搜索结果: 'gmp t cell activator'

技术窍门

Acquiring DNA or RNA from Positively Selected Cells

发布日期: 02/23/2011

技术窍门

Isolating Cells from Atypical Samples

发布日期: 09/30/2013

新闻

STEMCELL Technologies Will Test BioLife Solutions' evo™ Smart Shipper and Cloud Based biologistex™ Cold Chain Management App

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发布日期: 01/06/2016

技术公告

Myogenic Progenitor and Satellite Cells

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细胞类型：

肌源干祖细胞

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发布日期: 06/01/2015

新闻

Dr. Allen Eaves of STEMCELL Technologies Inc. named EY Entrepreneur Of The Year™ Pacific 2016

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发布日期: 10/01/2016

Modeling Human Gastrointestinal Development and Disease Using Pluripotent Stem Cells

56:31

线上讲座

Modeling Human Gastrointestinal Development and Disease Using Pluripotent Stem Cells

发布日期: 03/06/2017

科学海报

Positive Selection of PE- or Biotin-Conjugated Antibody Labeled Cells with Releasable Rapidspheres™

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Conference：

AAI 2017

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17654

17656

17655

17653

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EasySep™ Release人PE正选试剂盒

EasySep™ Release小鼠PE正选试剂盒

EasySep™ Release小鼠Biotin正选试剂盒

EasySep™ Release人Biotin正选试剂盒

技术手册

Maintenance of Human Pluripotent Stem Cells in TeSR™-E8™

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产品号#：

05990

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用于hESC/hiPSC维持培养的TeSR™-E8™

技术手册

Reversion and Maintenance of Naïve-Like Human Pluripotent Stem Cells with NaïveCult™ Induction Kit and NaïveCult™ Expansion Medium

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产品号#：

05580

05590

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用于hPSCs重置为原始状态的NaïveCult™诱导试剂盒

NaïveCult™扩增培养基

Gentry T and Smith C (AUG 1999) Experimental hematology 27 8 1244--54

Retroviral vector-mediated gene transfer into umbilical cord blood CD34brCD38-CD33- cells.

In this report,we sought to optimize gene transfer into primitive human umbilical cord blood (UCB) cells. Initially,we found that fresh UCB isolated with the CD34brCD38 CD33 phenotype were highly enriched for hematopoietic progenitors detected in extended long-term cultures (8-week LTCs). In addition,following ex vivo gene transfer,this population possessed virtually all the 8-week LTC activity of the cultured cells. A multiparameter FACS assay was developed to efficiently screen the effects of alternative retroviral vector gene transfer procedures on the transduction efficiency and maintenance of CD34brCD38 CD33 cells. Proliferation of the CD34brCD38 CD33 cells was found to be a prerequisite for efficient transduction. However,in all conditions tested,proliferation of the CD34brCD38 CD33 cells was associated with a progressive loss of primitive cell properties including a reduction in CD34 expression,an increase in CD38/CD33 expression,and a decline in the ability to sustain 8-week LTCs. These observations indicate that it will be necessary to define conditions that more effectively support the self-renewal capacity of CD34brCD38 CD33 cells to optimize retroviral vector gene transfer in these cells. Evaluating these conditions and reagents will be facilitated by the multiparameter FACS assay described in this report. View Publication

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04431

产品名：

MethoCult™ H4431

Muraille E et al. (SEP 1999) The Biochemical journal 342 Pt 3 697--705

Distribution of the src-homology-2-domain-containing inositol 5-phosphatase SHIP-2 in both non-haemopoietic and haemopoietic cells and possible involvement of SHIP-2 in negative signalling of B-cells.

The termination of activation signals is a critical step in the control of the immune response; perturbation of inhibitory feedback pathways results in profound immune defects culminating in autoimmunity and overwhelming inflammation. FcgammaRIIB receptor is a well described inhibitory receptor. The ligation of B-cell receptor (BCR) and FcgammaRIIB leads to the inhibition of B-cell activation. Numerous studies have demonstrated that the SH2-domain-containing inositol 5-phosphatase SHIP (referred hereto as SHIP-1) is essential in this process. The cDNA encoding a second SH2-domain-containing inositol 5-phosphatase,SHIP-2,has been cloned [Pesesse,Deleu,De Smedt,Drayer and Erneux (1997) Biochem. Biophys. Res. Commun. 239,697-700]. Here we report the distribution of SHIP-2 in mouse tissues: a Western blot analysis of mouse tissues reveals that SHIP-2 is expressed in both haemopoietic and non-haemopoietic cells. In addition to T-cell and B-cell lines,spleen,thymus and lung are shown to coexpress SHIP-1 and SHIP-2. Moreover,SHIP-2 is detected in fibroblasts,heart and different brain areas. SHIP-2 shows a maximal tyrosine phosphorylation and association to Shc after ligation of BCR to FcgammaRIIB but not after stimulation of BCR alone. Our results therefore suggest a possible role for SHIP-2 in the negative regulation of immunocompetent cells. View Publication

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01508

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Galy A et al. (JAN 2000) Blood 95 1 128--37

Distinct signals control the hematopoiesis of lymphoid-related dendritic cells.

The molecular and cellular requirements for the development of different populations of human dendritic cells (DC) were studied. Conditions were defined that support DC production from lymphoid progenitors but that fail to induce DC formation from peripheral monocytes. The production of these lymphoid-related DC was severely blocked when hematopoietic progenitors overexpressed Ik7,a mutant dominant-negative Ikaros protein. In contrast,Ik7 did not block the formation of DC in conditions supporting the development of monocyte-derived DC. Furthermore,Ik7 did not block the formation of monocyte/macrophages and enhanced granulopoiesis. One of the molecular mechanisms mediated by Ik7 appears to be down-regulation of the flt3-receptor mRNA. Thus,distinct signals control the formation of DC demonstrating that some aspects of DC diversity are determined in part by distinct molecular cues at the hematopoietic level. (Blood. 2000;95:128-137) View Publication

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产品号#：

04431

产品名：

MethoCult™ H4431

搜索结果: 'gmp t cell activator'

Retroviral vector-mediated gene transfer into umbilical cord blood CD34brCD38-CD33- cells.

Distribution of the src-homology-2-domain-containing inositol 5-phosphatase SHIP-2 in both non-haemopoietic and haemopoietic cells and possible involvement of SHIP-2 in negative signalling of B-cells.

Distinct signals control the hematopoiesis of lymphoid-related dendritic cells.

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