选去除抗体混合物，0.75 mL
    • EasySep™ Dextran RapidSpheres™ 50100 磁珠，2 x 1 mL
    • RoboSep™ 缓冲液（产品号 #20104）
    • RoboSep™过滤吸头（产品号 #21000）
立即询价
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

使用EasySep™人CD11b正选或去除试剂盒，通过免疫磁性正选法，可轻松从新鲜或冻存的人外周血单个核细胞（PBMCs）或白细胞单采样品中分离或去除CD11b+细胞。EasySep™技术在已发表研究中应用超过20年，结合单克隆抗体的特异性与无柱磁分离系统的简便性。在此正选步骤中，目标细胞被识别CD11b的抗体复合物和磁性颗粒标记。

标记的细胞通过EasySep™磁体分离，未标记的细胞被去除，CD11b+细胞保留在试管中。整个分离过程短至16分钟，分离得到的细胞可直接用于流式细胞术、培养或DNA/RNA提取。该试剂盒靶向人CD11b，是整合素Mac-1抗原受体的亚基，常用作髓系细胞标志物。CD11b表达于单核细胞、巨噬细胞、中性粒细胞及NK细胞一些亚群，参与调控细胞黏附、迁移及吞噬作用。

了解更多关于免疫磁性EasySep™技术的工作原理，或了解如何使用RoboSep™系统实现免疫磁性细胞分离自动化。探索更多产品，包括培养基、添加剂、抗体等，为您的实验流程提供优化方案。

 

磁极兼容性
• EasySep™磁极（产品号 #18000）
• “The Big Easy” EasySep™磁极（产品号 #18001）
• Easy 50 EasySep™磁极（产品号 #18002）
• EasyEights™ EasySep™磁极（产品号 #18103）
• RoboSep™-S（产品号 #21000）
 
分类
细胞分选试剂盒
 
细胞类型
粒细胞及其亚群，巨噬细胞，单核细胞，NK 细胞
 
种属
人
 
样本来源
白细胞单采术样本，外周血
 
分选方法
去除，正选
 
应用
细胞分选
 
品牌
EasySep，RoboSep
 
研究领域
免疫，感染性疾病(传染病)
 

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实验数据

Typical EasySep™ Human CD11b+ Positive Selection Profile

Figure 1. Typical EasySep™ Human CD11b+ Positive Selection Profile

Starting with human PBMCs, the CD11b+ cell content of the isolated fraction is typically 98.6 ± 1.4% after positive selection and 1.7 ± 1.2% after depletion (mean ± SD using the EasySep™ magnet). In the above example, the frequencies of CD11b+ cells in the start, final isolated, and final depleted fractions are 47.8%, 99.4%, and 1.6%, respectively.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
RoboSep™ Human CD11b Positive Selection and Depletion Kit
Catalog #
100-0744
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
EasySep™ Human CD11b Positive Selection and Depletion Kit
Catalog #
100-0742
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Human CD11b Positive Selection and Depletion Kit
Catalog #
100-0744
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Human CD11b Positive Selection and Depletion Kit
Catalog #
100-0744
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Human CD11b Positive Selection and Depletion Kit
Catalog #
100-0744
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human CD11b Positive Selection and Depletion Kit
Catalog #
100-0742
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human CD11b Positive Selection and Depletion Kit
Catalog #
100-0742
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

相关材料与文献

技术资料 (7)

Human Monocyte Research Product Workflow
Brochure
Human Monocyte Research Product Workflow
Tools to Streamline your Dendritic Cell Research
Brochure
Tools to Streamline your Dendritic Cell Research
Isolate Human Immune Cells
Brochure
Isolate Human Immune Cells
Tools to Streamline your Macrophage Research
Brochure
Tools to Streamline your Macrophage Research
Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation

文献 (1)

Antibody-Mediated LILRB2-Receptor Antagonism Induces Human Myeloid-Derived Suppressor Cells to Kill Mycobacterium tuberculosis. V. K. Singh et al. Frontiers in immunology 2022

Abstract

Tuberculosis is a leading cause of death in mankind due to infectious agents, and Mycobacterium tuberculosis (Mtb) infects and survives in macrophages (MФs). Although MФs are a major niche, myeloid-derived suppressor cells (MDSCs) are an alternative site for pathogen persistence. Both MФs and MDSCs express varying levels of leukocyte immunoglobulin-like receptor B (LILRB), which regulate the myeloid cell suppressive function. Herein, we demonstrate that antagonism of LILRB2 by a monoclonal antibody (mab) induced a switch of human MDSCs towards an M1-macrophage phenotype, increasing the killing of intracellular Mtb. Mab-mediated antagonism of LILRB2 alone and its combination with a pharmacological blockade of SHP1/2 phosphatase increased proinflammatory cytokine responses and phosphorylation of ERK1/2, p38 MAPK, and NF-kB in Mtb-infected MDSCs. LILRB2 antagonism also upregulated anti-mycobacterial iNOS gene expression and an increase in both nitric oxide and reactive oxygen species synthesis. Because genes associated with the anti-mycobacterial function of M1-MФs were enhanced in MDSCs following mab treatment, we propose that LILRB2 antagonism reprograms MDSCs from an immunosuppressive state towards a pro-inflammatory phenotype that kills Mtb. LILRB2 is therefore a novel therapeutic target for eradicating Mtb in MDSCs.
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更多信息

更多信息
物种 人类
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • Easy 50 EasySep™ Magnet (Catalog #18002) • RoboSep™-S (Catalog #21000)
样本来源 外周血, 白细胞单采术样本
Selection Method Depletion, Positive
标记抗体
  1. 抗人CD45抗体，clone HI30
    抗人CD45抗体，clone HI30

    小鼠Monoclonal IgG1抗体，抗人、黑猩猩CD45

  2. 抗人CD11b抗体，clone ICRF44
    抗人CD11b抗体，clone ICRF44

    抗人、恒河猴、食蟹猴CD11b的小鼠Monoclonal IgG1抗体

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