技术资料

科学海报
Mechanoporation-Based Intracellular Delivery of Functional Molecules for Primary Immune Cell Engineering

Conference：

AAI 2026
实验方案
如何通过密度梯度离心从全血中分离单个核细胞？

研究方向：

免疫学，嵌合体，传染病，癌症研究
Jin Q et al. (SEP 2011) Virology 417 2 449--56

Role for the conserved N-terminal cysteines in the anti-chemokine activities by the chemokine-like protein MC148R1 encoded by Molluscum contagiosum virus.

Molluscum contagiosum poxvirus (MCV) type 1 and type 2 encode two chemokine-like proteins MC148R1 and MC148R2. It is believed that MC148R proteins function by blocking the inflammatory response. However,the mechanism of the proposed biological activities of MC148R proteins and the role of the additional C-terminal cysteines that do not exist in other chemokines are not understood. Here,we demonstrated in two different assay systems that His-tagged MC148R1 displaces the interaction between CXCL12α and CXCR4. The N-terminal cysteines but not the additional C-terminal cysteines modulate this displacement. His-tagged MC148R1 blocked both CXCL12α-mediated and MIP-1α-mediated chemotaxis. In contrast,MC148R2 blocked MIP-1α-mediated but not CXCL12α-mediated chemotaxis. Immunoprecipitation by antibodies to MC148R1 or CXCL12α followed by immunoblotting and detection by antibodies to the other protein demonstrated physical interaction of His-tagged CXCL12α and His-tagged MC148R1. Interaction with chemokines might mask the receptor interaction site resulting in decreased binding and impairment of the biological activities. View Publication

产品号#：

70025

70025.1

70025.2

70025.3

70047

70047.1

70047.2

70048

70048.1

70048.2

产品名：

冻存的人外周血单个核细胞

冻存的人外周血单个核细胞

冻存的人外周血单个核细胞

冻存的人外周血单个核细胞
实验方案
How to Thaw Frozen Primary Cells

研究方向：

免疫学，干细胞生物学，疾病建模，药物发现和毒性检测，传染病，癌症研究
Lagadinou ED et al. (MAR 2013) Cell stem cell 12 3 329--41

BCL-2 inhibition targets oxidative phosphorylation and selectively eradicates quiescent human leukemia stem cells.

Most forms of chemotherapy employ mechanisms involving induction of oxidative stress,a strategy that can be effective due to the elevated oxidative state commonly observed in cancer cells. However,recent studies have shown that relative redox levels in primary tumors can be heterogeneous,suggesting that regimens dependent on differential oxidative state may not be uniformly effective. To investigate this issue in hematological malignancies,we evaluated mechanisms controlling oxidative state in primary specimens derived from acute myelogenous leukemia (AML) patients. Our studies demonstrate three striking findings. First,the majority of functionally defined leukemia stem cells (LSCs) are characterized by relatively low levels of reactive oxygen species (termed ROS-low"). Second� View Publication

产品号#：

07930

07931

07940

07955

07956

07959

07954

100-1061

07952

产品名：

CryoStor® CS10

CryoStor® CS10

CryoStor® CS10

CryoStor® CS10

CryoStor® CS10

CryoStor® CS10

CryoStor® CS10
实验方案
How to Collect Plasma from Whole Blood Before Cell Isolation

研究方向：

免疫学，疾病建模，细胞外囊泡研究，细胞治疗开发，癌症研究
实验方案
如何使用DNase I减少单细胞悬液中的细胞团块

研究方向：

免疫学，干细胞生物学
实验方案
Optimizing Delivery Efficiency with Fluorescent Dextran Using the CellPore™ Transfection System

研究方向：

免疫学，疾病建模，药物发现和毒性检测
产品手册
使用Easy 250 EasySep™ 磁极从大体积样本中分离目的细胞

品牌：

EasySep
产品手册
CellPore™ Mechanoporation Guide

品牌：

CellPore
科学海报
Optimized CRISPR-Cas9 Editing of Primary Human Immune and CD34+ Hematopoietic Stem and Progenitor Cells using a Mechanoporation Platform

Conference：

ASCGT 2025
产品手册
小鼠细胞分选

品牌：

EasySep