搜索结果: 'EasySep Release'

Korpi-Steiner NL et al. (DEC 2006) Journal of leukocyte biology 80 6 1364--74

Human rhinovirus induces robust IP-10 release by monocytic cells, which is independent of viral replication but linked to type I interferon receptor ligation and STAT1 activation.

Human rhinovirus (HRV)-induced respiratory infections are associated with elevated levels of IFN-gamma-inducible protein 10 (IP-10),which is an enhancer of T lymphocyte chemotaxis and correlates with symptom severity and T lymphocyte number. Increased IP-10 expression is exhibited by airway epithelial cells following ex vivo HRV challenge and requires intracellular viral replication; however,there are conflicting reports regarding the necessity of type I IFN receptor ligation for IP-10 expression. Furthermore,the involvement of resident airway immune cells,predominantly bronchoalveolar macrophages,in contributing to HRV-stimulated IP-10 elaboration remains unclear. In this regard,our findings demonstrate that ex vivo exposure of human peripheral blood monocytes and bronchoalveolar macrophages (monocytic cells) to native or replication-defective HRV serotype 16 (HRV16) resulted in similarly robust levels of IP-10 release,which occurred in a time- and dose-dependent manner. Furthermore,HRV16 induced a significant increase in type I IFN (IFN-alpha) release and STAT1 phosphorylation in monocytes. Neutralization of the type I IFN receptor and inhibition of JAK or p38 kinase activity strongly attenuated HRV16-stimulated STAT1 phosphorylation and IP-10 release. Thus,this work supports a model,wherein HRV16-induced IP-10 release by monocytic cells is modulated via autocrine/paracrine action of type I IFNs and subsequent JAK/STAT pathway activity. Our findings demonstrating robust activation of monocytic cells in response to native and/or replication-defective HRV16 challenge represent the first evidence indicating a mechanistic disparity in the activation of macrophages when compared with epithelial cells and suggest that macrophages likely contribute to cytokine elaboration following HRV challenge in vivo. View Publication

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RosetteSep™人单核细胞富集抗体混合物

RosetteSep™人单核细胞富集抗体混合物
新闻
STEMCELL Technologies Announces FDA De Novo Classification for Its EasySep™ CD138 Positive Selection Kit to Support Cancer Diagnostic Tests

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发布日期: 03/26/2024
科学海报
Optimized Capture and Purification of Nucleic Acid Using the EasySep™ Total Nucleic Acid Extraction Kit, A Robust Magnetic Particle-Based Extraction System

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人全血CD34+细胞分选完全试剂盒 免疫磁珠正选
技术窍门
使‌用‌‌EasySep™‌‌紫‌色‌磁‌极‌和‌‌EasyPlate™‌‌磁‌极‌对‌小‌体‌积‌样‌本‌或‌低‌细‌胞‌数‌量‌样‌本‌进‌行‌分‌选‌

发布日期: 04/30/2013
技术窍门
优化EasySep™ Releasable RapidSpheres™间接正选试剂盒的使用
产品手册
EasySep™细胞分选技术 - 更高效地分离细胞

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EasySep

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EasySep™人CD4+CD127low CD25+调节性T细胞分选试剂盒

EasySep™人CD3正选试剂盒II

RoboSep™ 人CD3正选试剂盒II

EasySep™人CD4正选试剂盒II

RoboSep™ 人CD4正选试剂盒II

EasySep™人CD8正选试剂盒 II

RoboSep™ 人CD8正选试剂盒 II

EasySep™人CD19正选试剂盒II

RoboSep™ 人CD19正选试剂盒II

EasySep™人CD56正选试剂盒 II

RoboSep™ 人CD56正选试剂盒 II

EasySep™人CD14正选试剂盒II

RoboSep™ 人CD14正选试剂盒II

EasySep™人脐带血CD34正选试剂盒II

RoboSep™ 人脐带血CD34正选试剂盒II

EasySep™人T细胞分选试剂盒

RoboSep™ 人T细胞分选试剂盒

EasySep™人CD4+ T细胞分选试剂盒

RoboSep™ 人CD4+ T细胞分选试剂盒

EasySep™人CD8+ T细胞分选试剂盒

RoboSep™ 人CD8+ T细胞分选试剂盒
技术窍门
EasySep™细胞分选详细指南：技巧与提示
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EasySep™ Direct 仅需20分钟，从全血中分选细胞

