EasySep™ 总核酸提取试剂盒

磁珠法总核酸（DNA和RNA）提取试剂盒

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产品号 #（选择产品）

产品号 #100-1079_C

磁珠法总核酸（DNA和RNA）提取试剂盒

产品优势

大规模核酸提取，最多可同时提取16或96个样本
无需使用离心柱，避免离心步骤，节省样品处理时间
通过易于操作的体系简化工作流程，免去使用有毒试剂
实现稳定地从各种类型样本（如全血和细胞悬液）中提取核酸

产品组分包括

EasySep™总核酸浓缩RapidSpheres™，3mL（产品号#100-1091）
EasySep™总核酸裂解缓冲液，20mL（产品号#100-1090）
EasySep™总核酸蛋白酶K， 2mL（产品号#100-1092）
EasySep™总核酸RapidSpheres™稀释用瓶，1（产品号#100-1093）

立即询价

要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。

ErythroClear™磁极
96孔PCR磁力板

总览

实验数据

产品说明书及文档

应用领域

总览

高效地从全血、细胞悬液（如 PBMCs、hPSCs、培养细胞）及使用EasySep™ 分选的细胞中高效地提取总核酸（DNA 和 RNA）或仅提取 RNA。EasySep™ 总核酸提取试剂盒采用磁珠技术，配合简便且可扩展的操作流程，无需使用离心柱或有毒试剂。使用该试剂盒提取的核酸纯度高，可直接用于如qPCR等下游应用。

使用本试剂盒，可通过 EasySep™ 总核酸 RapidSpheres™ 磁珠对样本中的总核酸或 RNA 进行磁珠标记。随后无需分离柱，只需磁极（需单独购买）处理样本。去除未标记的杂质成分后，从磁极上取下样本并回收被磁珠标记的核酸。

该试剂盒提供了两种方案：使用 1.7 mL 微量离心管配合 ErythroClear™ 磁极（产品号 #01737）进行总核酸或 RNA 提取；或在 96 孔 PCR 板中配合96孔PCR磁力板（产品号 #100-1304）实现高通量提取。

磁极兼容性
ErythroClear™磁极（产品号 #01737）

96孔PCR微孔板磁极（产品号 #100-1304）

细胞类型
淋巴细胞，单个核细胞

应用
基因组编辑，核酸纯化

研究领域
癌症，免疫

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实验数据

Diagram of the EasySep™ Total Nucleic Acid Extraction Kit workflow.

Figure 1. EasySep™ Total Nucleic Acid Extraction Kit Workflow

Diagram of the standard extraction workflow. Time points at which samples must be placed in the magnet are indicated with gray boxes. EasySep™ Lysis Buffer and Proteinase K are added to the sample and incubated at 56°C for 10 minutes. Diluted EasySep™ Nucleic Acid RapidSpheres™ are added to the sample and incubated at room temperature (RT) for 5 minutes, then placed in the magnet for 2 minutes. While in the magnet, the sample is washed three times with a 70% ethanol wash solution, and the supernatant is removed. The pellet is resuspended with the elution buffer while removed from the magnet and incubated at room temperature for 5 minutes. The sample is then placed in the magnet for 2 minutes before the supernatant is aspirated to a new tube to obtain the extracted nucleic acids.

Figure 2. EasySep™ Total Nucleic Acid Extraction Kit Shows Improved DNA and RNA Recovery Relative to Other Commonly Used Methods

Normalized recovery (μg per 1 x 10^6 cells) of (A) DNA and (B) RNA across EasySep™ Total Nucleic Acid Extraction Kit and other commonly used extraction methods. DNA and RNA concentrations were measured separately using the Qubit™ Fluorometer. Sample source: Leukopak, n = 3. Error bars represent ± 1 standard deviation.

EasySep™ Total Nucleic Acid Extraction Kit recovers nucleic acids of optimal purity when benchmarked against other commonly used extraction methods.

Figure 3. EasySep™ Total Nucleic Acid Extraction Kit Recovers Nucleic Acid of Optimal Purity

Purity ratios obtained using spectrophotometric absorbance measurements show that the nucleic acid recovered by the EasySep™ Total Nucleic Acid Extraction Kit is of optimal purity, benchmarked against other commonly used extraction methods. The 260/280 ratio, indicative of protein, phenol, and other contaminants within the extract that absorb at or near 280 nm, has an optimum purity ratio of ~ 1.8 for DNA and ~ 2.0 for RNA. The 260/230 ratio, a secondary measure of purity indicative of salt, phenol, and other organic compound contamination, has an optimal range of 1.8 - 2.2. Ratios that fall outside of these ranges are generally considered contaminated. Purity ratios were obtained using the Nanodrop™ Spectrophotometer. Sample source: Leukopak, n = 3. Error bars represent ± 1 standard deviation.

Results from a DNA-based qPCR assay with primers targeted to a non-genic region of chromosome 4 demonstrate that, DNA is detectable in extractions with a starting cell input of 10 cells and up to 1 x 10^6 cells, using the EasySep™ Total Nucleic Acid Extraction Kit.

Figure 4. EasySep™ Total Nucleic Acid Extraction Kit Is Capable of Extracting from As Few As 10 Cells

Results from a DNA-based qPCR assay using primers targeted to a non-genic region of chromosome 4 demonstrate that DNA is detectable in extractions with a starting cell input of 10 - 1 x 10^6 cells. (A) qPCR curves coloured by cell input. Dotted red line represents the cycle threshold (Ct; 0.139). Each individual curve represents 1 technical replicate, n = 3. (B) Inverse linear relationship between log(cell number input) and Ct value. Each data point represents 1 technical replicate, n = 3. Sample source: Leukopak.