搜索结果: 'gmp t cell activator'

Porayette P et al. (DEC 2007) Biochemical and biophysical research communications 364 3 522--527

Amyloid-?? precursor protein expression and modulation in human embryonic stem cells: A novel role for human chorionic gonadotropin

The amyloid-beta precursor protein (AbetaPP) is a ubiquitously expressed adhesion and neuritogenic protein whose processing has previously been shown to be regulated by reproductive hormones including the gonadotropin luteinizing hormone (LH) in human neuroblastoma cells. We report for the first time the expression of AbetaPP in human embryonic stem (hES) cells at the mRNA and protein levels. Using N- and C-terminal antibodies against AbetaPP,we detected both the mature and immature forms of AbetaPP as well as truncated variants ( approximately 53kDa,47kDa,and 29kDa) by immunoblot analyses. Expression of AbetaPP is regulated by both the stemness of the cells and pregnancy-associated hormones. Addition of human chorionic gonadotropin,the fetal equivalent of LH that is dramatically elevated during pregnancy,markedly increased the expression of all AbetaPP forms. These results indicate a critical molecular signaling link between the hormonal environment of pregnancy and the expression of AbetaPP in hES cells that is suggestive of an important function for this protein during early human embryogenesis prior to the formation of neural precursor cells. View Publication

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产品号#：

05850

05857

05870

05875

85850

85857

85870

85875

产品名：

mTeSR™1

Qiu C et al. (FEB 2008) Blood 111 4 2400--8

Globin switches in yolk sac-like primitive and fetal-like definitive red blood cells produced from human embryonic stem cells.

We have previously shown that coculture of human embryonic stem cells (hESCs) for 14 days with immortalized fetal hepatocytes yields CD34(+) cells that can be expanded in serum-free liquid culture into large numbers of megaloblastic nucleated erythroblasts resembling yolk sac-derived cells. We show here that these primitive erythroblasts undergo a switch in hemoglobin (Hb) composition during late terminal erythroid maturation with the basophilic erythroblasts expressing predominantly Hb Gower I (zeta(2)epsilon(2)) and the orthochromatic erythroblasts hemoglobin Gower II (alpha(2)epsilon(2)). This suggests that the switch from Hb Gower I to Hb Gower II,the first hemoglobin switch in humans is a maturation switch not a lineage switch. We also show that extending the coculture of the hESCs with immortalized fetal hepatocytes to 35 days yields CD34(+) cells that differentiate into more developmentally mature,fetal liver-like erythroblasts,that are smaller,express mostly fetal hemoglobin,and can enucleate. We conclude that hESC-derived erythropoiesis closely mimics early human development because the first 2 human hemoglobin switches are recapitulated,and because yolk sac-like and fetal liver-like cells are sequentially produced. Development of a method that yields erythroid cells with an adult phenotype remains necessary,because the most mature cells that can be produced with current systems express less than 2% adult beta-globin mRNA. View Publication

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09600

09650

18056

18056RF

产品名：

StemSpan™ SFEM

Zang Y et al. (MAR 2008) The Journal of biological chemistry 283 10 6201--8

AICAR induces astroglial differentiation of neural stem cells via activating the JAK/STAT3 pathway independently of AMP-activated protein kinase.

Neural stem cell differentiation and the determination of lineage decision between neuronal and glial fates have important implications in the study of developmental,pathological,and regenerative processes. Although small molecule chemicals with the ability to control neural stem cell fate are considered extremely useful tools in this field,few were reported. AICAR is an adenosine analog and extensively used to activate AMP-activated protein kinase (AMPK),a metabolic fuel gauge" of the biological system. In the present study� View Publication

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72704

产品名：

AICAR

Ghiaur G et al. (APR 2008) Blood 111 7 3313--21

Rac1 is essential for intraembryonic hematopoiesis and for the initial seeding of fetal liver with definitive hematopoietic progenitor cells.

Definitive hematopoietic stem and progenitor cells (HSCs/Ps) originating from the yolk sac and/or para-aorta-splanchno-pleura/aorta-gonad-mesonephros are hypothesized to colonize the fetal liver,but mechanisms involved are poorly defined. The Rac subfamily of Rho GTPases has been shown to play essential roles in HSC/P localization to the bone marrow following transplantation. Here,we study the role of Rac1 in HSC/P migration during ontogeny and seeding of fetal liver. Using a triple-transgenic approach,we have deleted Rac1 in HSCs/Ps during very early embryonic development. Without Rac1,there was a decrease in circulating HSCs/Ps in the blood of embryonic day (E) 10.5 embryos,while yolk sac definitive hematopoiesis was quantitatively normal. Intraembryonic hematopoiesis was significantly impaired in Rac1-deficient embryos,culminating with absence of intra-aortic clusters and fetal liver hematopoiesis. At E10.5,Rac1-deficient HSCs/Ps displayed decreased transwell migration and impaired inter-action with the microenvironment in migration-dependent assays. These data suggest that Rac1 plays an important role in HSC/P migration during embryonic development and is essential for the emergence of intraembryonic hematopoiesis. View Publication

产品类型：

产品号#：

03134

09600

09650

产品名：

MethoCult™ M3134

StemSpan™ SFEM

技术窍门

Vitronectin XF™ Defined Matrix for Human Pluripotent Stem Cells

发布日期: 12/20/2012

新闻

STEMCELL Technologies Signs Exclusive Agreement with the Institute of Molecular Biotechnology to Commercialize Cerebral Organoid Culture Systems

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发布日期: 04/13/2016

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RSeT™ For Naive-Like Human Pluripotent Stem Cells

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品牌：

RSeT

产品号#：

05970

00226

00227

产品名：

发布日期: 06/01/2016

新闻

NEW PneumaCult™-ALI Medium for Air-Liquid Interface Culture of Bronchial Epithelial Cells

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发布日期: 06/05/2012

新闻

STEMCELL Technologies Applauds Feeder-free Derivation of Human Induced Pluripotent Stem Cells

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发布日期: 09/09/2009

新闻

Now Available! Isolate Untouched Human Plasmacytoid Dendritic Cells (pDCs) with EasySep™ Human pDC Enrichment Kit (Catalog #19062)

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发布日期: 01/16/2009

Making Lung Cells from Pluripotent Stem Cells: Disease Modeling and Future Therapies

44:40

线上讲座

Making Lung Cells from Pluripotent Stem Cells: Disease Modeling and Future Therapies

发布日期: 08/12/2016

产品手册

MesenCult™-hPL Medium For the Derivation and Expansion of Human Mesenchymal Stem and Progenitor Cells

产品类型：

品牌：

MesenCult

产品号#：

05439

产品名：

MesenCult™-hPL 培养基试剂盒

发布日期: 07/01/2016

搜索结果: 'gmp t cell activator'

Amyloid-?? precursor protein expression and modulation in human embryonic stem cells: A novel role for human chorionic gonadotropin

Globin switches in yolk sac-like primitive and fetal-like definitive red blood cells produced from human embryonic stem cells.

AICAR induces astroglial differentiation of neural stem cells via activating the JAK/STAT3 pathway independently of AMP-activated protein kinase.

Rac1 is essential for intraembryonic hematopoiesis and for the initial seeding of fetal liver with definitive hematopoietic progenitor cells.

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