EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #72402_C
非肌肉肌球蛋白 II (NM II) ATPase 抑制剂
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总览
(-)-Blebbistatin是是一种选择性细胞通透性非肌肉型肌球蛋白 II ATPase 抑制剂 (Kovács et al.; Straight et al.)。它能快速且可逆地抑制多个物种的 Mg-ATPase 活性和非肌肉型肌球蛋白 IIA 和 IIB 的体外运动能力 (IC₅₀ = 0.5-5.0 μM)，而对平滑肌肌球蛋白的抑制作用较弱 (IC₅₀ = 80 μM) （Limouze et al.）。
维持和自我更新
·添加ROCK抑制剂后提高人多能干细胞（hPSC）解离为单细胞后的存活率和克隆效率（Chen et al.; Ohgushi et al.; Walker et al.; Xu et al.)。
·使 hPSC 能够在无表面涂层的微载体上培养（Chen et al.）。
·抑制人间充质干细胞的分化（McBeath et al.; Engler et al.)。
细胞类型
间充质干/祖细胞，多能干细胞
种属
人，小鼠，非人灵长类，其他物种，大鼠
应用
培养
研究领域
干细胞生物学
CAS 编号
856925-71-8
化学式
C₁₈H₁₆N₂O₂
纯度
≥98%
靶点
NM II ATPase
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
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相关材料与文献
技术资料 (3)
文献 (10)
Dissecting temporal and spatial control of cytokinesis with a myosin II Inhibitor.
Science 2003 MAR
Abstract
Completion of cell division during cytokinesis requires temporally and spatially regulated communication from the microtubule cytoskeleton to the actin cytoskeleton and the cell membrane. We identified a specific inhibitor of nonmuscle myosin II,blebbistatin,that inhibited contraction of the cleavage furrow without disrupting mitosis or contractile ring assembly. Using blebbistatin and other drugs,we showed that exit from the cytokinetic phase of the cell cycle depends on ubiquitin-mediated proteolysis. Continuous signals from microtubules are required to maintain the position of the cleavage furrow,and these signals control the localization of myosin II independently of other furrow components.
Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment.
Developmental cell 2004 APR
Abstract
Commitment of stem cells to different lineages is regulated by many cues in the local tissue microenvironment. Here we demonstrate that cell shape regulates commitment of human mesenchymal stem cells (hMSCs) to adipocyte or osteoblast fate. hMSCs allowed to adhere,flatten,and spread underwent osteogenesis,while unspread,round cells became adipocytes. Cell shape regulated the switch in lineage commitment by modulating endogenous RhoA activity. Expressing dominant-negative RhoA committed hMSCs to become adipocytes,while constitutively active RhoA caused osteogenesis. However,the RhoA-mediated adipogenesis or osteogenesis was conditional on a round or spread shape,respectively,while constitutive activation of the RhoA effector,ROCK,induced osteogenesis independent of cell shape. This RhoA-ROCK commitment signal required actin-myosin-generated tension. These studies demonstrate that mechanical cues experienced in developmental and adult contexts,embodied by cell shape,cytoskeletal tension,and RhoA signaling,are integral to the commitment of stem cell fate.
Mechanism of blebbistatin inhibition of myosin II.
The Journal of biological chemistry 2004 AUG
Abstract
Blebbistatin is a recently discovered small molecule inhibitor showing high affinity and selectivity toward myosin II. Here we report a detailed investigation of its mechanism of inhibition. Blebbistatin does not compete with nucleotide binding to the skeletal muscle myosin subfragment-1. The inhibitor preferentially binds to the ATPase intermediate with ADP and phosphate bound at the active site,and it slows down phosphate release. Blebbistatin interferes neither with binding of myosin to actin nor with ATP-induced actomyosin dissociation. Instead,it blocks the myosin heads in a products complex with low actin affinity. Blind docking molecular simulations indicate that the productive blebbistatin-binding site of the myosin head is within the aqueous cavity between the nucleotide pocket and the cleft of the actin-binding interface. The property that blebbistatin blocks myosin II in an actin-detached state makes the compound useful both in muscle physiology and in exploring the cellular function of cytoplasmic myosin II isoforms,whereas the stabilization of a specific myosin intermediate confers a great potential in structural studies.
更多信息
| 物种 | 人, 其它物种, 大鼠, 小鼠, 非人灵长类 |
|---|---|
| Cas Number | 856925-71-8 |
| Chemical Formula | C₁₈H₁₆N₂O₂ |
| 纯度 | ≥ 98% |
| Target | NM II ATPase |
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