技术资料

产品手册
EasySep™ Direct 仅需20分钟，从全血中分选细胞

品牌：

EasySep
产品手册
非人灵长类细胞分选

品牌：

EasySep
实验方案
How to Thaw Frozen Primary Cells

研究方向：

免疫学，干细胞生物学，疾病建模，药物发现和毒性检测，传染病，癌症研究
科学海报
A Specialized Tube to Make Enrichment of Specific Cell Subsets Faster and Easier

Conference：

BSHI 2012,EFI 2012,IHIW 2012,AAI 2012
科学海报
Immunomagnetic Isolation Method for Mouse CD4+CD25+ Regulatory T Cells

Conference：

AAI 2008,CSI 2008
实验方案
How to Manually Dissociate Mouse Liver Tissue into a Single-Cell Suspension

研究方向：

免疫学
产品手册
GMP-Compliant Human Platelet Lysate Flyer

品牌：

MesenCult
科学海报
One-Step Enrichment of Leukocyte Subsets Directly in the Blood Collection Tube

Conference：

AAI 2003
产品手册
Isolate Human Immune Cells

品牌：

EasySep
技术窍门
Frequently Asked Questions on Primary Cells
实验方案
How to Differentiate hPSCs to Lymphoid-Competent CD34+ Hematopoietic Stem and/or Progenitor Cells Using a Scalable Suspension Protocol

研究方向：

免疫学，细胞治疗开发
Zhang L et al. (FEB 2016) Cell Reports 14 5 1206--17

Mammalian Target of Rapamycin Complex 2 Controls CD8 T Cell Memory Differentiation in a Foxo1-Dependent Manner.

Upon infection,antigen-specific naive CD8 T cells are activated and differentiate into short-lived effector cells (SLECs) and memory precursor cells (MPECs). The underlying signaling pathways remain largely unresolved. We show that Rictor,the core component of mammalian target of rapamycin complex 2 (mTORC2),regulates SLEC and MPEC commitment. Rictor deficiency favors memory formation and increases IL-2 secretion capacity without dampening effector functions. Moreover,mTORC2-deficient memory T cells mount more potent recall responses. Enhanced memory formation in the absence of mTORC2 was associated with Eomes and Tcf-1 upregulation,repression of T-bet,enhanced mitochondrial spare respiratory capacity,and fatty acid oxidation. This transcriptional and metabolic reprogramming is mainly driven by nuclear stabilization of Foxo1. Silencing of Foxo1 reversed the increased MPEC differentiation and IL-2 production and led to an impaired recall response of Rictor KO memory T cells. Therefore,mTORC2 is a critical regulator of CD8 T cell differentiation and may be an important target for immunotherapy interventions. View Publication

产品号#：

19858

19858RF

产品名：

EasySep™小鼠Naïve CD8+ T细胞分选试剂盒

RoboSep™ 小鼠Naïve CD8+ T细胞分选试剂盒