技术资料

点播课程
Expansion of hPSCs in 3D Suspension Culture Course
实验方案
Maintenance of Porcine Pluripotent Stem Cells (pPSCs) Using eTeSR™

研究方向：

干细胞生物学
M. R. Hildebrandt et al. (dec 2019) Stem cell reports 13 6 1126--1141

Precision Health Resource of Control iPSC Lines for Versatile Multilineage Differentiation.

Induced pluripotent stem cells (iPSC) derived from healthy individuals are important controls for disease-modeling studies. Here we apply precision health to create a high-quality resource of control iPSCs. Footprint-free lines were reprogrammed from four volunteers of the Personal Genome Project Canada (PGPC). Multilineage-directed differentiation efficiently produced functional cortical neurons,cardiomyocytes and hepatocytes. Pilot users demonstrated versatility by generating kidney organoids,T lymphocytes,and sensory neurons. A frameshift knockout was introduced into MYBPC3 and these cardiomyocytes exhibited the expected hypertrophic phenotype. Whole-genome sequencing-based annotation of PGPC lines revealed on average 20 coding variants. Importantly,nearly all annotated PGPC and HipSci lines harbored at least one pre-existing or acquired variant with cardiac,neurological,or other disease associations. Overall,PGPC lines were efficiently differentiated by multiple users into cells from six tissues for disease modeling,and variant-preferred healthy control lines were identified for specific disease settings. View Publication

产品号#：

05835

05839

05010

85850

85857

产品名：

STEMdiff™ 神经诱导培养基

STEMdiff™ 神经诱导培养基

STEMdiff™ 心室肌细胞分化试剂盒

mTeSR™1

mTeSR™1
实验方案
如何从小鼠脾脏制备单细胞悬液

研究方向：

免疫学，干细胞生物学
实验方案
How to Count Cell Spheres in Suspension Culture

研究方向：

干细胞生物学，药物发现和毒性检测，癌症研究
实验方案
How to Prepare and Plate Semi-Solid Methylcellulose Medium for Cell Culture

研究方向：

干细胞生物学，细胞系制备，药物发现和毒性检测
技术公告
Extracellular Vesicle Generation from Mesenchymal Stromal Cells Using MesenCult™-ACF Plus

细胞类型：

内皮细胞，间充质基质细胞，间充质干祖细胞
技术手册
Maintenance of Human Pluripotent Stem Cells in mTeSR™ Plus
实验方案
如何分选小鼠骨髓细胞并对其染色以进行流式检测？

研究方向：

干细胞生物学
实验方案
如何从多能干细胞生成T细胞

研究方向：

免疫学，干细胞生物学，细胞治疗开发