搜索结果: 'methocult media formulations for mouse hematopoietic cells serum containing'

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产品手册
MethoCult™ Media for Performing Hematopoietic Colony-Forming Unit (CFU) Assays

产品类型：

品牌：

MethoCult

产品号#：

00217

00217UK

00215

00215GER

00215UK

03534

03630

03134

03231

03234

03334

03434

03444

03436

04436

04064

04100

04230

04236

04431

04434

04444

04464

04531

04535

04545

04536

04564

07700

07800

07850

27100

27150

27140

27141

27500

38068

28110

28120

28230

28240

03

产品名：

MethoCult™ GF M3534

MethoCult™ M3630

MethoCult™ M3134

MethoCult™ M3231

MethoCult™ M3234

MethoCult™ M3334

MethoCult™ GF M3434

MethoCult™ GF M3434

MethoCult™ SF M3436

MethoCult™ SF H4436

MethoCult™ H4034 Optimum 入门试剂盒

MethoCult™ H4100

MethoCult™ H4230

MethoCult™ SF H4236

MethoCult™ H4431

MethoCult™ H4434 Classic

MethoCult™ H4434 Classic

MethoCult™ H4434 Classic 套装

MethoCult™ H4531

MethoCult™ H4535 Enriched，不含EPO

MethoCult™ H4535 Enriched，不含EPO

MethoCult™ SF H4536

MethoCult™ H4534 Classic 无 EPO 入门试剂盒

含 2% FBS的Iscove's MDM

氯化铵溶液

氯化铵溶液

35 mm培养皿

35 mm培养皿

Corning® 60 mm 带网格培养皿

16号钝针

16号钝针

3 mL注射器

3 mL注射器
技术公告
MethoCult™ Formulation Optimized for Mouse BFU-E Detection

产品类型：

细胞类型：

造血干祖细胞

产品号#：

00217

00217UK

03231

03234

03334

03434

03444

03436

03236

00217CA.3

00217UK.3

产品名：

MethoCult™ M3231

MethoCult™ M3234

MethoCult™ M3334

MethoCult™ GF M3434

MethoCult™ GF M3434

MethoCult™ SF M3436

MethoCult™ SF M3236

发布日期: 11/01/2009
Limó et al. (NOV 1997) Blood 90 9 3316--21

High-titer retroviral vectors containing the enhanced green fluorescent protein gene for efficient expression in hematopoietic cells.

Retroviral vectors constitute the most efficient system to deliver and integrate foreign genes into mammalian cells. We have developed a producer cell line that yields high titers of amphotropic retroviral vectors carrying the enhanced green fluorescent protein (EGFP) gene,a codon humanized,red-shifted variant of the green fluorescent protein (GFP) gene,which can be used as a selectable marker. We have used a hybrid vector that has been shown to efficiently drive gene expression in hematopoietic cells. Virtually all murine and human cell lines and primary human hematopoietic cells tested were transduced with varying efficiency after incubation with vector-containing supernatants. Human CD34(+) cells obtained from cord blood or aphereses products were transduced using a protocol that involves daily addition of vector-containing supernatants for 6 consecutive days. At day 6,up to 16% of the cells expressed EGFP,as assessed by flow cytometry. Sorted EGFP-expressing cells were able to produce fluorescent hematopoietic colonies. EGFP's main advantages are its fast flow cytometry determination and the possibility of cell sorting and simultaneous evaluation of the transduction efficiency along with other phenotypic markers. View Publication

产品类型：

产品号#：

04034

04044

产品名：

MethoCult™ H4034 Optimum

MethoCult™ H4034 Optimum
技术公告
Flow Cytometry Methods for Identifying Mouse Hematopoietic Stem and Progenitor Cells

产品类型：

细胞类型：

造血干祖细胞

产品号#：

产品名：

发布日期: 08/31/2018