搜索结果: 'methocult media formulations for human hematopoietic cells serum containing'

重组人TGF - β 2 转化生长因子β 2
重组人VEGF-165 血管内皮生长因子，不含动物成分
Kunova M et al. (NOV 2010) Reproductive biomedicine online 21 5 676--86

Development of humanized culture medium with plant-derived serum replacement for human pluripotent stem cells.

For human embryonic stem cells (ESC) to be used in cell replacement therapies,they must be grown under good manufacturing conditions in a chemically defined medium that lacks animal proteins. This study examined the ability of a newly designed medium containing the plant-derived serum replacement VegetaCell and other reagents of human origin to support undifferentiated growth and pluripotency of human ESC. This medium was tested in several culture systems,using human fibroblasts as a feeder layer or Matrigel in a feeder-free culture. Even under the most stringent feeder-free conditions without conditioned medium,human ESC exhibited an undifferentiated morphology,expressed markers of undifferentiated cells,demonstrated high alkaline phosphatase activity and multilineage differentiation and retained a normal karyotype. Compared with human ESC grown in standard culture conditions,human ESC maintained in humanized VegetaCell medium show longer cell cycles and decreased cell death. The availability of an animal protein-free medium supplemented with the low-cost VegetaCell reagent expands the repertoire of media for culturing human ESC as well as induced pluripotent stem cells for drug testing and cell replacement therapy. View Publication

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Niwa A et al. (JAN 2011) PLoS ONE 6 7 e22261

A novel Serum-Free monolayer culture for orderly hematopoietic differentiation of human pluripotent cells via mesodermal progenitors

Elucidating the in vitro differentiation of human embryonic stem (ES) and induced pluripotent stem (iPS) cells is important for understanding both normal and pathological hematopoietic development in vivo. For this purpose,a robust and simple hematopoietic differentiation system that can faithfully trace in vivo hematopoiesis is necessary. In this study,we established a novel serum-free monolayer culture that can trace the in vivo hematopoietic pathway from ES/iPS cells to functional definitive blood cells via mesodermal progenitors. Stepwise tuning of exogenous cytokine cocktails induced the hematopoietic mesodermal progenitors via primitive streak cells. These progenitors were then differentiated into various cell lineages depending on the hematopoietic cytokines present. Moreover,single cell deposition assay revealed that common bipotential hemoangiogenic progenitors were induced in our culture. Our system provides a new,robust,and simple method for investigating the mechanisms of mesodermal and hematopoietic differentiation. View Publication

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mTeSR™1

mTeSR™1
产品手册
StemSpan™: Defined Media and Supplements for Hematopoietic Cell Expansion

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StemSpan

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StemSpan™CC100

StemSpan™CD34+扩增补充（10X）

StemSpan™髓系扩增补品（100X）

StemSpan™巨核细胞扩增添加物 (100X)

StemSpan™CC110

StemSpan™红系扩增添加物 (100X)

StemSpan™ SFEM

StemSpan™ SFEM

StemSpan™ SFEM II

StemSpan™ SFEM II
技术公告
Genome Editing of Human CD34+ Hematopoietic Stem and Progenitor Cells Using the ArciTect™ CRISPR-Cas9 System and StemSpan™ Media

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200-0013

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ArciTect™ sgRNA
ArciTect™ 人HPRT 阳性对照试剂盒 CRISPR-Cas9 基因组编辑的阳性对照
EasySep™人CD15正选试剂盒
对来源于人全血的 CD15⁺ 细胞进行免疫磁珠正选分离
EasySep™人髓样DC富集试剂盒 免疫磁珠负选人髓样DCs
细胞外囊泡人CD9/CD63/CD81抗体组合 使用 CD9、CD63 和 CD81 标记物检测细胞外囊泡的抗体试剂盒
人淀粉样蛋白-β（1-42）肽（三氟乙酸盐） 神经毒性肽片段
人IgM抗体对ELISA试剂盒 用于人免疫球蛋白M的检测和测定