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Sarugaser R et al. ( 2009) Methods in molecular biology (Clifton,N.J.) 482 269--79

Isolation, propagation, and characterization of human umbilical cord perivascular cells (HUCPVCs).

Current sources of mesenchymal cells,including bone marrow,fat and muscle,all require invasive procurement procedures,and provide relatively low frequencies of progenitors. Here,we describe the non-invasive isolation,and characterization,of a rich source of mesenchymal progenitor cells,which we call human umbilical cord perivascular cells (HUCPVCs). HUCPVCs show a similar immunological phenotype to bone marrow-derived mesenchymal stromal cells (BM-MSCs),since they are non-alloreactive,exhibit immunosuppression,and significantly reduce lymphocyte activation,in vitro. They present a non-hematopoietic myofibroblastic mesenchymal phenotype (CD45-,CD34-,CD105+,CD73+,CD90+,CD44+,CD106+,3G5+,CD146+); with a 1:300 frequency at harvest,a short-doubling time,and a clonogenic frequency of textgreater1:3 in culture. Furthermore,in addition to robust quinti-potential differentiation capacity in vitro,HUCPVCs have been shown to contribute to both musculo-skeletal and dermal wound healing in vivo. View Publication

产品类型：

产品号#：

07930

07931

07940

07955

07956

07959

07954

100-1061

07952

产品名：

CryoStor® CS10

CryoStor® CS10

CryoStor® CS10

CryoStor® CS10

CryoStor® CS10

CryoStor® CS10

CryoStor® CS10
54:41

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Panel Discussion: Balancing Speed, Safety, and Scale in hPSC-Derived Therapy Development

发布日期: 03/05/2026

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搜索结果: 'cryostor'

Isolation, propagation, and characterization of human umbilical cord perivascular cells (HUCPVCs).

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