搜索结果: 'methocult media formulations for human hematopoietic cells serum containing'

RosetteSep™人粒细胞去除抗体混合物 免疫密度负选试剂混合物
冻存的人外周血嗜酸性粒细胞 冻存的人原代细胞
冻存的人外周血白细胞单采术样本 冻存的人原代细胞
冻存的人外周血单核细胞 冻存的人原代细胞
冻存的人外周血中性粒细胞 冻存的人原代细胞
人重组加脂联素（gAcrp30） 脂联素球状结构域，30 kDa脂肪细胞成分相关蛋白，无动物源成分
新品
人重组IGF-I LR3，ACF 胰岛素样生长因子1长精氨酸3，无动物成分
重组人SCF（大肠杆菌表达） 干细胞因子
ImmunoCult™ 人Th1分化添加剂 人naïve CD4+ T细胞向Th1细胞分化的无血清培养添加剂
MesenCult™ MSC 刺激补充剂（人） CFU-F检测和人间充质干细胞扩增的补充剂
技术公告
MethoCult™ for Rat Hematopoietic Colony-Forming Unit (CFU) Assays

产品类型：

细胞类型：

造血干/祖细胞

产品号#：

03436

03774

产品名：

MethoCult™SF M3436

MethoCult™GF R3774
Limó et al. (NOV 1997) Blood 90 9 3316--21

High-titer retroviral vectors containing the enhanced green fluorescent protein gene for efficient expression in hematopoietic cells.

Retroviral vectors constitute the most efficient system to deliver and integrate foreign genes into mammalian cells. We have developed a producer cell line that yields high titers of amphotropic retroviral vectors carrying the enhanced green fluorescent protein (EGFP) gene,a codon humanized,red-shifted variant of the green fluorescent protein (GFP) gene,which can be used as a selectable marker. We have used a hybrid vector that has been shown to efficiently drive gene expression in hematopoietic cells. Virtually all murine and human cell lines and primary human hematopoietic cells tested were transduced with varying efficiency after incubation with vector-containing supernatants. Human CD34(+) cells obtained from cord blood or aphereses products were transduced using a protocol that involves daily addition of vector-containing supernatants for 6 consecutive days. At day 6,up to 16% of the cells expressed EGFP,as assessed by flow cytometry. Sorted EGFP-expressing cells were able to produce fluorescent hematopoietic colonies. EGFP's main advantages are its fast flow cytometry determination and the possibility of cell sorting and simultaneous evaluation of the transduction efficiency along with other phenotypic markers. View Publication

产品类型：

产品号#：

04034

04044

产品名：

MethoCult™H4034 Optimum

MethoCult™H4034 Optimum