Belle K et al. (JAN 2017)
Neuroscience letters 637 201--206
Generation of disease-specific autopsy-confirmed iPSCs lines from postmortem isolated Peripheral Blood Mononuclear Cells
Understanding the molecular mechanisms that underlie neurodegenerative disorders has been hampered by a lack of readily available model systems that replicate the complexity of the human disease. Recent advances in stem cell technology have facilitated the derivation of patient-specific stem cells from a variety of differentiated cell types. These induced pluripotent stem cells (iPSCs) are attractive disease models since they can be grown and differentiated to produce large numbers of disease-relevant cell types. However,most iPSC lines are derived in advance of,and without the benefit of,neuropathological confirmation of the donor - the gold standard for many disease classifications and measurement of disease severity. While others have reported the generation of autopsy-confirmed iPSC lines from patient explants,these methods require outgrowth of cadaver tissue,which require additional time and is often only successul 50% of the time. Here we report the rapid generation of autopsy-confirmed iPSC lines from peripheral blood mononuclear cells (PBMCs) drawn postmortem. Since this approach doesn't require the propagation of previously frozen cadaver tissue,iPSC can be rapidly and efficiently produced from patients with autopsy-confirmed pathology. These matched iPSC-derived patient-specific neurons and postmortem brain tissue will support studies of specific mechanisms that drive the pathogenesis of neurodegenerative diseases.
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产品号#：
05833
05835
05839
07811
07861
18060
18061
85450
85460
85850
85857
86450
86460
产品名：
STEMdiff™神经前体细胞培养基
STEMdiff™ 神经诱导培养基
STEMdiff™ 神经诱导培养基
Lymphoprep™
Lymphoprep™
Lymphoprep™
Lymphoprep™
SepMate™-50 (IVD)
SepMate™-50 (IVD)
mTeSR™1
mTeSR™1
SepMate™-50 (RUO)
SepMate™-50 (RUO)
Robinson M et al. (AUG 2016)
Stem Cell Reviews and Reports 12 4 476--483
Functionalizing Ascl1 with Novel Intracellular Protein Delivery Technology for Promoting Neuronal Differentiation of Human Induced Pluripotent Stem Cells
Pluripotent stem cells can become any cell type found in the body. Accordingly,one of the major challenges when working with pluripotent stem cells is producing a highly homogenous population of differentiated cells,which can then be used for downstream applications such as cell therapies or drug screening. The transcription factor Ascl1 plays a key role in neural development and previous work has shown that Ascl1 overexpression using viral vectors can reprogram fibroblasts directly into neurons. Here we report on how a recombinant version of the Ascl1 protein functionalized with intracellular protein delivery technology (Ascl1-IPTD) can be used to rapidly differentiate human induced pluripotent stem cells (hiPSCs) into neurons. We first evaluated a range of Ascl1-IPTD concentrations to determine the most effective amount for generating neurons from hiPSCs cultured in serum free media. Next,we looked at the frequency of Ascl1-IPTD supplementation in the media on differentiation and found that one time supplementation is sufficient enough to trigger the neural differentiation process. Ascl1-IPTD was efficiently taken up by the hiPSCs and enabled rapid differentiation into TUJ1-positive and NeuN-positive populations with neuronal morphology after 8 days. After 12 days of culture,hiPSC-derived neurons produced by Ascl1-IPTD treatment exhibited greater neurite length and higher numbers of branch points compared to neurons derived using a standard neural progenitor differentiation protocol. This work validates Ascl1-IPTD as a powerful tool for engineering neural tissue from pluripotent stem cells.
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产品号#：
05832
05833
05835
05838
05839
07180
07183
07930
07931
07940
07952
07955
07959
08581
08582
100-0483
100-0484
100-0485
100-0763
100-1061
100-1077
36254
产品名：
STEMdiff™ 神经花环选择试剂
STEMdiff™神经前体细胞培养基
STEMdiff™ 神经诱导培养基
STEMdiff™神经祖细胞冻存液
STEMdiff™ 神经诱导培养基
Vitronectin XF™
CellAdhere™ 稀释缓冲液
CryoStor® CS10
CryoStor® CS10
CryoStor® CS10
CryoStor® CS10
CryoStor® CS10
CryoStor® CS10
STEMdiff™SMADi神经诱导试剂盒
STEMdiff™SMADi神经诱导试剂盒，2套
Hausser Scientificᵀᴹ 明线血球计数板
ReLeSR™
温和细胞解离试剂
Vitronectin XF™
CryoStor® CS10
ReLeSR™
DMEM/F-12 with 15 mM HEPES
Er JC et al. (FEB 2015)
Angewandte Chemie - International Edition 54 8 2442--2446
Neuo: A fluorescent chemical probe for live neuron labeling
To address existing limitations in live neuron imaging,we have developed NeuO,a novel cell-permeable fluorescent probe with an unprecedented ability to label and image live neurons selectively over other cells in the brain. NeuO enables robust live neuron imaging and isolation in vivo and in vitro across species; its versatility and ease of use sets the basis for its development in a myriad of neuronal targeting applications.
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EasySep™小鼠TIL（CD45）正选试剂盒
47:18
线上讲座Madeline Lancaster on Brain Organoids: Modeling Human Brain Development in a Dish发布日期: 09/20/2017
25:30
线上讲座Brains in a Dish: Using Cerebral Organoids to Study Human Brain Development and Disease发布日期: 07/03/2017
科学海报STEMdiff™ Cerebral Organoid Kit: A New Tool for the Culture of 3D Brain Organoids Derived from hPSCs
29:27
线上讲座Tired of Manual Neural Rosette Isolation? How to Form and Isolate More Efficiently发布日期: 04/24/2012
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