技术资料

A. Huber et al. (Jan 2023) STAR protocols 4 102076

Generation of gene-of-interest knockouts in murine organoids using CRISPR-Cas9.

Gene-of-interest knockout organoids present a powerful and versatile research tool to study a gene's effects on many biological and pathological processes. Here,we present a straightforward and broadly applicable protocol to generate gene knockouts in mouse organoids using CRISPR-Cas9 technology. We describe the processes of transient transfecting organoids with pre-assembled CRISPR-Cas9 ribonucleoprotein complexes,organoid cell sorting,and establishing clonal organoid culture pairs. We then detail how to confirm the knockout via Western blot analysis. View Publication

产品号#：

06005

产品名：

IntestiCult™ 类器官生长培养基 (小鼠)
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挂图
SnapShot: Growing Organoids from Stem Cells Key culture conditions and organoid-forming cells from a variety of epithelial tissues
技术公告
Forskolin诱导培养于IntestiCult™中人肠类器官膨胀的操作流程

细胞类型：

上皮细胞，肠道细胞
实验方案
如何使用 IntestiCult™ 培养小鼠肠类器官衍生的单层肠上皮

研究方向：

上皮细胞生物学，类器官
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技术资料

Generation of gene-of-interest knockouts in murine organoids using CRISPR-Cas9.

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