EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #60062_C
靶向人、小鼠、大鼠SSEA-4的小鼠单克隆IgG3抗体
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总览
MC-813-70 抗体与阶段特异性胚胎抗原-4 (SSEA-4) 发生反应，SSEA-4 是一种糖脂类碳水化合物抗原，表达于人胚胎癌细胞 (EC)、胚胎生殖细胞 (EG)、未分化胚胎干细胞 (ES) 和诱导多能干细胞 (iPS) 表面，以及部分间充质干细胞和恒河猴胚胎干细胞系。未分化的小鼠 EC、EG、ES 和 iPS 细胞均未观察到免疫反应。人 EC、ES 和 iPS 细胞分化后，SSEA-4 的表达下调。相反，小鼠 EC、ES 或 iPS 细胞的分化可能伴随 SSEA-4 表达的增加。
该抗体克隆已验证可用于标记在 mTeSR™1（产品号 #85850）和 TeSR™2（产品号 #05860）中生长的人 ES 和 iPS 细胞，并且已验证可用于使用 EasySep™ 试剂盒分选的细胞的纯度评估，包括 EasySep™ 人 ES/iPS 细胞 TRA-1-60 正选试剂盒（产品号 #18166）和 EasySep™ hESC/hiPSC SSEA-4 正选试剂盒（产品号 #18165；可能会观察到部分阻断）。
分类
一抗
靶抗原
SSEA-4
别名
阶段特异性胚胎抗原-4
反应种属
猫、鸡、犬、人、小鼠、兔、大鼠、恒河猴
偶联
Alexa Fluor 488，生物素，FITC，PE，未偶联
宿主物种
小鼠
细胞类型
多能干细胞
种属
人，小鼠，非人灵长类，其他物种，大鼠
应用
细胞分选，ELISAs，流式细胞术，免疫细胞化学，免疫荧光，免疫组化
研究领域
干细胞生物学
克隆
MC-813-70
Gene ID
330401
Isotype
IgG3, κ轻链
实验数据
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
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相关材料与文献
技术资料 (3)
文献 (6)
Intrinsic Immunity Shapes Viral Resistance of Stem Cells. Cell 2018 JAN
Abstract
Stem cells are highly resistant to viral infection compared to their differentiated progeny; however, the mechanism is mysterious. Here, we analyzed gene expression in mammalian stem cells and cells at various stages of differentiation. We find that, conserved across species, stem cells express a subset of genes previously classified as interferon (IFN) stimulated genes (ISGs) but that expression is intrinsic, as stem cells are refractory to interferon. This intrinsic ISG expression varies in a cell-type-specific manner, and many ISGs decrease upon differentiation, at which time cells become IFN responsive, allowing induction of a broad spectrum of ISGs by IFN signaling. Importantly, we show that intrinsically expressed ISGs protect stem cells against viral infection. We demonstrate the in vivo importance of intrinsic ISG expression for protecting stem cells and their differentiation potential during viral infection. These findings have intriguing implications for understanding stem cell biology and the evolution of pathogen resistance.
Simple suspension culture system of human iPS cells maintaining their pluripotency for cardiac cell sheet engineering. Journal of Tissue Engineering and Regenerative Medicine 2015 DEC
Abstract
In this study, a simple three-dimensional (3D) suspension culture method for the expansion and cardiac differentiation of human induced pluripotent stem cells (hiPSCs) is reported. The culture methods were easily adapted from two-dimensional (2D) to 3D culture without any additional manipulations. When hiPSCs were directly applied to 3D culture from 2D in a single-cell suspension, only a few aggregated cells were observed. However, after 3 days, culture of the small hiPSC aggregates in a spinner flask at the optimal agitation rate created aggregates which were capable of cell passages from the single-cell suspension. Cell numbers increased to approximately 10-fold after 12 days of culture. The undifferentiated state of expanded hiPSCs was confirmed by flow cytometry, immunocytochemistry and quantitative RT-PCR, and the hiPSCs differentiated into three germ layers. When the hiPSCs were subsequently cultured in a flask using cardiac differentiation medium, expression of cardiac cell-specific genes and beating cardiomyocytes were observed. Furthermore, the culture of hiPSCs on Matrigel-coated dishes with serum-free medium containing activin A, BMP4 and FGF-2 enabled it to generate robust spontaneous beating cardiomyocytes and these cells expressed several cardiac cell-related genes, including HCN4, MLC-2a and MLC-2v. This suggests that the expanded hiPSCs might maintain the potential to differentiate into several types of cardiomyocytes, including pacemakers. Moreover, when cardiac cell sheets were fabricated using differentiated cardiomyocytes, they beat spontaneously and synchronously, indicating electrically communicative tissue. This simple culture system might enable the generation of sufficient amounts of beating cardiomyocytes for use in cardiac regenerative medicine and tissue engineering.
OCT4 as a target of miR-34a stimulates p63 but inhibits p53 to promote human cell transformation Cell death & disease 2014 JAN
Abstract
Human cell transformation is a key step for oncogenic development, which involves multiple pathways; however, the mechanism remains unclear. To test our hypothesis whether cell oncogenic transformation shares some mechanisms with the process of reprogramming non-stem cells to induced pluripotent stem cells (iPSC), we studied the relationship among the key factors for promoting or inhibiting iPSC in radiation-transformed human epithelial cell lines derived from different tissues (lung, breast and colon). We unexpectedly found that p63 and OCT4 were highly expressed (accompanied by low expressed p53 and miR-34a) in all transformed cell lines examined when compared with their non-transformed counterparts. We further elucidated the relationship of these factors: the 3p strand of miR-34a directly targeted OCT4 by binding to the 3′ untranslated region (3′-UTR) of OCT4 and, OCT4, in turn, stimulated p63 but inhibited p53 expression by binding to a specific region of the p63 or p53 promoter. Moreover, we revealed that the effects of OCT4 on promoting cell oncogenic transformation were by affecting p63 and p53. These results support that a positive loop exists in human cells: OCT4 upregulation as a consequence of inhibition of miR-34a, promotes p63 but suppresses p53 expression, which further stimulates OCT4 upregulation by downregulating miR-34a. This functional loop contributes significantly to cell transformation and, most likely, also to the iPSC process.
更多信息
| 物种 | 人类, 其它物种, 大鼠, 小鼠, 非人灵长类 |
|---|---|
| 克隆 | MC-813-70 |
| Gene Id | 330401 |
| Alternative Names | Stage-specific embryonic antigen-4 |
| 同种型/isotype | IgG3, kappa |
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