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用于可视化和优化CellPore™细胞转染系统胞内递送效率的荧光示踪剂
若您需要咨询产品或有任何技术问题,请通过官方电话 400 885 9050 或邮箱 info.cn@stemcell.com 与我们联系。
使用CellPore™ FITC-Dextran(一种用于评估转染效率的绿色荧光示踪剂),并结合CellPore™细胞转染系统优化基于机械穿孔的递送流程。CellPore™ FITC-Dextran采用即用型设计,可作为所有应用的便捷、通用的阳性对照物质。
这种亲水性多糖由荧光素异硫氰酸酯(FITC)标记的葡聚糖组成,具有低毒性和低免疫原性的特点,可实现可靠的下游分析,且对细胞健康的影响极小。
在机械穿孔过程中,瞬时的膜通透性使FITC-Dextran能够快速进入细胞,与内吞作用的自然摄取相比,能够产生更加显著的胞内荧光。使用CellPore™ FITC-Dextran作为阳性对照,可确定适用于您细胞类型和应用的最佳CellPore™递送压强。完成初始优化运行后,选择能够最大程度提高细胞摄取率并保持细胞活率的压力,以用于后续实验。CellPore™ FITC-Dextran还可以与其他功能性物质共递送,以便在进行功能性检测的同时追踪转染效率。递送性能可通过流式细胞术进行分析,并且该标记物与多色流式染色板兼容。
为获得最佳效果,请与CellPore™细胞转染系统、CellPore™递送管和CellPore™递送液配合使用。
有关CellPore™的更多信息,包括CellPore™细胞转染系统中使用的胞内递送技术,请访问CellPore™总览页面。
细胞类型
造血干祖细胞、单核细胞、NK细胞、多能干细胞、T细胞、CD4+ T细胞、CD8+ T细胞
物种
人、小鼠
应用
基因组编辑
品牌
CellPore™
研究方向
疾病建模、药物发现和毒性测试、免疫学
请在《产品说明书》中查找相关支持信息和使用说明,或浏览下方更多实验方案。
| 物种 | 人类, 小鼠 |
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Legal Statement:
The purchase of a CellPore™ Transfection System, CellPore™ Delivery Cartridges, and associated reagents (“CellPore™ Products”) conveys to the purchaser a limited, non-exclusive, non-transferable license to use CellPore™ Products, in accordance with STEMCELL's Terms and Conditions of Sale and all applicable laws and regulations, and solely for research-use-only applications (which applications exclude any commercial application or any therapeutic, prophylactic, diagnostic application, as well as any development and/or commercialization of products therewith or developed therefrom). CellPore™, SQZ™, Cell Squeeze™, and Empower Cells to Change Lives™ are trademarks of STEMCELL Technologies Canada Inc., following its acquisition of SQZ Biotech. Nothing in this agreement grants the purchaser any rights to use these trademarks without prior written permission from STEMCELL. STEMCELL or its licensor(s) grant no additional license rights other than those explicitly granted herein, and except for such limited license rights, all other intellectual property and proprietary rights in and to CellPore™ Products are and shall remain the exclusive property of STEMCELL and its licensors. The purchaser of CellPore™ Products agrees to prevent unauthorized use, access, copying, or disclosure of any intellectual property comprised in CellPore™ Products. The purchaser shall not, and shall not permit anyone to, copy, create any derivative work, reverse engineer, disassemble, decompile, or make improvements to CellPore™ products. Users and purchasers of CellPore™ products hereby grant to STEMCELL exclusive ownership and all rights, title, and interest in and to any improvements made, by purchasers or users, to the CellPore™ products (including any improvements by purchasers or users to the intellectual property directly related to CellPore™ products), provided however that the user or purchaser who makes the improvement retains a limited, non-transferable license to use such improvement for its internal, non-commercial research use, in accordance with this agreement, only so long as such user or purchaser is in compliance with this agreement. All data, information, and results (to the extent they do not constitute improvements of CellPore™ products) entered, stored, compiled, generated, and/or analyzed by purchasers of CellPore™ products using the CellPore™ products in accordance with all of the foregoing shall be the property of such purchaser. CellPore™ Transfection System and CellPore™ Delivery Cartridges (“CellPore™ Products”) are protected by patents in the U.S. and elsewhere. More information can be found at www.cellpore.com. The list of products and patents contained therein are subject to change as a result of routine prosecution and dependent on the patent life cycle as determined by the laws of each jurisdiction in which a relevant patent is in effect.
质量声明:
产品仅供科研使用,除非另有说明,不适用于人体或动物的诊断或治疗用途。欲获悉更多关于STEMCELL的质控信息,请访问STEMCELL.CN/COMPLIANCE。
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