Anti-Human CD90 Antibody, Clone 5E10

总览

5E10抗体与CD90 （Thy-1）反应，CD90 是一种 GPI 锚定的膜糖蛋白，在两个位点进行 N-糖基化，生成分子量在 25–37 kDa 范围内的蛋白。CD90在信号转导、细胞粘附和迁移、神经突生长、T细胞活化、肿瘤抑制、造血干细胞增殖和分化抑制等方面发挥作用。它是已知的β2和β3整合素的配体，并上调纤维连接蛋白、骨连接蛋白和血小坂反应蛋白的合成。CD90广泛表达，存在于人胸腺细胞、神经元、部分神经胶质细胞、成纤维细胞、活化内皮细胞、某些白血病细胞系以及人外周血中一小部分（<1%）CD3+CD4+ T 细胞中。CD90还在胎肝、脐带血、骨髓和动员的外周血细胞中小部分 CD34+ 细胞中表达。CD90被认为是造血干细胞和祖细胞的重要标志物，与CD34等其他标志物结合，可用于流式细胞术（FACS）鉴定和分选这些细胞。

该抗体Clone已被验证用于标记使用MesenCult™增殖试剂盒(产品号：#05411)和MesenCult™-XF培养基（产品号：#05420）培养的人间充质细胞。

分类
一抗

靶抗原
CD90 (Thy-1)

别名
CDw90，Thy-1，Thy1

反应种属
狒狒、食蟹猴、犬、人、猕猴、猪、恒河猴

偶联
APC，生物素，FITC，PE，PerCP-Cyanine5.5，未偶联

宿主物种
小鼠

细胞类型
造血干/祖细胞，乳腺细胞，间充质干/祖细胞

种属
人，非人灵长类，其他物种

应用
CyTOF，ELISAs，流式细胞术，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀，Western印迹

研究领域
上皮细胞研究，干细胞生物学

Clone
5E10

Gene ID
7070

Isotype
IgG1, κ轻链

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实验数据

Figure 1. Data for Unconjugated

Flow cytometry analysis of human erythroleukemia (HEL) cells labeled with Anti-Human CD90 Antibody, Clone 5E10, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or a mouse IgG1, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).

Figure 2. Data for FITC-Conjugated

Flow cytometry analysis of human erythroleukemia (HEL) cells labeled with Anti-Human CD90 Antibody, Clone 5E10, FITC (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, FITC (Catalog #60070FI) (solid line histogram).

Figure 3. Data for PE-Conjugated

Flow cytometry analysis of human HEL cells labeled with Anti-Human CD90 Antibody, Clone 5E10, PE (filled histogram) or a mouse IgG1, kappa PE isotype control antibody (solid line histogram).

Figure 4. Data for APC-Conjugated

Flow cytometry analysis of human HEL cells labeled with Anti-Human CD90 Antibody, Clone 5E10, APC (filled histogram) or a mouse IgG1, kappa isotype control antibody, APC (solid line histogram).

Figure 5. Data for Biotin-Conjugated

Flow cytometry analysis of human HEL cells labeled with Anti-Human CD90 Antibody, Clone 5E10, Biotin followed by streptavidin (SAV) APC (filled histogram), or a biotinylated mouse IgG1, kappa isotype control antibody followed by SAV APC (solid line histogram).

Figure 6. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of human erythroleukemina (HEL) cells labeled with Anti-Human CD90 Antibody, Clone 5E10, PerCP-Cy5.5 (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PerCP-Cy5.5 (Catalog # 60070PS) (solid line histogram).