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EasySep™ Direct人Naïve B细胞分选试剂盒

EasySep™ Direct人总淋巴细胞分选试剂盒

EasySep™ Direct人T细胞分选试剂盒

EasySep™ Direct人CD4+ T细胞分选试剂盒

RoboSep™ Direct人CD4+ T细胞分选试剂盒

EasySep™ Direct人CD8+ T细胞分选试剂盒

EasySep™ Direct人B-CLL细胞分选试剂盒

EasySep™ Direct人中性粒细胞分选试剂盒

EasySep™ Direct人单核细胞分选试剂盒

EasySep™ Direct人B细胞分选试剂盒

RoboSep™ Direct人B细胞分选试剂盒

EasySep™ Direct人NK细胞分选试剂盒

EasySep™ Direct人CTC富集试剂盒

EasySep™ Direct人Pan-粒细胞分选试剂盒

RoboSep™ Direct人总淋巴细胞分选试剂盒
技术窍门
使用EasySep™ 负选试剂盒获得更高的细胞回收率

发布日期: 03/25/2011
Littlewood-Evans A et al. (AUG 2016) The Journal of experimental medicine

GPR91 senses extracellular succinate released from inflammatory macrophages and exacerbates rheumatoid arthritis.

When SUCNR1/GPR91-expressing macrophages are activated by inflammatory signals,they change their metabolism and accumulate succinate. In this study,we show that during this activation,macrophages release succinate into the extracellular milieu. They simultaneously up-regulate GPR91,which functions as an autocrine and paracrine sensor for extracellular succinate to enhance IL-1β production. GPR91-deficient mice lack this metabolic sensor and show reduced macrophage activation and production of IL-1β during antigen-induced arthritis. Succinate is abundant in synovial fluids from rheumatoid arthritis (RA) patients,and these fluids elicit IL-1β release from macrophages in a GPR91-dependent manner. Together,we reveal a GPR91/succinate-dependent feed-forward loop of macrophage activation and propose GPR91 antagonists as novel therapeutic principles to treat RA. View Publication

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19861

19861RF

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EasySep™小鼠单核细胞分选试剂盒

RoboSep™ 小鼠单核细胞分选试剂盒
M. Stoszko et al. (aug 2020) Science advances 6 33 eaba6617

Gliotoxin, identified from a screen of fungal metabolites, disrupts 7SK snRNP, releases P-TEFb, and reverses HIV-1 latency.

A leading pharmacological strategy toward HIV cure requires shock" or activation of HIV gene expression in latently infected cells with latency reversal agents (LRAs) followed by their subsequent clearance. In a screen for novel LRAs we used fungal secondary metabolites as a source of bioactive molecules. Using orthogonal mass spectrometry (MS) coupled to latency reversal bioassays we identified gliotoxin (GTX) as a novel LRA. GTX significantly induced HIV-1 gene expression in latent ex vivo infected primary cells and in CD4+ T cells from all aviremic HIV-1+ participants. RNA sequencing identified 7SK RNA the scaffold of the positive transcription elongation factor b (P-TEFb) inhibitory 7SK small nuclear ribonucleoprotein (snRNP) complex to be significantly reduced upon GTX treatment of CD4+ T cells. GTX directly disrupted 7SK snRNP by targeting La-related protein 7 (LARP7) releasing active P-TEFb which phosphorylated RNA polymerase II (Pol II) C-terminal domain (CTD) inducing HIV transcription." View Publication

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15022

15062

产品名：

RosetteSep™人CD4+ T细胞富集抗体混合物

RosetteSep™人CD4+ T细胞富集抗体混合物